Aluminum-Enhanced Proton Release Associated with Plasma Membrane H+-Adenosine Triphosphatase Activity and Excess Cation Uptake in Tea (Camellia sinensis) Plant Roots

Pedosphere ◽  
2018 ◽  
Vol 28 (5) ◽  
pp. 804-813 ◽  
Author(s):  
Qing WAN ◽  
Yuhe MI ◽  
Yiyang YANG ◽  
Xu Renkou ◽  
Xinghui LI
Keyword(s):  
Plasma Membrane ◽  
Camellia Sinensis ◽  
Adenosine Triphosphatase ◽  
Plant Roots ◽  
Proton Release ◽  
Adenosine Triphosphatase Activity ◽  
Cation Uptake

scholarly journals PROBLEMS ASSOCIATED WITH THE DEMONSTRATION BY LEAD METHODS OF ADENOSINE TRIPHOSPHATASE ACTIVITY IN RESIDENT PERITONEAL MACROPHAGES AND EXUDATE MONOCYTES OF THE GUINEA PIG

1973 ◽  
Vol 21 (5) ◽  
pp. 488-498 ◽  
Author(s):  
R. E. POELMANN ◽  
W. T. DAEMS ◽  
E. J. VAN LOHUIZEN
Keyword(s):  
Plasma Membrane ◽  
Guinea Pig ◽  
Microscopic Study ◽  
Heterogeneous Nucleation ◽  
Adenosine Triphosphatase ◽  
Peritoneal Macrophages ◽  
Electron Microscopic ◽  
Adenosine Triphosphatase Activity ◽  
Eosinophilic Granulocytes ◽  
Membrane Bound

This cytochemical and electron microscopic study on peritoneal macrophages of the guinea pig has raised doubts concerning the validity of lead methods for the demonstration of plasma membrane-bound adenosine triphosphatase activity. The problems encountered are inherent in the use of lead ions as a capture reagent. The nonenzymatically formed precipitates reflect sites of heterogeneous nucleation specific for certain kinds of cells, e.g., resident peritoneal macrophages, eosinophilic granulocytes and, to a lesser degree, exudate monocytes. This type of precipitation is also catalyzed on the surface of nonbiologic matrices such as latex particles. Enzymatic processes may well occur, but they cannot be distinguished from nonenzymatic processes.

scholarly journals ULTRASTRUCTURAL LOCALIZATION OF Mg++-DEPENDENT DINITROPHENOL-STIMULATED ADENOSINE TRIPHOSPHATASE IN HUMAN BLOOD PLATELETS

1967 ◽  
Vol 15 (5) ◽  
pp. 267-272 ◽  
Author(s):  
VICTOR G. VETHAMANY ◽  
SYDNEY S. LAZARUS
Keyword(s):  
Enzyme Activity ◽  
Plasma Membrane ◽  
Human Blood ◽  
Adenosine Triphosphatase ◽  
Blood Platelets ◽  
Ultrastructural Localization ◽  
Human Platelets ◽  
Structural Localization ◽  
Adenosine Triphosphatase Activity ◽  
Human Blood Platelets

Fine structural localization of adenosine triphosphatase activity was studied in human platelets briefly fixed in cold formol calcium and then incubated in lead medium with added dinitrophenol. Under these conditions, the Mg++-dependent dinitrophenol-stimulated adenosine triphosphatase of platelet mitochondria was demonstrated, but neither granules nor plasma membrane showed enzyme activity.

Proton release from tea plant (Camellia sinensis L.) roots induced by Al(III) under hydroponic conditions

Soil Research ◽  
2012 ◽  
Vol 50 (6) ◽  
pp. 482 ◽  
Author(s):  
Qing Wan ◽  
Ren-kou Xu ◽  
Xing-hui Li
Keyword(s):  
Camellia Sinensis ◽  
Soil Acidification ◽  
Solution Culture ◽  
Tea Plant ◽  
Plant Roots ◽  
Proton Release ◽  
Constant Ph ◽  
Initial Ph ◽  
Tea Plants ◽  
Hydroponic Conditions

The mechanisms for soil acidification induced by tea plant growth are not well understood. Proton release from tea plant (Camellia sinensis L.) roots induced by aluminium (Al(III)) in solution-culture experiments was examined with an automatic titration system, to determine the effect of Al(III) uptake by the plants on soil acidification. Results indicated that the uptake of Al(III) by tea plants led to proton release from their roots and thus an increase in soil acidification. The uptake of Al(III) by tea plants and the amount of protons released from the roots were greater at pH 4.5 than at pH 5.0 and 4.0 and increased with increasing initial Al(III) concentration in the culture solutions. With the same initial pH, the amount of protons released from tea plant roots at a constant pH was much higher than that at non-constant pH. The presence of ammonium increased the amount of protons released from tea plant roots. Therefore, the uptake of Al by tea plants and subsequent release of protons from their roots may be an important mechanism by which they acidify soils in tea gardens.

Sign in / Sign up

Export Citation Format

Share Document