Rapid Automatic Scanning Device for Paper Electrophoresis and Chromatography

1956 ◽  
Vol 28 (4) ◽  
pp. 558-558 ◽  
Author(s):  
D. Z. Silver ◽  
Ralph. Bookman
2018 ◽  
Vol 30 (5) ◽  
pp. 773-778 ◽  
Author(s):  
Femke E. M. Mulder ◽  
Sytske van der Velde ◽  
Fraukje Pol ◽  
Marjolein Bos ◽  
Jules Schagen van Leeuwen ◽  
...  

1971 ◽  
Vol 96 (1) ◽  
pp. 107-123 ◽  
Author(s):  
E.R. Flynn ◽  
T. Gardiner ◽  
M.J. Seamons ◽  
S.D. Orbesen

1951 ◽  
Vol 2 (2) ◽  
pp. 301-303 ◽  
Author(s):  
T CASPERSSON ◽  
F JACOBSSON ◽  
G LOMAKKA

1956 ◽  
Vol 27 (6) ◽  
pp. 370-374 ◽  
Author(s):  
Lennart W. Holm

1972 ◽  
Vol 71 (1) ◽  
pp. 24-36 ◽  
Author(s):  
Ariel Gordin ◽  
Pirkko Saarinen

ABSTRACT An account is given of a methodological study of the double-antibody radioimmunoassay of human TSH, using highly purified labelled human TSH as tracer. It was shown that conventional paper electrophoresis was not adequate for studying the purity of labelled human TSH. When polyvinylchloride (Pevikon®) electrophoresis was used, four subfractions could still be separated, even though, on paper electrophoresis, the material seemed to be homogeneous. Only two of the four Pevikon fractions were immunoreactive. Purification of labelled human TSH by Pevikon electrophoresis also improved the sensitivity of the assay. Specific activities of about 100 mCi/mg gave the highest initial binding capacity, produced least damage to the labelled hormone and showed the best stability of the tracer without influencing the sensitivity of the method. In different storage conditions, labelled human TSH was found to be most stable at −20°C and diluted 1/100. Only in pregnancy did the addition of HCG seem necessary. The mean TSH value in healthy subjects was 3.6 ± 1.4 μU/ml (mean±sd) with a range from 1.6 μU/ml to 8.8 μU/ml.


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