scholarly journals H019 Clonal analysis of the origin of the ventricular conduction system

2009 ◽  
Vol 102 ◽  
pp. S78
Author(s):  
L. Miquerol ◽  
N. Moreno ◽  
L. Dupays ◽  
S. Meilhac ◽  
M. Buckingham ◽  
...  
Keyword(s):  
Clonal Analysis ◽  
Conduction System ◽  
Ventricular Conduction

scholarly journals Nkx2-5 defines distinct scaffold and recruitment phases during formation of the murine cardiac Purkinje fiber network

2020 ◽  
Vol 11 (1) ◽  
Author(s):  
Caroline Choquet ◽  
Robert G. Kelly ◽  
Lucile Miquerol
Keyword(s):  
Transcription Factor ◽  
Electrical Activity ◽  
Cell Fate ◽  
Clonal Analysis ◽  
Conduction System ◽  
Purkinje Fiber ◽  
Fate Mapping ◽  
Fiber Network ◽  
Apply Genetic ◽  
Ventricular Conduction

Abstract The ventricular conduction system coordinates heartbeats by rapid propagation of electrical activity through the Purkinje fiber (PF) network. PFs share common progenitors with contractile cardiomyocytes, yet the mechanisms of segregation and network morphogenesis are poorly understood. Here, we apply genetic fate mapping and temporal clonal analysis to identify murine cardiomyocytes committed to the PF lineage as early as E7.5. We find that a polyclonal PF network emerges by progressive recruitment of conductive precursors to this scaffold from a pool of bipotent progenitors. At late fetal stages, the segregation of conductive cells increases during a phase of rapid recruitment to build the definitive PF network through a non-cell autonomous mechanism. We also show that PF differentiation is impaired in Nkx2-5 haploinsufficient embryos leading to failure to extend the scaffold. In particular, late fetal recruitment fails, resulting in PF hypoplasia and persistence of bipotent progenitors. Our results identify how transcription factor dosage regulates cell fate divergence during distinct phases of PF network morphogenesis.

scholarly journals Nkx2-5 defines distinct scaffold and recruitment phases during formation of the cardiac Purkinje fiber network

2020 ◽  
Author(s):  
Caroline Choquet ◽  
Robert G. Kelly ◽  
Lucile Miquerol
Keyword(s):  
Transcription Factor ◽  
Electrical Activity ◽  
Cell Fate ◽  
Clonal Analysis ◽  
Conduction System ◽  
Purkinje Fiber ◽  
Fate Mapping ◽  
Fiber Network ◽  
Apply Genetic ◽  
Ventricular Conduction

AbstractThe ventricular conduction system coordinates heartbeats by rapid propagation of electrical activity through the Purkinje fiber (PF) network. PFs share common progenitors with contractile cardiomyocytes, yet the mechanisms of segregation and network morphogenesis are poorly understood. In this study, we apply genetic fate mapping and temporal clonal analysis to identify cardiomyocytes committed to the PF lineage as early as E7.5. We find that a polyclonal PF network emerges by progressive recruitment of conductive precursors to this scaffold from a pool of bipotent progenitors. At late fetal stages, the segregation of conductive cells increases during a phase of rapid recruitment to build the definitive PF network through a non-cell autonomous mechanism. We also show that PF differentiation is impaired in Nkx2-5 haploinsufficient embryos leading to failure to extend the scaffold. In particular, late fetal recruitment fails, resulting in PF hypoplasia and persistence of bipotent progenitors. Our results identify how transcription factor dosage regulates cell fate divergence during distinct phases of PF network morphogenesis.

scholarly journals Irx3 is required for postnatal maturation of the mouse ventricular conduction system

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Kyoung-Han Kim ◽  
Anna Rosen ◽  
Samer M. I. Hussein ◽  
Vijitha Puviindran ◽  
Adam S. Korogyi ◽  
...  
Keyword(s):  
Conduction System ◽  
Postnatal Maturation ◽  
Ventricular Conduction

scholarly journals The effect of connexin40 deficiency on ventricular conduction system function during development

2012 ◽  
Vol 95 (4) ◽  
pp. 469-479 ◽  
Author(s):  
Barbora Sankova ◽  
Jiri Benes ◽  
Eliska Krejci ◽  
Laurent Dupays ◽  
Magali Theveniau-Ruissy ◽  
...  
Keyword(s):  
Conduction System ◽  
System Function ◽  
Ventricular Conduction

scholarly journals Pathological study on the atrio-ventricular conduction system in 12 cases of left axis deviation

1984 ◽  
Vol 4 (1) ◽  
pp. 25-33
Author(s):  
Takashi Hiraga ◽  
Yutaka Nakaya ◽  
Toshiharu Niki ◽  
Hiroyoshi Mori ◽  
Kazuo Hizawa
Keyword(s):  
Conduction System ◽  
Axis Deviation ◽  
Pathological Study ◽  
Ventricular Conduction

scholarly journals Development of the Ventricular Conduction System of the Crocodilian Heart

2015 ◽  
Vol 29 (S1) ◽  
Author(s):  
Alena Kvasilova ◽  
Bjarke Jensen ◽  
Vincent Christoffels ◽  
David Sedmera
Keyword(s):  
Conduction System ◽  
Ventricular Conduction

scholarly journals Specialized impulse conduction pathway in the alligator heart

eLife ◽  
2018 ◽  
Vol 7 ◽  
Author(s):  
Bjarke Jensen ◽  
Bastiaan J Boukens ◽  
Dane A Crossley ◽  
Justin Conner ◽  
Rajiv A Mohan ◽  
...  
Keyword(s):  
Conduction System ◽  
Atrioventricular Conduction ◽  
Alligator Mississippiensis ◽  
Ventricular Septum ◽  
Heart Rates ◽  
Conduction Pathway ◽  
Atrioventricular Bundle ◽  
Rapid Activation ◽  
Ventricular Conduction ◽  
Purkinje Network

Mammals and birds have a specialized cardiac atrioventricular conduction system enabling rapid activation of both ventricles. This system may have evolved together with high heart rates to support their endothermic state (warm-bloodedness) and is seemingly lacking in ectothermic vertebrates from which first mammals then birds independently evolved. Here, we studied the conduction system in crocodiles (Alligator mississippiensis), the only ectothermic vertebrates with a full ventricular septum. We identified homologues of mammalian conduction system markers (Tbx3-Tbx5, Scn5a, Gja5, Nppa-Nppb) and show the presence of a functional atrioventricular bundle. The ventricular Purkinje network, however, was absent and slow ventricular conduction relied on trabecular myocardium, as it does in other ectothermic vertebrates. We propose the evolution of the atrioventricular bundle followed full ventricular septum formation prior to the development of high heart rates and endothermy. In contrast, the evolution of the ventricular Purkinje network is strongly associated with high heart rates and endothermy.

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