ULTRASTRUCTURE OF PINEAL GLAND TUMOR IN WHITE RAT

Tumors of pineal gland are very rare brain lesion in the rats and other species like humans. Neoplasms of pineal gland should be included in the possible differential diagnosis list for brain tumor, This type happened when the tumor is sited in the pineal body region. Research scientific investigation of pineal glands of rats being of important from the scientific point view, Transmission electron microscopic study of old and young rats was done to study the pineal gland associated with aging, these changes characterized by presence of fat like droplet in cytoplasm. Furthermore in the terminal end pinealocytes which were synapsis like associated with the presence of core vesicles containing neurotransmitter like in consistency.

Degradation of mitochondrial structure and deficiency of complex I were associated with the transgenic CMS of rice

Background Mitochondria play a significant role in plant cytoplasmic male sterility (CMS). In our previous study, mitochondrial complex I genes, nad4, nad5, and nad7 showed polymorphisms between the transgenic CMS line M2BS and its wild type M2B. The sterility mechanism of the M2BS at cytological, physiological, biochemical, and molecular level is not clear. Results Cytological observation showed that the anthers were light yellow, fissured, invalid in KI-I2, and full of irregularly typical abortion pollen grains in M2BS. Transmission electron microscopic (TEM) observation revealed no nucleus and degraded mitochondria with obscure cristae in anther cells of M2BS. The results of staining for H2O2 presented a large number of electron dense precipitates (edp) in intercellular space of anther cells of M2BS at anthesis. Moreover, the anther respiration rate and complex I activity of M2BS were significantly lower than those of wild type M2B during pollen development. Furthermore, RNA editing results showed only nad7 presented partially edited at 534th nucleotides. The expression of nad5 and nad7 revealed significant differences between M2B and M2BS. Conclusions Our data demonstrated that mitochondrial structural degradation and complex I deficiency might be associated with transgenic CMS of rice.