Cloning and expression of a NAD+-dependent xylitol dehydrogenase gene (xdhA) of Aspergillus oryzae

2004 ◽  
Vol 97 (6) ◽  
pp. 419-422 ◽  
Author(s):  
Lien Ha Tran ◽  
Noriyuki Kitamoto ◽  
Keiichi Kawai ◽  
Kazuhiro Takamizawa ◽  
Tohru Suzuki
Keyword(s):  
Aspergillus Oryzae ◽  
Xylitol Dehydrogenase ◽  
Cloning And Expression ◽  
Dehydrogenase Gene

Cloning and expression of NAD+-dependent L-arabinitol 4-dehydrogenase gene (ladA) of Aspergillus oryzae

2005 ◽  
Vol 100 (4) ◽  
pp. 472-474 ◽  
Author(s):  
Tohru Suzuki ◽  
Lien Ha Tran ◽  
Masanori Yogo ◽  
Osamu Idota ◽  
Noriyuki Kitamoto ◽  
...  
Keyword(s):  
Aspergillus Oryzae ◽  
Cloning And Expression ◽  
Dehydrogenase Gene

Cloning and expression of an Entamoeba histolytica NADP+-dependent alcohol dehydrogenase gene

1996 ◽  
Vol 1306 (1) ◽  
pp. 23-26 ◽  
Author(s):  
Mario Alberto Rodríguez ◽  
Marzella Báez-Camargo ◽  
Dulce María Delgadillo ◽  
Esther Orozco
Keyword(s):  
Alcohol Dehydrogenase ◽  
Entamoeba Histolytica ◽  
Cloning And Expression ◽  
Dehydrogenase Gene

Two lipases from Candida antarctica: cloning and expression in Aspergillus oryzae

1995 ◽  
Vol 73 (S1) ◽  
pp. 869-875 ◽  
Author(s):  
Inge Høegh ◽  
Shamkant Patkar ◽  
Torben Halkier ◽  
Mogens T. Hansen
Keyword(s):  
Key Words ◽  
Aspergillus Oryzae ◽  
Candida Antarctica ◽  
Proteolytic Processing ◽  
Basidiomycetous Yeast ◽  
Cloning And Expression ◽  
Translation Product ◽  
Active Lipase ◽  
Expression Plasmids ◽  
Lipase Expression

The basidiomycetous yeast Candida antarctica expresses two lipases that possess widely different properties. The genes LIPA and LIPB encoding both lipases were cloned and sequenced. Both lipases were secreted efficiently from Aspergillus oryzae transformed with lipase expression plasmids. N-Glycosylation was slightly more extensive in the heterologously expressed enzymes than in those purified from C. antarctica, but the enzymatic characteristics were retained. Both enzymes are encoded as preproenzymes. Proteolytic processing of the primary translation product was efficient in A. oryzae and resulted in the same N-terminals as in C. antarctica. Modifications or deletions of the propeptide of lipase component B did not prevent efficient secretion of active lipase from A. oryzae. Alternative proteolytic processing of the modified propeptides was detected. Key words: Lipase, Candida, cloning, Aspergillus, expression, propeptide.

Cloning and Expression of a Proline Dehydrogenase Gene fromCelastrus orbiculatus

2014 ◽  
Vol 49 (6) ◽  
pp. 643
Author(s):  
Wang Cuili ◽  
Tian Haixia ◽  
Wang Jiaojiao ◽  
Qi Tiancong ◽  
Huang Huang ◽  
...  
Keyword(s):  
Cloning And Expression ◽  
Proline Dehydrogenase ◽  
Dehydrogenase Gene
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