scholarly journals Exchange between the ribosomal RNA genes of X and Y chromosomes in Drosophila melanogaster males

1981 ◽  
Vol 38 (1) ◽  
pp. 1-7 ◽  
Author(s):  
R. H. Maddern
Keyword(s):  
Drosophila Melanogaster ◽  
Ribosomal Rna ◽  
Ribosomal Rna Genes ◽  
Rrna Genes ◽  
Y Chromosomes ◽  
A Site ◽  
Rna Genes ◽  
Uniform Polarity

SUMMARYThe genes coding for the 18s and 28s ribosomal RNA (rRNA) are present on both the X and Y chromosomes of D. melanogaster at a site known as the bobbed locus. Exchange was observed in males between a normally orientated X and Y chromosome (Dp(1; 1) scv1 and BSY y31d) with a frequency of 0·079%. One-quarter (7 in 27) of these exchange products between two + chromosomes which both carried sufficient rRNA genes for a bb+ phenotype exhibited a bb phenotype. Evidence is presented that one-half, and possibly all, of the exchanges involved the repetitive bb genes. These results together with those reported by Palumbo, Caizzi & Ritossa (1973) imply that the repeated bb genes of either (or both) the X or Y chromosome are not arranged with uniform polarity and, further, that spermatogonial exchange between the X and Y chromosomes may be restricted to the bb loci.

scholarly journals Position effect suppression of ribosomal RNA genes in Drosophila melanogaster: non-autonomous in gynandromorphs?

1976 ◽  
Vol 28 (1) ◽  
pp. 89-91 ◽  
Author(s):  
Jagdeesh Pyati
Keyword(s):  
Drosophila Melanogaster ◽  
Ribosomal Rna ◽  
Position Effect ◽  
Preliminary Evidence ◽  
Genetic Evidence ◽  
Ribosomal Rna Genes ◽  
Rrna Genes ◽  
Rna Genes

SUMMARYBaker (1971) presented genetic evidence that the inviability of In(1)scL8/O and scS1/O males in Drosophila melanogaster is due to position effect suppression of ribosomal RNA cistrons. Although scL8/O and scS1/O males are inviable, scL8/scL8 and scS1/scS1 females are viable. We therefore asked the following question: Is scL8/O or scS1/O viable when part of a gynandromorph? In other words, is position effect suppression of rRNA genes autonomous or non-autonomous in gynandromorphs? In this paper preliminary evidence is presented which suggests that position effect suppression of rRNA cistrons is non-autonomous. The evidence is that scLS/ or scS1/O (male) parts of gynandromorphs are not only viable but normal in appearance.

scholarly journals Rex-induced recombination implies bipolar organization of the ribosomal RNA genes of Drosophila melanogaster.

Genetics ◽  
1988 ◽  
Vol 120 (4) ◽  
pp. 1053-1059
Author(s):  
L G Robbins ◽  
E E Swanson
Keyword(s):  
Drosophila Melanogaster ◽  
Ribosomal Rna ◽  
Mitotic Recombination ◽  
Ribosomal Rna Genes ◽  
The Other ◽  
Other Orientation ◽  
Rna Genes ◽  
Nucleolus Organizers

Abstract Rex-induced mitotic recombination was used to determine whether nucleolus organizers can pair in both inverted and noninverted orientations. Two target chromosomes, each duplicated for the rDNA region, were exposed to maternal Rex activity. Recombination in one orientation should yield deletion of the material between the two nucleolus organizers, recombination in the other orientation should yield inversion of the same material. Both products were recovered from both target chromosomes. The generality of using Rex-mediated recombination for analysis of the rDNA is considered.

