Predicting Phospholipidosis: A Fluorescence Noncell Based in Vitro Assay for the Determination of Drug–Phospholipid Complex Formation in Early Drug Discovery

2011 ◽  
Vol 83 (18) ◽  
pp. 6980-6987 ◽  
Author(s):  
Liping Zhou ◽  
Gina Geraci ◽  
Sloan Hess ◽  
Linhong Yang ◽  
Jianling Wang ◽  
...  
Keyword(s):  
Drug Discovery ◽  
Complex Formation ◽  
In Vitro Assay ◽  
Phospholipid Complex ◽  
Vitro Assay ◽  
Early Drug

Applicability of the OECD 455 in-vitro assay for determination of hERa agonistic activity of isoflavonoids

2020 ◽  
Vol 386 ◽  
pp. 114831 ◽  
Author(s):  
Darja Gramec Skledar ◽  
Václav Tvrdý ◽  
Maša Kenda ◽  
Anamarija Zega ◽  
Milan Pour ◽  
...  
Keyword(s):  
In Vitro Assay ◽  
Vitro Assay ◽  
Agonistic Activity

scholarly journals Interleukin-6 Promotes Anti-OspA Borreliacidal Antibody Production In Vitro

2006 ◽  
Vol 13 (1) ◽  
pp. 19-25 ◽  
Author(s):  
Erik L. Munson ◽  
Dean T. Nardelli ◽  
K. H. Kevin Luk ◽  
Monica C. Remington ◽  
Steven M. Callister ◽  
...  
Keyword(s):  
Lymph Node ◽  
B Lymphocytes ◽  
Antibody Production ◽  
Interleukin 6 ◽  
In Vitro Assay ◽  
Vitro Assay ◽  
Lymph Node Cells ◽  
In Vitro Treatment

ABSTRACT Determination of the immunological mediators responsible for promoting the production of borreliacidal antibody may facilitate the development of an improved borreliosis vaccine for human and veterinary use. Previously, we developed an in vitro assay to determine if borreliacidal antibody production could be augmented by treatment with different cytokines. In this study, in vitro treatment of lymph node cells producing borreliacidal antibody with recombinant interleukin-6 (rIL-6) resulted in a fourfold enhancement of anti-OspA borreliacidal antibody. Moreover, rIL-6 enhanced Western immunoblot titers and increased the number of B lymphocytes. In contrast, treatment of anti-OspA borreliacidal antibody-producing cells with anti-IL-6 resulted in a fourfold reduction in borreliacidal activity. Treatment with anti-IL-6 also inhibited enhanced borreliacidal antibody production induced by anti-gamma interferon. These data suggest that IL-6 plays a significant role in the production of anti-OspA borreliacidal antibodies.

Spectrophotometric determination of de novo hemozoin/β-hematin formation in an in vitro assay

2004 ◽  
Vol 325 (1) ◽  
pp. 85-91 ◽  
Author(s):  
Abhai K Tripathi ◽  
Shabana I Khan ◽  
Larry A Walker ◽  
Babu L Tekwani
Keyword(s):  
Spectrophotometric Determination ◽  
De Novo ◽  
In Vitro Assay ◽  
Vitro Assay
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