PET/CT tracking of human muscle precursor cells and neo-vascularization for tissue engineering of skeletal muscles

2019 ◽  
Vol 18 (1) ◽  
pp. e1772
Author(s):  
D. Mohr-Haralampieva ◽  
S. Salemi ◽  
D. Eberli
Keyword(s):  
Tissue Engineering ◽  
Skeletal Muscles ◽  
Precursor Cells ◽  
Human Muscle ◽  
Muscle Precursor ◽  
Pet Ct ◽  
Muscle Precursor Cells

scholarly journals Noninvasive PET Imaging and Tracking of Engineered Human Muscle Precursor Cells for Skeletal Muscle Tissue Engineering

2016 ◽  
Vol 57 (9) ◽  
pp. 1467-1473 ◽  
Author(s):  
D. Haralampieva ◽  
T. Betzel ◽  
I. Dinulovic ◽  
S. Salemi ◽  
M. Stoelting ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Tissue Engineering ◽  
Muscle Tissue ◽  
Pet Imaging ◽  
Precursor Cells ◽  
Human Muscle ◽  
Skeletal Muscle Tissue ◽  
Muscle Precursor ◽  
Muscle Precursor Cells

A role for inflammatory mediators in the modulation of the neurotrophin receptor p75NTR on human muscle precursor cells

2012 ◽  
Vol 243 (1-2) ◽  
pp. 100-102 ◽  
Author(s):  
Emanuela Colombo ◽  
Stefania Romaggi ◽  
Marina Mora ◽  
Lucia Morandi ◽  
Cinthia Farina
Keyword(s):  
Inflammatory Mediators ◽  
Precursor Cells ◽  
Human Muscle ◽  
Neurotrophin Receptor ◽  
Muscle Precursor ◽  
Muscle Precursor Cells

Human muscle precursor cells give rise to functional satellite cells in vivo

2007 ◽  
Vol 17 (8) ◽  
pp. 631-638 ◽  
Author(s):  
Janine Ehrhardt ◽  
Karima Brimah ◽  
Carl Adkin ◽  
Terence Partridge ◽  
Jennifer Morgan
Keyword(s):  
Satellite Cells ◽  
Precursor Cells ◽  
Human Muscle ◽  
Muscle Precursor ◽  
Muscle Precursor Cells

scholarly journals Aldehyde dehydrogenase activity promotes survival of human muscle precursor cells

2009 ◽  
Vol 15 (1) ◽  
pp. 119-133 ◽  
Author(s):  
Elise Jean ◽  
Dalila Laoudj-Chenivesse ◽  
Cécile Notarnicola ◽  
Karl Rouger ◽  
Nicolas Serratrice ◽  
...  
Keyword(s):  
Dehydrogenase Activity ◽  
Aldehyde Dehydrogenase ◽  
Precursor Cells ◽  
Human Muscle ◽  
Muscle Precursor ◽  
Muscle Precursor Cells ◽  
Aldehyde Dehydrogenase Activity

scholarly journals Evidence for a myogenic stem cell that is exhausted in dystrophic muscle

2000 ◽  
Vol 113 (12) ◽  
pp. 2299-2308 ◽  
Author(s):  
L. Heslop ◽  
J.E. Morgan ◽  
T.A. Partridge
Keyword(s):  
Skeletal Muscles ◽  
Precursor Cells ◽  
Mdx Mouse ◽  
Muscle Fibres ◽  
Significant Extent ◽  
Dystrophic Muscle ◽  
Myogenic Cells ◽  
Mouse Muscle ◽  
Muscle Precursor ◽  
Muscle Precursor Cells

Injection of the myotoxin notexin, was found to induce regeneration in muscles that had been subjected to 18 Gy of radiation. This finding was unexpected as irradiation doses of this magnitude are known to block regeneration in dystrophic (mdx) mouse muscle. To investigate this phenomenon further we subjected mdx and normal (C57Bl/10) muscle to irradiation and notexin treatment and analysed them in two ways. First by counting the number of newly regenerated myofibres expressing developmental myosin in cryosections of damaged muscles. Second, by isolating single myofibres from treated muscles and counting the number of muscle precursor cells issuing from these over 2 day and 5 day periods. After irradiation neither normal nor dystrophic muscles regenerate to any significant extent. Moreover, single myofibres cultured from such muscles produce very few muscle precursor cells and these undergo little or no proliferation. However, when irradiated normal and mdx muscles were subsequently treated with notexin, regeneration was observed. In addition, some of the single myofibres produced rapidly proliferative muscle precursor cells when cultured. This occurred more frequently, and the myogenic cells proliferated more extensively, with fibres cultured from normal compared with dystrophic muscles. Even after 25 Gy, notexin induced some regeneration but no proliferative myogenic cells remained associated with the muscle fibres. Thus, skeletal muscles contain a number of functionally distinct populations of myogenic cells. Most are radiation sensitive. However, some survive 18 Gy as proliferative myogenic cells that can be evoked by extreme conditions of muscle damage; this population is markedly diminished in muscles of the mdx mouse. A small third population survives 25 Gy and forms muscle but not proliferative myogenic cells.

