Application of post-embedding immunogold technique in the diagnostic evaluation of fine-needle aspirates of neuroendocrine tumors

1991 ◽  
Vol 49 ◽  
pp. 274-275
Author(s):  
M. Diocee ◽  
E. Ravinsky ◽  
G. Quinonez
Keyword(s):  
Neuroendocrine Tumor ◽  
Chromogranin A ◽  
Room Temperature ◽  
Diagnostic Feature ◽  
Electron Microscopic ◽  
Sodium Metaperiodate ◽  
Fine Needle ◽  
Fine Needle Aspirates ◽  
Transmission Electron ◽  
Diamond Knife

The diagnostic feature of a neuroendocrine tumor by transmission electron microscopy (TEM) is the presence of neurosecretory granules (NSG). However, other electron dense inclusions like lipid droplets and primary lysosomes may mimic NSC. In this study, post-embedding immunogold (PEI) technique using chromogranin A antibody has been used to confirm the ultrastructural impression of neuroendocrine tumor. This technique is contrasted with immunohistochemical results.Material for this study was obtained from 5 transabdominal fine needle aspirates of multiple hepatic masses of unknown nature, processed for light and electron microscopic examinations and immunohistochemistry. Thin gold sections were cut (Reichart) with a Diatome diamond knife and mounted on 200 mesh nickel grids. A nickel grid with section side down was placed on drops of 11% aqueous sodium metaperiodate for 30 minutes, and was then washed with distilled water. Grids were incubated in 1% albumin for 15 minutes and transferred directly to chromogranin A and incubated for 60 minutes at room temperature.

Formation of a unique glass by spark plasma sintering of a zeolite

2009 ◽  
Vol 24 (10) ◽  
pp. 3241-3245 ◽  
Author(s):  
Lianjun Wang ◽  
Wan Jiang ◽  
Lidong Chen ◽  
Zhijian Shen
Keyword(s):  
Spark Plasma Sintering ◽  
Glass Sample ◽  
Room Temperature ◽  
Vickers Microhardness ◽  
Simple Approach ◽  
Electron Microscopic ◽  
Plasma Sintering ◽  
Transmission Electron ◽  
Transmission Electron Microscopic ◽  
Spark Plasma

A simple approach, order–disorder transition (ODT), has been developed to synthesize a novel glass using ZSM-5 as starting materials. In this process, the ZSM-5 powders were pressed uniaxially in a graphite die and rapidly sintered using spark plasma sintering (SPS). High-resolution transmission electron microscopic images revealed that a few crystalline zeolite fragments were still preserved locally inside the SPS consolidated sample. Vickers microhardness and fracture toughness of this as-prepared transparent glass sample at room temperature reaches 7.3 ± 0.2 GPa and 2.0 ± 0.3MPa·m1/2, respectively. It is very interesting that these novel bulk transparent glasses exhibit ultraviolet photoluminescence (PL) properties at about ∼360 nm.

scholarly journals Atypical cytologic presentation of a histiocytic sarcoma in a Cavalier King Charles Spaniel dog

2017 ◽  
Vol 29 (4) ◽  
pp. 541-543 ◽  
Author(s):  
Lorelei L. Clarke ◽  
Lisa S. Kelly ◽  
Bridget Garner ◽  
Cathy A. Brown
Keyword(s):  
Chromogranin A ◽  
Adrenal Glands ◽  
Clinical Presentation ◽  
Mitotic Rate ◽  
Histiocytic Sarcoma ◽  
Cavalier King Charles Spaniel ◽  
Fine Needle ◽  
Fine Needle Aspirates ◽  
Mhc Ii ◽  
History Of

A Cavalier King Charles Spaniel dog was presented because of a 10-d history of progressive vomiting, inappetence, and lethargy, with mild neurologic signs. Fine-needle aspirates of splenic nodules seen on ultrasound were suggestive of a carcinoma. On autopsy, a disseminated neoplasm was present in the lung, spleen, and adrenal glands. Additionally, there was a Chiari-like malformation of the skull with corresponding syringomyelia of the cranial spinal cord. Histologically, the neoplasm was comprised of a pleomorphic population of round cells with a high mitotic rate. Cells stained positive for vimentin and CD18 and negative for cytokeratin, chromogranin A, CD3, CD20, MHC II, and CD11d, and hence the tumor was diagnosed as a histiocytic sarcoma. The cytologic presentation in this case is uncommon for this type of tumor, as was the clinical presentation.

