Four Novel Mycoviruses from the Hypovirulent Botrytis cinerea SZ-2-3y Isolate from Paris polyphylla: Molecular Characterisation and Mitoviral Sequence Transboundary Entry into Plants

A hypovirulent SZ-2-3y strain isolated from diseased Paris polyphylla was identified as Botrytis cinerea. Interestingly, SZ-2-3y was coinfected with a mitovirus, two botouliviruses, and a 3074 nt fusarivirus, designated Botrytis cinerea fusarivirus 8 (BcFV8); it shares an 87.2% sequence identity with the previously identified Botrytis cinerea fusarivirus 6 (BcFV6). The full-length 2945 nt genome sequence of the mitovirus, termed Botrytis cinerea mitovirus 10 (BcMV10), shares a 54% sequence identity with Fusarium boothii mitovirus 1 (FbMV1), and clusters with fungus mitoviruses, plant mitoviruses and plant mitochondria; hence BcMV10 is a new Mitoviridae member. The full-length 2759 nt and 2812 nt genome sequences of the other two botouliviruses, named Botrytis cinerea botoulivirus 18 and 19 (BcBoV18 and 19), share a 40% amino acid sequence identity with RNA-dependent RNA polymerase protein (RdRp), and these are new members of the Botoulivirus genus of Botourmiaviridae. Horizontal transmission analysis showed that BcBoV18, BcBoV19 and BcFV8 are not related to hypovirulence, suggesting that BcMV10 may induce hypovirulence. Intriguingly, a partial BcMV10 sequence was detected in cucumber plants inoculated with SZ-2-3y mycelium or pXT1/BcMV10 agrobacterium. In conclusion, we identified a hypovirulent SZ-2-3y fungal strain from P. polyphylla, coinfected with four novel mycoviruses that could serve as potential biocontrol agents. Our findings provide evidence of cross-kingdom mycoviral sequence transmission.

Four New Members of The Family Cytophagaceae: Cryseosolum Histidinii gen. nov., sp. nov., Cryseosolum Indiensis gen. nov., sp. nov., Reichenbachia Cretensis, gen. nov., sp. nov., and Reichenbachia Soli, gen. nov., sp. nov. Isolated From Diverse Habitat

Four strains isolated, PWU4T, PWU20T, PWU5T and PWU37T were from both of soil in Germany, India and a faces sheep collected in Crete Island, respectively. Cells were Gram-negative, strictly aerobic, rod shaped, grew optimally between 28oC and 34oC, pH between 7.0 and 8.0 without the addition of NaCl. Catalase and oxidase-negative and grew on most mono- and disaccharides, a few polysaccharides and organic acid. The predominant menaquinone was MK-7. Major fatty acid was c16:1 ω7c (PWU4T and PWU20T) and c16:1 ω5c (PWU5T and PWU37T). The DNA G+C content of them were 50.2 mol %; 51.6 mol %; 39.8 mol % and 53.8 mol %, respectively. The 16S rRNA gene sequence analysis revealed that the closest relatives of them are less than 93.8% compared to Ohtaekwangia koreensis 3B-2T and Ohtaekwangia kribbensis 10AOT. It classified in two groups, where PWU4T, PWU20T shared 93.0% and PWU5T, PWU37T shared 97.5% sequence similarity. However, in both groups represent different species on the low average nucleotide identity (ANI) of their genomes, 69.7% and 83.8%, respectively. We proposed that the four strains represent four novel species of two new genera in the family Cytophagaceae. The type species of the novel genus Cryseosolum are Cryseosolum histdinii gen. nov., sp. nov. strain PWU4T (=DSM 111594T=NCCB 100798T), Cryseosolum indiensis sp. nov. strain PWU20T (=DSM 111597T=NCCB 100800T). The type species of the novel genus Reichenbachia are Reichenbachia cretensis gen. nov., sp. nov. strain PWU5T (=DSM 111596T=NCCB 100799T), Reichenbachia soli sp. nov. strain PWU37T (=DSM 111595T=NCCB 100801T).

Motivated team innovation: Impact of need for closure and epistemic authority

New members are important sources of innovative perspectives in groups. However, it can be very difficult for newcomers’ ideas to be heard. It is likely that group members with high (vs. low) levels of need for closure (NFC) are more resistant to newcomers’ innovative ideas. Moreover, when group epistemic authority (EA) is high, members should “freeze” on the group’s ideas, regardless of the newcomer’s EA. In contrast, when group EA is low, members would be expected to “seize” the ideas proposed by newcomers with high EA. Study 1 confirmed that high (vs. low) NFC group members are more resistant to newcomers’ innovative ideas. In Study 2, in high NFC groups, evidence was obtained for seizing but not freezing. In Study 3, for group members with heightened NFC, both freezing and seizing results were obtained. Findings suggest that both NFC and EA play important roles in receptivity to newcomers’ ideas.

