scholarly journals Comparison in the immunological properties ofBorrelia burgdorferiisolates fromIxodes ricinusderived from three endemic areas in Switzerland

1994 ◽  
Vol 112 (3) ◽  
pp. 533-542 ◽  
Author(s):  
C. M. Hu ◽  
S. Leuba-Garcia ◽  
M. D. Kramer ◽  
A. Aeschlimann ◽  
L. Gern
Keyword(s):  
Monoclonal Antibody ◽  
Borrelia Burgdorferi ◽  
Lyme Borreliosis ◽  
Ixodes Ricinus ◽  
Outer Surface ◽  
Surface Proteins ◽  
Immunological Properties ◽  
Outer Surface Proteins ◽  
Sds Page ◽  
Further Development

SUMMARYBorrelia burgdorferiisolates were obtained fromIxodes ricinusfrom three sites in Switzerland. They were examined by SDS-PAGE and immunoblotting. The phenotypes, in respect of three outer surface proteins (Osp), differed between the sites of collection. In site 1, most isolates had an OspA of 31 kDa and an OspB of 34 kDa: in site 2, isolates presenting an OspA of 33 kDa dominated and in site 3, the isolates with an OspA of 32 kDa and an OspB of 35 kDa were most frequent. This distribution differed significantly. About half of the isolates from sites 1 and 3 reacted with anti-OspA monoclonal antibody H5332 compared to 29% from site 2. Site 1 isolates reacted significantly more frequently (81 %) with another anti-OspA monoclonal antibody LA-31 than isolates from site 3 (P< 0·0001). These findings have implications for the epidemiology of Lyme borreliosis, for the further development of serodiagnostic reagents and for the development of a vaccine.

scholarly journals Expression of outer surface proteins A and C of Borrelia burgdorferi in Ixodes ricinus.

1995 ◽  
Vol 33 (7) ◽  
pp. 1867-1869 ◽  
Author(s):  
V Fingerle ◽  
U Hauser ◽  
G Liegl ◽  
B Petko ◽  
V Preac-Mursic ◽  
...  
Keyword(s):  
Borrelia Burgdorferi ◽  
Ixodes Ricinus ◽  
Outer Surface ◽  
Surface Proteins ◽  
Outer Surface Proteins

scholarly journals The role of Borrelia burgdorferi outer surface proteins

2012 ◽  
Vol 66 (1) ◽  
pp. 1-19 ◽  
Author(s):  
Melisha R. Kenedy ◽  
Tiffany R. Lenhart ◽  
Darrin R. Akins
Keyword(s):  
Borrelia Burgdorferi ◽  
Outer Surface ◽  
Surface Proteins ◽  
Outer Surface Proteins

scholarly journals Complement Receptor 3 Binds the Borrelia burgdorferi Outer Surface Proteins OspA and OspB in an iC3b-Independent Manner

2005 ◽  
Vol 73 (9) ◽  
pp. 6138-6142 ◽  
Author(s):  
Rodolfo C. Garcia ◽  
Rossella Murgia ◽  
Marina Cinco
Keyword(s):  
Borrelia Burgdorferi ◽  
Outer Surface ◽  
Surface Proteins ◽  
Vertebrate Host ◽  
Target Cells ◽  
Complement Receptor ◽  
Independent Manner ◽  
Outer Surface Proteins ◽  
Direct Binding ◽  
Complement Receptor 3

ABSTRACT Persistence of borreliae within the vertebrate host depends on the fate of interactions between the spirochetes and target cells. The present work demonstrates the direct binding of the Borrelia burgdorferi outer surface proteins OspA and OspB to CR3 and that this binding is independent of iC3b.

Temporal regulation of outer surface proteins of the Lyme-disease spirochaete Borrelia burgdorferi

2003 ◽  
Vol 31 (1) ◽  
pp. 108-112 ◽  
Author(s):  
T.G. Schwan
Keyword(s):  
Life Cycle ◽  
Lyme Disease ◽  
Borrelia Burgdorferi ◽  
Outer Surface ◽  
Surface Proteins ◽  
Temporal Regulation ◽  
Outer Surface Proteins ◽  
Vertebrate Hosts

In the 20 years since the first agent of Lyme disease was discovered, much interest has focused on the possible biological roles of a few outer surface proteins (Osps) in the alternating life cycle that includes ticks and vertebrate hosts. Two major proteins, OspA and OspC, are differentially regulated by the spirochaete Borrelia burgdorferi during the several days when ticks feed. The reciprocal decrease in OspA with the rapid up-regulation of OspC by the spirochaetes when ticks are feeding suggests that OspA aids in spirochaete attachment while OspC assists in the dissemination of spirochaetes from tick to vertebrate. Future experiments in ticks with mutant spirochaetes that lack these proteins should clarify the speculative functions currently given to these proteins.

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