Specific primers design based on the superoxide dismutase b gene for Trypanosoma cruzi as a screening tool: Validation method using strains from Colombia classified according to their discrete typing unit

ABSTRACT Trypanosoma cruzi shows a genetic diversity that has been associated with the variability of clinical manifestations, geographical distribution, and preferential parasite-vector interactions. In an effort to better understand this genetic variability, here, the draft genome of T. cruzi strain Ikiakarora (discrete typing unit TcIII), which has been associated with the sylvatic cycle, is reported.

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Genetic diversity of Trypanosoma cruzi in bats, and multilocus phylogenetic and phylogeographical analyses supporting Tcbat as an independent DTU (discrete typing unit)

Acta Tropica ◽

10.1016/j.actatropica.2015.07.015 ◽

2015 ◽

Vol 151 ◽

pp. 166-177 ◽

Cited By ~ 67

Author(s):

Luciana Lima ◽

Oneida Espinosa-Álvarez ◽

Paola A. Ortiz ◽

Javier A. Trejo-Varón ◽

Julio C. Carranza ◽

...

Keyword(s):

Genetic Diversity ◽

Trypanosoma Cruzi ◽

Discrete Typing Unit

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Detection of different Leishmania spp. and Trypanosoma cruzi antibodies in cats from the Yucatan Peninsula (Mexico) using an iron superoxide dismutase excreted as antigen

Comparative Immunology Microbiology and Infectious Diseases ◽

10.1016/j.cimid.2012.04.003 ◽

2012 ◽

Vol 35 (5) ◽

pp. 469-476 ◽

Cited By ~ 16

Author(s):

Silvia S. Longoni ◽

Angeles López-Cespedes ◽

Manuel Sánchez-Moreno ◽

Manuel E. Bolio-Gonzalez ◽

Carlos H. Sauri-Arceo ◽

...

Keyword(s):

Superoxide Dismutase ◽

Trypanosoma Cruzi ◽

Yucatan Peninsula ◽

Iron Superoxide Dismutase ◽

Yucatán Peninsula ◽

Leishmania Spp

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Anti-parasitic effect of novel amidines against Trypanosoma cruzi: phenotypic and in silico absorption, distribution, metabolism, excretion and toxicity analysis

Parasitology Open ◽

10.1017/pao.2017.5 ◽

2017 ◽

Vol 3 ◽

Cited By ~ 2

Author(s):

ALINE SILVA DA GAMA NEFERTITI ◽

MARCOS MEUSER BATISTA ◽

PATRÍCIA BERNARDINO DA SILVA ◽

EDUARDO CAIO TORRES-SANTOS ◽

EDEZIO F. CUNHA-JÚNIOR ◽

...

Keyword(s):

Trypanosoma Cruzi ◽

In Silico ◽

Host Cells ◽

Mammalian Host ◽

Aromatic Molecules ◽

Toxicity Analysis ◽

Parasitic Effect ◽

The Many ◽

Discrete Typing Unit

SUMMARYNew more selective and potent drugs are urgently need to treat Chagas disease (CD). Among the many synthetic compounds evaluated againstTrypanosoma cruzi, aromatic amidines (AAs) and especially arylimidamides (AIAs) have potent activity against this parasite. Presently, the effect of four mono-amidines (DB2228, DB2229, DB2292 and DB2294), four diamidines (DB2232, DB2235, DB2251 and DB2253) and one AIA (DB2255) was screenedin vitroagainst different forms (bloodstream trypomastigotes – BT and intracellular forms) and strains from discrete typing unit (DTU) I and VI ofT. cruziand their cytotoxic profile on mammalian host cells. Except for DB2253, all molecules were as active as benznidazole (Bz), resulting in 50% of reduction in the number of alive BT, with EC50ranging from 2·7 to 10·1µmafter 24 h of incubation. DB2255 was also the most potent against amastigotes (Tulahuen strain) showing similar activity to that of Bz (3µm).In silicoabsorption, distribution, metabolism, excretion and toxicity analysis demonstrated probability of human intestinal adsorption, while mutagenicity and inhibition of hERG1 were not predicted, besides giving acceptable predicted volumes of distribution. Our findings contribute for better knowledge regarding the biological effect of this class of aromatic molecules againstT. cruziaiming to identify novel promising agent for CD therapy.

