Blue Native electrophoresis to study mitochondrial and other protein complexes

Methods ◽  
2002 ◽  
Vol 26 (4) ◽  
pp. 327-334 ◽  
Author(s):  
L Nijtmans
Keyword(s):  
Protein Complexes ◽  
Native Electrophoresis ◽  
Blue Native Electrophoresis ◽  
Blue Native

Blue native electrophoresis as a tool for detection of PIP-containing protein complexes in the plasma membranes

2013 ◽  
Vol 60 (4) ◽  
pp. 563-570
Author(s):  
T. A. Shevyreva ◽  
M. S. Piotrovskii ◽  
B. V. Belugin ◽  
I. M. Zhestkova ◽  
M. S. Trofimova
Keyword(s):  
Plasma Membranes ◽  
Protein Complexes ◽  
Native Electrophoresis ◽  
Blue Native Electrophoresis ◽  
Blue Native

Quantification of Protein Complexes by Blue Native Electrophoresis

2013 ◽  
pp. 363-379 ◽  
Author(s):  
Juliana Heidler ◽  
Valentina Strecker ◽  
Florian Csintalan ◽  
Lea Bleier ◽  
Ilka Wittig
Keyword(s):  
Protein Complexes ◽  
Native Electrophoresis ◽  
Blue Native Electrophoresis ◽  
Blue Native

LC-nanospray-MS/MS analysis of hydrophobic proteins from membrane protein complexes isolated by blue-native electrophoresis

2005 ◽  
Vol 40 (9) ◽  
pp. 1223-1231 ◽  
Author(s):  
Anabel S. Fandiño ◽  
Isam Rais ◽  
Martin Vollmer ◽  
Helmuth Elgass ◽  
Hermann Schägger ◽  
...  
Keyword(s):  
Membrane Protein ◽  
Protein Complexes ◽  
Ms Analysis ◽  
Native Electrophoresis ◽  
Membrane Protein Complexes ◽  
Blue Native Electrophoresis ◽  
Blue Native

scholarly journals Tim18p Is a New Component of the Tim54p-Tim22p Translocon in the Mitochondrial Inner Membrane

2000 ◽  
Vol 11 (1) ◽  
pp. 103-116 ◽  
Author(s):  
Oliver Kerscher ◽  
Naresh B. Sepuri ◽  
Robert E. Jensen
Keyword(s):  
Growth Defect ◽  
Inner Membrane ◽  
New Members ◽  
Mitochondrial Inner Membrane ◽  
Native Electrophoresis ◽  
Multiple Copies ◽  
New Gene ◽  
Synthetically Lethal ◽  
Blue Native Electrophoresis ◽  
Blue Native

The mitochondrial inner membrane contains two separate translocons: one required for the translocation of matrix-targeted proteins (the Tim23p-Tim17p complex) and one for the insertion of polytopic proteins into the mitochondrial inner membrane (the Tim54p-Tim22p complex). To identify new members of the Tim54p-Tim22p complex, we screened for high-copy suppressors of the temperature-sensitivetim54-1 mutant. We identified a new gene,TIM18, that encodes an integral protein of the inner membrane. The following genetic and biochemical observations suggest that the Tim18 protein is part of the Tim54p-Tim22p complex in the inner membrane: multiple copies of TIM18 suppress thetim54-1 growth defect; thetim18::HIS3 disruption is synthetically lethal with tim54-1; Tim54p and Tim22p can be coimmune precipitated with the Tim18 protein; and Tim18p, along with Tim54p and Tim22p, is detected in an ∼300-kDa complex after blue native electrophoresis. We propose that Tim18p is a new component of the Tim54p-Tim22p machinery that facilitates insertion of polytopic proteins into the mitochondrial inner membrane.

Sign in / Sign up

Export Citation Format

Share Document