Trypanosome infections and survival in tsetse

Parasitology ◽  
1998 ◽  
Vol 116 (S1) ◽  
pp. S23-S28 ◽  
Author(s):  
I. Maudlin ◽  
S. C. Welburn ◽  
P. J. M. Milligan
Keyword(s):  
Salivary Gland ◽  
Salivary Glands ◽  
Trypanosoma Brucei ◽  
Glossina Morsitans Morsitans ◽  
Trypanosoma Congolense ◽  
Vectorial Capacity ◽  
Trypanosome Infection ◽  
Trypanosoma Brucei Rhodesiense ◽  
Glossina Morsitans ◽  
Differential Effects

SummaryThe effect of trypanosome infection on vector survival was observed in a line of Glossina morsitans morsitans selected for susceptibility to trypanosome infection. The differential effects of midgut and salivary gland infections on survival were examined by exposing flies to infection with either Trypanosoma congolense which colonizes midgut and mouthparts or Trypanosoma brucei rhodesiense which colonizes midgut and salivary glands. A comparison of the survival distributions of uninfected flies with those exposed to infection showed that salivary gland infection significantly reduces tsetse survival; midgut infection had little or no effect on the survival of tsetse. The significance of these findings is discussed in relation to the vectorial capacity of wild flies.

Rickettsia-like organisms and chitinase production in relation to transmission of trypanosomes by tsetse flies

Parasitology ◽  
1993 ◽  
Vol 107 (2) ◽  
pp. 141-145 ◽  
Author(s):  
S. C. Welburn ◽  
K. Arnold ◽  
I. Maudlin ◽  
G. W. Gooday
Keyword(s):  
Glossina Morsitans Morsitans ◽  
Tsetse Flies ◽  
Trypanosome Infection ◽  
Infection Rates ◽  
Chitinolytic Activity ◽  
Trypanosoma Brucei Rhodesiense ◽  
Glossina Morsitans ◽  
High Incidence ◽  
Chitinase Production ◽  
Related Increase

SUMMARYRickettsia-like organisms (RLO) from tsetse midguts and mosquito cell cultures showed high levels of endochitinase activity. A line of Glossina morsitans morsitans highly susceptible to midgut trypanosome infection and with high incidence of RLO infection showed significantly greater chitinolytic activity than G. austeni which had low RLO incidence and were correspondingly refractory to midgut infection. Midgut infection rates of Trypanosoma brucei rhodesiense in G. m. morsitans showed a dose-related increase when flies were fed N-acetyl-D-glucosamine (GlcNAc) in the infective meal and for 4 subsequent days. A model is proposed for susceptibility to trypanosome infection based on the generation of GlcNAc by RLO endochitinase activity in tsetse pupae inhibiting midgut lectin in teneral flies.

Serological responses in rabbits used to maintain uninfected, laboratory-reared tsetses (Glossina morsitans morsitans Westwood) (Diptera: Glossinidae)

1979 ◽  
Vol 57 (4) ◽  
pp. 705-710 ◽  
Author(s):  
K. R. Parker
Keyword(s):  
Salivary Gland ◽  
Salivary Glands ◽  
Gel Filtration ◽  
Glossina Morsitans Morsitans ◽  
Immunological Reaction ◽  
Glossina Morsitans ◽  
Anamnestic Response ◽  
Molecular Weights ◽  
Antigen Antibody

Antibodies, reacting with homogenatesof salivary glands, were produced in rabbits exposed to Glossina morsitans morsitans Westwood. Precipitating antibodies and high titres of haemagglutinating antibodies occurred in all exposed rabbits. Precipitating antibodies, identified using immunoelectrophoresis, immunodiffusion, and precipitin ring tests, developed within 11 days of exposure. As many as seven antigen–antibody precipitin arcs were identified using immunoelectrophoresis. All precipitating antigens in the salivary glands had molecular weights greater than 25 000 (determined by Sephadex gel filtration); the salivary gland anticoagulant was not shown to be antigenic. No precipitating immunological reaction occurred between rabbit sera and tsetse hindguts or midguts. Titres of sera from rabbits receiving a second exposure to tsetses, following a period of no exposure, followed an anamnestic response.

Identification of major soluble salivary gland proteins in teneral Glossina morsitans morsitans

2002 ◽  
Vol 32 (9) ◽  
pp. 1045-1053 ◽  
Author(s):  
J.D. Haddow ◽  
B. Poulis ◽  
L.R. Haines ◽  
R.H. Gooding ◽  
S. Aksoy ◽  
...  
Keyword(s):  
Salivary Gland ◽  
Glossina Morsitans Morsitans ◽  
Glossina Morsitans
Sign in / Sign up

Export Citation Format

Share Document