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Overexpression of superoxide dismutase in Trypanosoma cruzi results in increased sensitivity to the trypanocidal agents gentian violet and benznidazole

Molecular and Biochemical Parasitology ◽

10.1016/s0166-6851(98)00127-3 ◽

1998 ◽

Vol 96 (1-2) ◽

pp. 167-176 ◽

Cited By ~ 32

Author(s):

Nigel J. Temperton ◽

Shane R. Wilkinson ◽

David J. Meyer ◽

John M. Kelly

Keyword(s):

Superoxide Dismutase ◽

Trypanosoma Cruzi ◽

Gentian Violet ◽

Increased Sensitivity

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Fluctuations in Trypanosoma cruzi discrete typing unit composition in two naturally infected triatomines: Mepraia gajardoi and M. spinolai after laboratory feeding

Acta Tropica ◽

10.1016/j.actatropica.2016.04.008 ◽

2016 ◽

Vol 160 ◽

pp. 9-14 ◽

Cited By ~ 6

Author(s):

Camila Egaña ◽

Raquel Pinto ◽

Fernanda Vergara ◽

Sylvia Ortiz ◽

Ricardo Campos ◽

...

Keyword(s):

Trypanosoma Cruzi ◽

Discrete Typing Unit

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Azotobacter chroococcum does not contain sodA or its gene product Mn-superoxide dismutase

Canadian Journal of Microbiology ◽

10.1139/w02-003 ◽

2002 ◽

Vol 48 (2) ◽

pp. 183-187 ◽

Cited By ~ 3

Author(s):

Jane M Caldwell ◽

Hosni M Hassan

Keyword(s):

Superoxide Dismutase ◽

Genomic Dna ◽

Ammonium Acetate ◽

Azotobacter Vinelandii ◽

Polymerase Chain Reaction Analysis ◽

Azotobacter Chroococcum ◽

Chain Reaction ◽

Specific Primers ◽

Its Gene ◽

Polymerase Chain

Azotobacter chroococcum and Azotobacter vinelandii grown in Burk medium with 1% mannitol (BM) or in BM supplemented with 2.2 mg/mL ammonium acetate (BM+N) were found to have only iron-containing and CuZn-containing superoxide dismutase. Furthermore, genomic DNA from A. chroococcum and A. vinelandii were subjected to polymerase chain reaction analysis using sodA- and sodB-specific primers and yielded only a sodB product. These results dispute the assertion by Buchanan and Lees (Can. J. Microbiol. 26: 441447, 1980) that A. chroococcum contains Mn-superoxide dismutase.Key words: FeSOD, Cu-ZnSOD, MnSOD, Azotobacter chroococcum, Azotobacter vinelandii.

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Sequence of Trypanosoma cruzi reference strain SC43 nuclear genome and kinetoplast maxicircle confirms a strong genetic structure among closely related parasite discrete typing units

Genome ◽

10.1139/gen-2020-0092 ◽

2020 ◽

pp. 1-7

Author(s):

James DeCuir ◽

Weihong Tu ◽

Eric Dumonteil ◽

Claudia Herrera

Keyword(s):

Genetic Diversity ◽

Trypanosoma Cruzi ◽

Reference Strain ◽

Nuclear Genome ◽

Neglected Tropical Disease ◽

Hybrid Strain ◽

Vector Borne ◽

Discrete Typing Unit ◽

Limited Sequence ◽

Related Parasite

Chagas disease is a zoonotic, parasitic, vector-borne neglected tropical disease that affects the lives of over 6 million people throughout the Americas. Trypanosoma cruzi, the causative agent, presents extensive genetic diversity. Here we report the genome sequence of reference strain SC43cl1, a hybrid strain belonging to the TcV discrete typing unit (DTU). The assembled diploid genome was 79 Mbp in size, divided into 1236 contigs with an average coverage reaching 180×. There was extensive synteny of SC43cl1 genome with closely related TcV and TcVI genomes, with limited sequence rearrangements. TcVI genomes included several expansions not present in TcV strains. Comparative analysis of both nuclear and kinetoplast sequences clearly separated TcV from TcVI strains, which strongly supports the current DTU classification.

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