House dust mite-specific IgE antibodies in induced sputum are associated with sputum eosinophilia in mite-sensitive asthmatics

Abstract Background Measurements of allergen-specific IgE antibodies with different manufacturers’ assays show modest or poor agreement. This study compares analytical performance of specific IgE tests for whole allergen extracts and individual allergen components of two assay systems, IMMULITE and ImmunoCAP, using human sera as well as monoclonal antibodies. Methods Comparisons were performed for specific IgE to house dust mite (HDM, n = 44), egg white (EW, n = 36) and the allergen components rDer p 1, rDer p 2, nGal d 1, nGal d 2 and nGal d 4 in human sera and with monoclonal mouse/human chimeric IgE antibodies specific for the same allergen components. Competitive interference with IgE measurement was investigated using allergen-specific monoclonal IgG and IgG4 antibodies. Results Measurements of IgE to HDM and EW in serial dilutions of human sera revealed weaker dilution linearity with IMMULITE than with ImmunoCAP. Analysis of five different monoclonal IgE antibodies with total and specific IgE assays, expected to return similar levels, gave an average specific/total IgE ratio of 0.96 (range 0.71–1.14) with ImmunoCAP and 1.89 (range 0.76–2.85) with IMMULITE, indicating overestimation of specific IgE by IMMULITE. With the EW IgE tests of both assay systems, measurements of a chimeric anti-Gal d 2 IgE antibody were unaffected by a competing mouse IgG antibody. While the same was true for measurement of a chimeric anti-Der p 1 IgE antibody using the HDM test in ImmunoCAP, a suppression of measured concentrations by up to 42% was observed in IMMULITE. Similarly, measurement of HDM-specific IgE in human sera by ImmunoCAP was unaffected by a competing monoclonal anti-Der p 2 IgG4 antibody while IMMULITE displayed a reduction of HDM-specific IgE values by up to 30%. Conclusions In this evaluation of analytical performance of two widely used assay systems, ImmunoCAP showed higher accuracy in quantitation of specific IgE and greater resistance against competing allergen-specific non-IgE antibodies which may arise through natural or dietary exposure, or as a result of allergen immunotherapy treatment.

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IgE-AB to animals molecular allergens in patients with respiratory allergy

Rossiiskii Allergologicheskii ZHurnal ◽

10.36691/rja446 ◽

2015 ◽

Vol 12 (2) ◽

pp. 25-28

Author(s):

M A Mokronosova ◽

E A Bass ◽

A M Bala ◽

T M Zheltikova

Keyword(s):

House Dust ◽

Specific Ige ◽

Respiratory Allergy ◽

House Dust Mites ◽

Dust Mites ◽

Ige Antibodies ◽

Dust Mite ◽

Specific Ige Antibodies ◽

Cat Allergen ◽

Immunocap Isac

Background. The purpose of this study was to identify IgE-antibodies to the major and minor allergenic components of pets in the blood serum of patients with bronchial asthma and/or allergic rhinitis (BA/AR). Methods. The study included 327 patients from Moscow and St. Petersburg. All of them were detected for specific IgE-antibodies (IgE-AB) to the animal allergens by ImmunoCAP® (ThermoFisherScientific, Sweden). 105 patients wanted to detect IgE-AB to the main animal allergens molecules ImmunoCAP ISAC® (ThermoFisherScientific, Sweden). Results. In the sera of 327 patients IgE-AB to cat’s (62%) allergen dominated. IgE-antibodies to dog’s, house dust mites (D.pteronyssinus, D. farinae), mold fungi, horses and rodents allergens were found in 2,6-31 times less. IgE-AB to uteroglobin (rFel d 1), major cat allergen, was detected in 80% of patients (84/105). The detection rate of IgE-AB to other cat allergens was 4,2-8 times lower (rFel d 2 - 10%, nFel d 4 - 19%). IgE-AB to major dog allergen rCan f 1 was detected in 39% of patients (41/105). Positive level of IgE-AB to other dog allergens was detected in 2,2-4,9 times lower (rCan f 2 - 8%, nCan f 3 - 9%, rCan f 5 - 18%). Positive level of IgE-AB to lipocalin was assayed in 6% of patients, to serum albumin - in 7%. Conclusion. Prevalence of IgE-AB to cat and dog allergens was almost 3 times higher than the frequency of detection of IgE-AB to house dust mite allergens in citizens of Moscow and S. Petersburg. The frequency of IgE-AB to rFel d 1 was dominated and detected 2 and 4-11 times often than to rCan f 1 and other animal allergens consequently.

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Relationship Between House Dust Mite-Specific IgE in Sputum and Airway Hyperresponsiveness in Adult Patients with Atopic Asthma

10.1164/ajrccm-conference.2019.199.1_meetingabstracts.a1344 ◽

2019 ◽

Author(s):

H. Sano ◽

K. Tomita ◽

A. Sano ◽

O. Nishiyama ◽

T. Iwanaga ◽

...

Keyword(s):

House Dust ◽

House Dust Mite ◽

Airway Hyperresponsiveness ◽

Specific Ige ◽

Adult Patients ◽

Atopic Asthma ◽

Dust Mite

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House dust mite sensitisation and association with atopic dermatitis in Brunei

Clinical and Translational Allergy ◽

10.1186/s13601-019-0304-5 ◽

2019 ◽

Vol 9 (1) ◽

Cited By ~ 1

Author(s):

Haziq Emran ◽

Christina Siew Eng Chieng ◽

Surita Taib ◽

Anne Catherine Cunningham

Keyword(s):

Atopic Dermatitis ◽

House Dust ◽

House Dust Mite ◽

Retrospective Cohort ◽

Specific Ige ◽

Dermatophagoides Pteronyssinus ◽

Food Allergens ◽

Dust Mite ◽

Storage Mite ◽

Tropical Areas

AbstractIgE sensitisation in tropical areas is under-reported. A 2 year retrospective cohort study of allergy data specific to aero and food allergens in Brunei demonstrated that specific IgE levels to house dust mite (Dermatophagoides pteronyssinus, D.farinae, Blomia tropicalis) were highest in this population and correlated with atopic dermatitis (p < 0.001). Shrimp and peanut were the most common food allergens. A dominance of house/storage mite sensitization is seen in Brunei which is consistent with other tropical countries.

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