1071 Muscle regeneration and tracking of human muscle precursor cells by MRI in vivo

2012 ◽  
Vol 11 (1) ◽  
pp. e1071-e1071a
Author(s):  
F. Azzabi ◽  
V. Jovaisaite ◽  
J. Njiwa ◽  
A. Boss ◽  
R. Rudin ◽  
...  
Keyword(s):  
Muscle Regeneration ◽  
Precursor Cells ◽  
Human Muscle ◽  
Muscle Precursor ◽  
Muscle Precursor Cells

258 CULTURING HUMAN MUSCLE PRECURSOR CELLS (MPCS) WITH XENO-FREE MEDIUM: GMP AND CLINICAL APPLICATION PREPARATION

2013 ◽  
Vol 189 (4S) ◽  
Author(s):  
Fahd Azzabi ◽  
Souzan Salemi ◽  
Ria Tauscher ◽  
Katharina Schallmoser ◽  
Dirk Strunk ◽  
...  
Keyword(s):  
Clinical Application ◽  
Precursor Cells ◽  
Human Muscle ◽  
Free Medium ◽  
Muscle Precursor ◽  
Muscle Precursor Cells

Human Muscle Precursor Cells Form Human-Derived Myofibers in Skeletal Muscles of Nonhuman Primates: A Potential New Preclinical Setting to Test Myogenic Cells of Human Origin for Cell Therapy of Myopathies

2020 ◽  
Vol 79 (12) ◽  
pp. 1265-1275
Author(s):  
Daniel Skuk ◽  
Jacques P Tremblay
Keyword(s):  
Muscle Regeneration ◽  
De Novo ◽  
Lymphocytic Infiltration ◽  
Precursor Cells ◽  
Human Muscle ◽  
Myogenic Cells ◽  
Cell Therapies ◽  
Muscle Precursor ◽  
Muscle Precursor Cells

Abstract This study aimed to verify if human myogenic cells could participate in muscle regeneration in macaques. This experimental setting would grant researchers a model that could better evaluate the effects of cell therapies in myopathies with a better translation to human patients. Human muscle precursor cells (MPCs) were cultured in vitro and transduced with ß-galactosidase. The cells were subsequently injected into 1-cm3 muscle regions of 6 macaques immunosuppressed with tacrolimus and dexamethasone. Allogeneic ß-galactosidase+ MPCs were injected in other regions as positive controls. Some cell-grafted regions were electroporated to induce extensive muscle regeneration. MPC-grafted regions were sampled 1 month later and analyzed by histology. There were ß-galactosidase+ myofibers in both the regions grafted with human and macaque MPCs. Electroporation increased the engraftment of human MPCs in the same way as in macaque allografts. The histological analysis (hematoxylin and eosin, CD8, and CD4 immunodetection) demonstrated an absence of cellular rejection in most MPC-grafted regions, as well as minimal lymphocytic infiltration in the regions transplanted with human MPCs in the individual with the lowest tacrolimus levels. Circulating de novo anti-donor antibodies were not detected. In conclusion, we report the successful engraftment of human myogenic cells in macaques, which was possible using tacrolimus-based immunosuppression.

scholarly journals Heterogeneity among muscle precursor cells in adult skeletal muscles with differing regenerative capacities

1998 ◽  
Vol 212 (4) ◽  
pp. 495-508 ◽  
Author(s):  
Grace K. Pavlath ◽  
Deepa Thaloor ◽  
Thomas A. Rando ◽  
Monica Cheong ◽  
Arthur W. English ◽  
...  
Keyword(s):  
Skeletal Muscles ◽  
Precursor Cells ◽  
Muscle Precursor ◽  
Muscle Precursor Cells
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