The Effect of Cu-Macroalloying Additions to Rapidly Solidified NiAl Intermetallic Compound

2000 ◽  
Vol 646 ◽  
Author(s):  
J. Colin ◽  
B. Campillo ◽  
C. Gonzalez ◽  
O. Alvarez-Fregoso ◽  
J. A. Juarez-Islas
Keyword(s):  
Intermetallic Compound ◽  
Room Temperature ◽  
Alloy Composition ◽  
Argon Atmosphere ◽  
Solidification Processing ◽  
Electron Microscopic ◽  
Room Temperature Ductility ◽  
Transmission Electron ◽  
Transmission Electron Microscopic ◽  
Rapidly Solidified

ABSTRACTThe effects of two variables on the NiAl intermetallic compound were studied: 1) copper macroalloying additions and 2) rapid solidification processing. For that purpose, several NiCuAl alloys were vacuum induction melted and rapidly solidified by using a copper wheel, rotating at 15 m/s, under an argon atmosphere. Chemical analysis of as-rapidly solidified ribbons indicated, that four alloy compositions lie in the β-(Ni, Cu)Al field, one alloy composition lie in the boundary of the β-(Ni, Cu)Al/(Ni, Cu)2Al3 fields, one alloy composition lies in the boundary of the β-(Ni, Cu)Al/β-(Ni, Cu)Al + (Ni, Cu)3Al fields and two alloy compositions lie in the β-(Ni, Cu)Al + (Ni, Cu)3Al field. Transmission electron microscopic observations carried out in as-rapidly solidified ribbons, revealed the presence of at least three main structures: i) β-(Ni, Cu)Al, ii) β-(Ni, Cu)Al + martensite (Ni, Cu)Al and iii) (Ni, Cu)3Al + martensite (Ni, Cu)Al. Microhardness Vickers and tensile test data indicated that alloys with a β-(Ni, Cu)Al + martensite (Ni, Cu)Al microstructure have improved room temperature ductility, reaching values of elongation up to 3.28 %.

Deformation and Corrosion Behavior of a High Purity Manganese Free AZ31 Magnesium Alloy

2007 ◽  
Vol 26-28 ◽  
pp. 1283-1286 ◽  
Author(s):  
Eigo Kakutani ◽  
Masahiro Jotoku ◽  
Atsushi Yamamoto ◽  
Harushige Tsubakino
Keyword(s):  
Magnesium Alloy ◽  
Corrosion Behavior ◽  
Deformation Behavior ◽  
Room Temperature ◽  
Commercial Alloy ◽  
Electron Microscopic ◽  
Corrosion Morphology ◽  
Transmission Electron ◽  
Transmission Electron Microscopic ◽  
Cold Rolled

A low impurity magnesium alloy has bean prepared. Deformation behavior in cold-rolling and corrosion behavior of the alloy were compared with those of a commercial alloy. The specimens were cold-rolled at room temperature with reduction rates of 0~80 %. Transmission electron microscopic observations on the cold-rolled specimens were carried out. In the case of the low impurity magnesium alloy, recrystallization easily occurred. Analyses of microstructures in the deformed specimens were carried out by means of EBSP, and the recrystallization phenomena have been discussed. Another effect of lowering the impurities is to improve a corrosion resistance with changing the corrosion morphology.

scholarly journals Transmission electron microscopy of percutaneous fine needle aspirates from lung: a study of 70 cases.

Thorax ◽  
1987 ◽  
Vol 42 (4) ◽  
pp. 296-301 ◽  
Author(s):  
D C Davies ◽  
A J Russell ◽  
R Tayar ◽  
N T Cooke ◽  
M M Levene
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Fine Needle ◽  
Fine Needle Aspirates ◽  
Transmission Electron

A Reproducible Selective Stain for Cardiac Sarcoplasmic Reticulum

1974 ◽  
Vol 32 ◽  
pp. 214-215
Author(s):  
R. A. Waugh ◽  
J. R. Sommer
Keyword(s):  
Complex System ◽  
Electron Microscope ◽  
Sarcoplasmic Reticulum ◽  
Transmission Electron Microscope ◽  
Electron Microscopic ◽  
Cardiac Sarcoplasmic Reticulum ◽  
Transmission Electron

Cardiac sarcoplasmic reticulum (SR) is a complex system of intracellular tubules that, due to their small size and juxtaposition to such electron-dense structures as mitochondria and myofibrils, are often inconspicuous in conventionally prepared electron microscopic material. This study reports a method with which the SR is selectively “stained” which facilitates visualizationwith the transmission electron microscope.

Domain Formation in Synthetic Quartz

1971 ◽  
Vol 29 ◽  
pp. 156-157
Author(s):  
Joseph J. Comer
Keyword(s):  
Electron Beam ◽  
Room Temperature ◽  
Domain Formation ◽  
Synthetic Quartz ◽  
Transmission Electron ◽  
Black Spots ◽  
Diffraction Mode ◽  
Domain Boundaries ◽  
Kikuchi Lines ◽  
Straight Lines

Domains visible by transmission electron microscopy, believed to be Dauphiné inversion twins, were found in some specimens of synthetic quartz heated to 680°C and cooled to room temperature. With the electron beam close to parallel to the [0001] direction the domain boundaries appeared as straight lines normal to <100> and <410> or <510> directions. In the selected area diffraction mode, a shift of the Kikuchi lines was observed when the electron beam was made to traverse the specimen across a boundary. This shift indicates a change in orientation which accounts for the visibility of the domain by diffraction contrast when the specimen is tilted. Upon exposure to a 100 KV electron beam with a flux of 5x 1018 electrons/cm2sec the boundaries are rapidly decorated by radiation damage centers appearing as black spots. Similar crystallographio boundaries were sometimes found in unannealed (0001) quartz damaged by electrons.

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