scholarly journals The Hermes transposable element from the house fly, Musca domestica, is a short inverted repeat-type element of the hobo, Ac, and Tam3 (hAT) element family

1994 ◽  
Vol 64 (2) ◽  
pp. 87-97 ◽  
Author(s):  
William D. Warren ◽  
Peter W. Atkinson ◽  
David A. O'Brochta
Keyword(s):  
Zea Mays ◽  
Transposable Element ◽  
Musca Domestica ◽  
House Fly ◽  
Inverse Pcr ◽  
Sequence Motif ◽  
Terminal Sequence ◽  
Repeat Type ◽  
Genetic Tool ◽  
Element System

SummaryThe genome of the house fly, Musca domestica, contains an active transposable element system, called Hermes. Using PCR and inverse PCR we amplified and sequenced overlapping segments of several Hermes elements and from these data we have constructed a 2749 bp consensus Hermes DNA sequence. Hermes termini are composed of 17 bp imperfect inverted repeats that are almost identical to the inverted terminal repeats of the hobo element of Drosophila melanogaster. Full length Hermes elements contain a single long ORF capable of encoding a protein of 612 amino acids which is 55% identical to the amino acid sequence of the hobo transposase. Comparison of the ends of the Hermes and hobo elements to those of the Ac element of Zea mays, and the Tam3 element of Antirrhinum majus, as well as several other plant and insect elements, revealed a conserved terminal sequence motif. Thus Hermes is clearly a member of the hobo, Ac and Tam3 (hAT) transposable element family, other members of which include the Tagl element from Arabidopsis thaliana and the Bg element from Zea mays. The evolution of this class of transposable elements and the potential utility of Hermes as a genetic tool in M. domestica and related species are discussed.

Genetic and molecular analysis of the Enhancer (En) transposable element system of Zea mays

1985 ◽  
Vol 4 (1) ◽  
pp. 17-23 ◽  
Author(s):  
Andy Pereira ◽  
Zsuzsanna Schwarz-Sommer ◽  
Alfons Gierl ◽  
Isolde Bertram ◽  
Peter A. Peterson ◽  
...  
Keyword(s):  
Zea Mays ◽  
Transposable Element ◽  
Molecular Analysis ◽  
Element System

scholarly journals Transposable Elements in Maize the?Activator-Dissociation (Ac-Ds) System

1984 ◽  
Vol 37 (6) ◽  
pp. 307 ◽  
Author(s):  
E A Howard ◽  
E S Dennis
Keyword(s):  
Zea Mays ◽  
Transposable Elements ◽  
Transposable Element ◽  
Chromosomal Location ◽  
Lower Eukaryotes ◽  
Element System ◽  
Controlling Element ◽  
Series Of Experiments ◽  
Higher Eukaryotes ◽  
Living Organisms

Although unstable mutants in maize (Zea mays) were described as early as 1914 (Emerson 1914, 1917, 1929; Rhoades 1936, 1938), the first explanation of such mutants in terms of transposable DNA was provided by Barbara McClintock's elegant series of experiments on the activator-dissociation (Ac-Ds) controlling-element system of maize (McClintock 1947,1948, 1951). McClintock demonstrated genetically thatAc and Ds were short regions of DNA which could move (transpose) from one chromosomal location to another. McClintock also established that Ds could transpose only in response to the action of Ac (i.e. both elements were required in the same nucleus for Ds transposition), and that Ac could transpose autonomously (i.e. in the absence of Ds). A total of eight transposable element systems have been recognized in maize, the best characterized of which are Ac(Mp)Ds, Spm and Robertson's mutator (reviewed in Fedoroff 1983; Nevers et al. 1984). All but Robertson's mutator occur as two-element systems, similar to Ac-Ds. Transposable elements have now been shown to be widespread in living organisms-occurring in prokaryotes and lower eukaryotes as well as other higher eukaryotes, including animals.