Mongolia’s “Journey” to Join the United Nations in 1946-1961 : “Cold War” Factors and Ideological Conflicts of the Great Powers

The article explores the aspects of the interaction between the USSR and the USA on the Mongolian question within the UN during first 15 years of the Cold war. The author dwells such problems as Mongolia’s contribution to the war against Japanese militarism; the question of the involvement of Ulaanbaatar to the Korean war in 1950-1953; the arguments between Moscow and Washington concerning the package admittance of new members; the reason of the veto power exercised by the Chiang Kai-shek regime; ideological conflicts between two opposing blocs in the United Nations. The first application for Mongolia’s admission to the UN was submitted to Secretary-General Trygve Lie in a letter dated June 21, 1946, signed by Kh. Choibalsan, Prime-Minister and Minister of Foreign Affairs of the MPR. The solution of this issue, however, dragged on until 1961. During this period, the application for admission of the MPR was renewed four times - in 1948, 1955, 1956 and 1957. The Mongolian statement was considered at least 13 times in various meetings of the UN Security Council.

International Order and the Access Problem of the Defeated Hegemon: the Case of Russia in the Post-Cold War Era

It has been evident that Russia as the heir of Soviet foreign policy, could neither achieve to integrate herself into the international order, nor could the international order achieve to find a solution to Russian foreign policy identity quest. As long as Russia cannot find a stable and permanent status for herself in the world politics, her foreign policy will signify a permanent instability on the behalf of the international order. The current hegemonic international order is far from residing technical capabilities in terms of satisfying Russia’s foreign policy expectations, because it is unprecedentedly rigid in terms of allowing or refusing the incorporation of hegemonic power like Russia. While it cannot return to multipolarity, it could not set a community based international order either. Since the current international order was founded upon liberal anti-Soviet values, it entered into a lightness of exposing Russia to make clear-cut choices in her foreign policy. As much as the current international order was founded upon liberal anti-Soviet values, its demands from the new members would much higher that especially Russia would not easily adapt herself to.

Natural rodent model of viral transmission reveals biological features of virus population dynamics

Emerging viruses threaten global health, but few experimental models can characterize the virus and host factors necessary for within- and cross-species transmission. Here, we leverage a model whereby pet store mice or rats—which harbor natural rodent pathogens—are cohoused with laboratory mice. This “dirty” mouse model offers a platform for studying acute transmission of viruses between and within hosts via natural mechanisms. We identified numerous viruses and other microbial species that transmit to cohoused mice, including prospective new members of the Coronaviridae, Astroviridae, Picornaviridae, and Narnaviridae families, and uncovered pathogen interactions that promote or prevent virus transmission. We also evaluated transmission dynamics of murine astroviruses during transmission and spread within a new host. Finally, by cohousing our laboratory mice with the bedding of pet store rats, we identified cross-species transmission of a rat astrovirus. Overall, this model system allows for the analysis of transmission of natural rodent viruses and is a platform to further characterize barriers to zoonosis.

Peptoniphilus Colimassiliensis sp. nov. and Peptoniphilus Urinimassiliensis sp. nov., Two New Species Isolated From Humans

Strains Marseille-P3761 and Marseille-P3195 are representatives of two bacterial species isolated from human specimens. Strain Marseille-P3761 was isolated from the stool of a healthy volunteer, while strain Marseille-P3915 was cultivated from the urine of a kidney transplant recipient. Both strains are anaerobic Gram-positive cocci bacteria. Both are catalase-negative and oxidase-negative and grow optimally at 37°C in anaerobic conditions. They also metabolize carbohydrates such as galactose, glucose, fructose, and glycerol. The major fatty acids were hexadecanoic acid for both strains, Marseille-P3761 (38%) and Marseille-P3195 (31%). The highest DNA-DNA hybridization values of Marseille-P3761 and Marseille-P3195 strains when compared to their closest phylogenetic relatives were 52.3% and 56.4%, respectively. The morphological, biochemical, phenotypic and genomic characteristics strongly support that these strains are new members of the Peptoniphilus genus. Thus, we suggest that strains Marseille-P3761 (CSUR P3761 = CCUG71569) and Marseille-P3195 (CSUR P3195 = DSM 103468) are the type strains of two new Peptoniphilus species, for which we propose the names Peptoniphilus colimassiliensis sp. nov. and Peptoniphilus urinimassiliensis sp. nov., respectively.

Liberal Self-Determination, Discrimination, and the Right to Exclude

This chapter defends one of the building blocks of a complete liberal theory of immigration by defending an account of the state’s prima facie right to exclude that has the resources to explain what is wrong with “discriminatory” exclusion in the area of immigration—that is, exclusion on the basis of morally arbitrary features, such as sex, sexuality, and race. Like other statist accounts, the chapter appeals to a right to self-determination to justify a state’s right to exclude. But unlike these other theories, it does not appeal to the psychological harm of insult. The focus is instead on the liberal aspect of self-determination and the surprising ways in which liberal principles constrain the state’s right to both include and exclude prospective new members.

Identifying new molecular players in extracellular proteostasis

Proteostasis refers to a delicately tuned balance between the processes of protein synthesis, folding, localization, and the degradation of proteins found inside and outside cells. Our understanding of extracellular proteostasis is rather limited and largely restricted to knowledge of 11 currently established extracellular chaperones (ECs). This review will briefly outline what is known of the established ECs, before moving on to discuss experimental strategies used to identify new members of this growing family, and an examination of a group of putative new ECs identified using one of these approaches. An observation that emerges from an analysis of the expanding number of ECs is that all of these proteins are multifunctional. Strikingly, the armory of activities each possess uniquely suit them as a group to act together at sites of tissue damage, infection, and inflammation to restore homeostasis. Lastly, we highlight outstanding questions to guide future research in this field.