A new technique using cultured epithelial sheets for the management of epistaxis associated with hereditary haemorrhagic telangiectasia

1993 ◽  
Vol 107 (6) ◽  
pp. 510-513 ◽  
Author(s):  
Catherine M. Milton ◽  
J. C. Shotton ◽  
D. J. Premachandran ◽  
Barbara M. Woodward ◽  
J. W. Fabre ◽  
...  
Keyword(s):  
Nasal Septum ◽  
Squamous Epithelium ◽  
Respiratory Epithelium ◽  
New Technique ◽  
Hereditary Haemorrhagic Telangiectasia ◽  
Buccal Epithelium ◽  
Severe Epistaxis ◽  
Stratified Squamous Epithelium ◽  
A New Technique ◽  
Epithelial Sheets

A new technique for the treatment of severe epistaxis associated with hereditary haemorrhagic telangiectasia is described. The nasal septum and inferior turbinates, surgically denuded of respiratory epithelium, were grafted using autografts of cultured epithelial sheets derived from buccal epithelium. All patients upon whom this technique has been used have shown considerable lessening in the frequency and severity of their epistaxes although two patients received grafts on two occasions, in each case approximately three months apart. It is postulated that a nasal lining of stratified squamous epithelium is likely to be more resistant to trauma than the normal respiratory type, and this is supported by the observation that bleeds very seldom occur from the oral cavity in this syndrome.

scholarly journals Total upper eyelid reconstruction by single staged malar-cheek flap

2014 ◽  
Vol 47 (01) ◽  
pp. 116-119
Author(s):  
Rajeev B. Ahuja ◽  
Pallab Chatterjee ◽  
Gaurav K. Gupta ◽  
Prabhat Shrivastava
Keyword(s):  
Surgical Outcome ◽  
Nasal Septum ◽  
Lower Eyelid ◽  
New Technique ◽  
Upper Eyelid ◽  
Eyelid Reconstruction ◽  
A New Technique ◽  
Eye Closure ◽  
Cheek Flap

ABSTRACTWe report a case of total upper eyelid reconstruction by a new technique after excision of an eyelid tumour. The eyelid was reconstructed by a horizontal, laterally based flap from just under the lower eyelid combined with a chondro-mucosal graft from the nasal septum. Surgical outcome was an excellent aesthetically reconstructed eyelid, which was mobile and properly gliding on the globe to achieve complete eye closure.

Treatment of chronic mastoiditis by grafting of mastoid cavities with autologous epithelial layers generated by in vitro culture of buccal epithelium

1991 ◽  
Vol 105 (6) ◽  
pp. 413-416 ◽  
Author(s):  
D. J. Premachandra ◽  
B. Woodward ◽  
C. M. Milton ◽  
R. J. Sergeant ◽  
J. W. Fabre
Keyword(s):  
Complete Resolution ◽  
Squamous Epithelium ◽  
Substantial Improvement ◽  
Buccal Epithelium ◽  
Topical Anaesthesia ◽  
Mucosal Biopsy ◽  
Stratified Squamous Epithelium ◽  
Epithelial Layers

AbstractAutologous cultured epithelial layers were established from biopsies from the mucosa of the cheek, a nonkeratinizing region of the oral cavity. These were grafted to the unepithelialized mastoid cavities of nine patients with chronic mastoiditis and severe otorrhoea varying from two to 30 years' duration. All procedures were performed on an out-patient basis, with no anaesthesia except for topical anaesthesia for the mucosal biopsy. In seven of the patients the grafts took well, with complete resolution of the otorrhoea for a minimum follow-up period of eight months. In one patient there was a partial take of the graft with substantial improvement in the rate of discharge. The mastoid cavities of two patients were biopsied five months after grafting, and demonstrated a stratified squamous epithelium, with keratinization of the epithelium clearly evident.

scholarly journals Microscopic Structure of the Epithelium Lining the Epiglottis in Wistar Rats

2018 ◽  
Vol 75 (1) ◽  
pp. 5
Author(s):  
Vasile RUS ◽  
Aura Ștefania PARDI ◽  
Bianca MATOSZ ◽  
Flavia RUXANDA
Keyword(s):  
Histological Examination ◽  
Wistar Rats ◽  
Squamous Epithelium ◽  
Respiratory Epithelium ◽  
Microscopic Structure ◽  
Stratified Squamous Epithelium ◽  
Keratin Layer

The aim of this study was to investigate the type of the epithelium present on the epiglottis surface, through histological examination. The epithelium lining the pharyngeal side of epiglottis is keratinized stratified squamous. The thickness of the keratin layer is different from one subject to another. Usually the keratin layer extends to the apex of the epiglottis, but in some of the animals taken into study, the keratin layer extends to the apical third of the laryngeal side of epiglottis. The epithelium lining the laryngeal side is different. In most of the epiglottises examined, the surface is lined by non-keratinized stratified squamous epithelium in the apical third. The rest of the laryngeal side of the epiglottis is lined by respiratory epithelium type. In one subject, the entire laryngeal surface is lined by a non-keratinized stratified squamous epithelium. Our study highlights that the pharyngeal side of epiglottis is lined by a keratinized stratified squamous epithelium, whereas on the laryngeal side, the epithelium in the apical third is non-keratinized stratified squamous and at the rest of the surface the epithelium is pseudostratified.

Junction of the Epithelium and Lamina Propria of the Bovine Rumen Mucosa

1967 ◽  
Vol 25 ◽  
pp. 68-69
Author(s):  
Al W. Stinson
Keyword(s):  
Osmium Tetroxide ◽  
Squamous Epithelium ◽  
Short Chain Fatty Acids ◽  
Chloride Ions ◽  
Fermentation Products ◽  
Ruminant Species ◽  
Protective Shield ◽  
Stratified Squamous Epithelium ◽  
Lead Hydroxide ◽  
Acetic Acids

The stratified squamous epithelium which lines the ruminal compartment of the bovine stomach performs at least three important functions. (1) The upper keratinized layer forms a protective shield against the rough, fibrous, constantly moving ingesta. (2) It is an organ of absorption since a number of substances are absorbed directly through the epithelium. These include short chain fatty acids, potassium, sodium and chloride ions, water, and many others. (3) The cells of the deeper layers metabolize butyric acid and to a lesser extent propionic and acetic acids which are the fermentation products of rumen digestion. Because of the functional characteristics, this epithelium is important in the digestive process of ruminant species which convert large quantities of rough, fibrous feed into energy.Tissue used in this study was obtained by biopsy through a rumen fistula from clinically healthy, yearling holstein steers. The animals had been fed a typical diet of hay and grain and the ruminal papillae were fully developed. The tissue was immediately immersed in 1% osmium tetroxide buffered to a pH of 7.4 and fixed for 2 hrs. The tissue blocks were embedded in Vestapol-W, sectioned with a Porter-Blum microtome with glass knives and stained with lead hydroxide. The sections were studied with an RCA EMU 3F electron microscope.

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