Structure and Function of the En/Spm Transposable Element System of Zea Mays: Identification of the Suppressor Component of En

1988 ◽  
pp. 115-119 ◽  
Author(s):  
Alfons Gierl ◽  
Heinrich Cuypers ◽  
Stephanie Lütticke ◽  
Andy Pereira ◽  
Zsuzsanna Schwarz-Sommer ◽  
...  
Keyword(s):  
Zea Mays ◽  
Transposable Element ◽  
Structure And Function ◽  
Element System ◽  
And Function

scholarly journals The defective En-I102 element encodes a product reducing the mutability of the En/Spm transposable element system of Zea mays

1988 ◽  
Vol 7 (10) ◽  
pp. 2953-2960 ◽  
Author(s):  
Heinrich Cuypers ◽  
Sudhansu Dash ◽  
Peter A. Peterson ◽  
Heinz Saedler ◽  
Alfons Gierl
Keyword(s):  
Zea Mays ◽  
Transposable Element ◽  
Element System

scholarly journals Molecular analysis of the En/Spm transposable element system of Zea mays

1986 ◽  
Vol 5 (5) ◽  
pp. 835-841 ◽  
Author(s):  
Andy Pereira ◽  
Heinrich Cuypers ◽  
Alfons Gierl ◽  
Zsuzsanna Schwarz-Sommer ◽  
Heinz Saedler
Keyword(s):  
Zea Mays ◽  
Transposable Element ◽  
Molecular Analysis ◽  
Element System

scholarly journals Molecular interactions between the components of the En-I transposable element system of Zea mays

1985 ◽  
Vol 4 (3) ◽  
pp. 579-583 ◽  
Author(s):  
Alfons Gierl ◽  
Zsuzsanna Schwarz-Sommer ◽  
Heinz Saedler
Keyword(s):  
Zea Mays ◽  
Transposable Element ◽  
Molecular Interactions ◽  
Element System

Molecular analysis of the Ubiquitous (Uq) transposable element system of Zea mays

1991 ◽  
Vol 230 (1-2) ◽  
pp. 201-208 ◽  
Author(s):  
A. G. Pisabarro ◽  
W. F. Martin ◽  
P. A. Peterson ◽  
H. Saedler ◽  
A. Gierl
Keyword(s):  
Zea Mays ◽  
Transposable Element ◽  
Molecular Analysis ◽  
Element System

scholarly journals Hermes, a Functional Non-Drosophilid Insect Gene Vector From Musca domestica

Genetics ◽  
1996 ◽  
Vol 142 (3) ◽  
pp. 907-914 ◽  
Author(s):  
David A O'Brochta ◽  
William D Warren ◽  
Kenneth J Saville ◽  
Peter W Atkinson
Keyword(s):  
Musca Domestica ◽  
Southern Blotting ◽  
House Fly ◽  
Transformation Rate ◽  
Gene Vector ◽  
Repeat Type ◽  
Large Clusters ◽  
Transgenic Insects ◽  
Recombination Assay

Abstract Hermes is a short inverted repeat-type transposable element from the house fly, Musca domestica. Using an extra-chromosomal transpositional recombination assay, we show that Hermes elements can accurately transpose in M. domestica embryos. To test the ability of Hermes to function in species distantly related to M. domestica we used a nonautonomous Hermes element containing the Drosophila melanogaster white (w  +) gene and created D. melanogaster germline transformants. Transgenic G1, insects were recovered from 34.6% of the fertile Go adults developing from microinjected w  − embryos. This transformation rate is comparable with that observed using P or hobo vectors in D. melanogaster, however, many instances of multiple-element insertions and large clusters were observed. Genetic mapping, Southern blotting, polytene chromosome in situ hybridization and DNA sequence analyses confirmed that Hermes elements were chromosomally integrated in transgenic insects. Our data demonstrate that Hermes elements transpose at high rates in D. melanogaster and may be an effective gene vector and gene-tagging agent in this species and distantly related species of medical and agricultural importance.

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