Basonuclin is associated with the ribosomal RNA genes on human keratinocyte mitotic chromosomes

1999 ◽  
Vol 112 (18) ◽  
pp. 3039-3047 ◽  
Author(s):  
H. Tseng ◽  
J.A. Biegel ◽  
R.S. Brown
Keyword(s):  
Ribosomal Rna ◽  
Zinc Fingers ◽  
Hair Follicles ◽  
Ribosomal Rna Genes ◽  
Rrna Genes ◽  
Control Element ◽  
Rrna Gene ◽  
Metaphase Chromosomes ◽  
Acrocentric Chromosomes ◽  
Rna Genes

Basonuclin is a zinc finger protein mainly expressed in keratinocytes of the basal layer of epidermis and the outer root sheath of hair follicles. It is also found in abundance in the germ cells of testis and ovary. In cultured keratinocytes, basonuclin is associated with chromatin in all phases of the cell cycle, including mitosis. By immunocytochemical methods, we demonstrate here that in mitosis basonuclin is associated with the short arms of the acrocentric chromosomes and with other loci on many metaphase chromosomes of human keratinocytes. Using the evolutionarily highly conserved N-terminal pair of zinc fingers in an electrophoresis mobility shift assay, we demonstrate that the DNA target sequences of basonuclin on the acrocentric chromosomes are likely to be within the promoter region of the 45S rRNA gene transcription unit. DNase I footprinting shows that basonuclin zinc fingers interact with the upstream control element of this promoter, which is necessary for the high level of transcription of the rRNA genes. This result suggests that basonuclin may be a tissue-specific transcription factor for the ribosomal RNA genes.

Ribosomal RNA genes of Drosophila melanogaster have a novel chromatin structure

1984 ◽  
Vol 175 (2) ◽  
pp. 113-130 ◽  
Author(s):  
Andor Udvardy ◽  
Christos Louis ◽  
Stella Han ◽  
Paul Schedl
Keyword(s):  
Drosophila Melanogaster ◽  
Ribosomal Rna ◽  
Chromatin Structure ◽  
Ribosomal Rna Genes ◽  
Rna Genes

scholarly journals Suppression of ribosomal RNA genes in Drosophila melanogaster

1984 ◽  
Vol 44 (3) ◽  
pp. 351-357
Author(s):  
K. E. Kalumuck ◽  
J. D. Procunier
Keyword(s):  
Drosophila Melanogaster ◽  
Ribosomal Rna ◽  
Direct Relationship ◽  
Pupal Stage ◽  
Ribosomal Rna Genes ◽  
Repeat Type ◽  
Repeat Number ◽  
Rna Genes

SUMMARYThe rDNA of five Y chromosome mutants was examined with respect to their insert free (In−) repeat type multiplicity. The In− repeat number of each mutant was correlated with its hemizygous bobbed phenotype and additivity with an X NO bobbed (bb) mutant. Four of these mutants showed a direct relationship between their In− frequency, hemizygous bb phenotype and additivity tests. A fifth mutant, bb1–4, had a sufficient number of In− repeats to ensure viability to the late pupal stage and show additivity; however, the In− repeats genetically behaved as a complete rDNA deletion. Possible mechanisms resulting in the suppression of the bb1–4 In− repeats are discussed.

Detection of ribosomal RNA genes in soybean, Glycine max (L.) Merr., by in situ hybridization

Genome ◽  
1989 ◽  
Vol 32 (6) ◽  
pp. 1091-1095 ◽  
Author(s):  
Halina Skorupska ◽  
Marc C. Albertsen ◽  
Kim D. Langholz ◽  
Reid G. Palmer
Keyword(s):  
In Situ Hybridization ◽  
Glycine Max ◽  
Ribosomal Rna ◽  
Ribosomal Rna Genes ◽  
Rrna Genes ◽  
Single Pair ◽  
Glycine Max L ◽  
Labeled Probe ◽  
Rna Genes

A biotinylated maize rRNA probe was hybridized to soybean nuclei. Hybridization was detected by using a streptavidin horseradish peroxidase biotin system. The procedure used enabled detection of heterologous complementary 18S and 25S rRNA coding genes in soybean. In diploid cultivars 'Hark' and 'Lincoln' a single pair of satellited chromosomes was present and two binding sites were detected at interphase. In plants trisomic for the satellited chromosome, three sites were observed, and in tetraploid nuclei, four sites were seen. The in situ hybridization results indicated that, for ribosomal RNA genes, Glycine max behaves as a diploid. We discuss the possibility of loss of a pair of satellited chromosomes in the evolution of soybean.Key words: biotin-labeled probe, rRNA genes, ploidy, Glycine max.

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