In situ observation of solidification behavior in undercooled Pd–Cu–Ni–P alloy by using a confocal scanning laser microscope

2001 ◽  
Vol 49 (4) ◽  
pp. 615-622 ◽  
Author(s):  
J.-H Kim ◽  
S.-G Kim ◽  
A Inoue
Author(s):  
JS Deitch ◽  
KL Smith ◽  
JW Swann ◽  
JN Turner

Neurons labeled with horseradish peroxidase and reacted with diaminobenzidine (DAB) can be imaged using a confocal scanning laser microscope (CSLM) in the reflection mode. In contrast to fluorescent markers, the DAB reaction product is thought to be stable and can be observed by both light and electron microscopy. We have investigated the sensitivity of the DAB reaction product to laser irradiation, and present the spectrophotometric properties of DAB before and after exposure in the CSLM.Pyramidal neurons in slices of rat hippocampus were injected with biocytin (a biotin-lysine complex), fixed overnight in 4% paraformaldehyde, and vibratome sectioned at 75 μm. Biocytin was detected with avidin-HRP (1:200) in 0.5% Triton X-100, incubated in DAB (0.5 mg/ml) with or without 0.04% nickel ammonium sulfate (Ni), dehydrated, and imaged in a Bio Rad MRC-500 CSLM with an argon ion laser (488 and 514 nm). Spectrophotometric measurements of the soma were made on a Zeiss microspectrophotometer, as a function of laser exposure (100-1000 scans) and staining protocol.


Nephron ◽  
1995 ◽  
Vol 70 (2) ◽  
pp. 171-179 ◽  
Author(s):  
Toru Hyodo ◽  
Ikuo Miyagawa ◽  
Akihiro Iino ◽  
Koji Ono ◽  
Tsutomu Kuomi ◽  
...  

2010 ◽  
Vol 654-656 ◽  
pp. 715-718 ◽  
Author(s):  
Itsuya Sato ◽  
Seiji Miura ◽  
Tetsuo Mohri

A commercial Mg alloy, AZ31B, has been used widely. In the texture of AZ31B sheet, each grain has its c-axis almost parallel to the sheet normal. Therefore, at the bending process of the sheet, basal slip system can not accommodate an in-plane plastic strain which is perpendicular to the c-axis of each grain. It is known that {10―,12} twin can be formed by applying an extension strain parallel to the c-axis, which is equivalent to the a-axis compression strain. So in the bending deformation of the AZ31B sheet with a texture microstructure, it is expected that {10―,12} twinning occurs. In this study, an in-situ bending test of AZ31B sheet with a texture was conducted under a confocal scanning laser microscope to observe twinning by applying compression stress along a direction almost perpendicular to the c-axis of grains. In addition, EBSD techniques were used for the analysis of crystal orientations. The process of twin development observed by the in-situ bending test can be summarized as follows; with the increase of the deformation strain, the total area of twins increases. However, it is noted that the growth of twins is apparent while the number of twins is almost constant during plastic bending deformmation. EBSD analysis suggested that twinning behavior obey Schmid’s law even in the polycrystal.


Sign in / Sign up

Export Citation Format

Share Document