Single-Molecule Fluorescence Imaging of Peptide Binding to Supported Lipid Bilayers

2009 ◽  
Vol 81 (13) ◽  
pp. 5130-5138 ◽  
Author(s):  
Christopher B. Fox ◽  
Joshua R. Wayment ◽  
Grant A. Myers ◽  
Scott K. Endicott ◽  
Joel M. Harris
Keyword(s):  
Fluorescence Imaging ◽  
Lipid Bilayers ◽  
Single Molecule ◽  
Peptide Binding ◽  
Supported Lipid Bilayers ◽  
Single Molecule Fluorescence

scholarly journals Single molecule fluorescence reveals dimerization of myristoylated Src N-terminal region on supported lipid bilayers

2016 ◽  
Vol 1 (4) ◽  
pp. 642-647 ◽  
Author(s):  
Anabel-Lise Le Roux ◽  
Bruno Castro ◽  
Erik T. Garbacik ◽  
Maria F. Garcia Parajo ◽  
Miquel Pons
Keyword(s):  
Lipid Bilayers ◽  
Single Molecule ◽  
Terminal Region ◽  
Supported Lipid Bilayers ◽  
Single Molecule Fluorescence

Cover Picture: Single molecule fluorescence reveals dimerization of myristoylated Src N-terminal region on supported lipid bilayers (ChemistrySelect 4/2016)

2016 ◽  
Vol 1 (4) ◽  
pp. 641-641
Author(s):  
Anabel-Lise Le Roux ◽  
Bruno Castro ◽  
Erik T. Garbacik ◽  
Maria F. Garcia Parajo ◽  
Miquel Pons
Keyword(s):  
Lipid Bilayers ◽  
Single Molecule ◽  
Terminal Region ◽  
Supported Lipid Bilayers ◽  
Single Molecule Fluorescence

scholarly journals Single Molecule Fluorescence Imaging and Blinking

2006 ◽  
Vol 46 (3) ◽  
pp. 164-168
Author(s):  
Hiroaki YOKOTA ◽  
Tetsuichi WAZAWA ◽  
Yoshiharu ISHII
Keyword(s):  
Fluorescence Imaging ◽  
Single Molecule ◽  
Single Molecule Fluorescence

scholarly journals Low-Light Photodetectors for Fluorescence Microscopy

2021 ◽  
Vol 11 (6) ◽  
pp. 2773
Author(s):  
Hiroaki Yokota ◽  
Atsuhito Fukasawa ◽  
Minako Hirano ◽  
Toru Ide
Keyword(s):  
Fluorescence Microscopy ◽  
Fluorescence Imaging ◽  
Single Molecule ◽  
Science Studies ◽  
Single Photon ◽  
Laser Scanning Microscopy ◽  
Oxide Semiconductor ◽  
Wide Field ◽  
Single Molecule Fluorescence ◽  
Low Light

Over the years, fluorescence microscopy has evolved and has become a necessary element of life science studies. Microscopy has elucidated biological processes in live cells and organisms, and also enabled tracking of biomolecules in real time. Development of highly sensitive photodetectors and light sources, in addition to the evolution of various illumination methods and fluorophores, has helped microscopy acquire single-molecule fluorescence sensitivity, enabling single-molecule fluorescence imaging and detection. Low-light photodetectors used in microscopy are classified into two categories: point photodetectors and wide-field photodetectors. Although point photodetectors, notably photomultiplier tubes (PMTs), have been commonly used in laser scanning microscopy (LSM) with a confocal illumination setup, wide-field photodetectors, such as electron-multiplying charge-coupled devices (EMCCDs) and scientific complementary metal-oxide-semiconductor (sCMOS) cameras have been used in fluorescence imaging. This review focuses on the former low-light point photodetectors and presents their fluorescence microscopy applications and recent progress. These photodetectors include conventional PMTs, single photon avalanche diodes (SPADs), hybrid photodetectors (HPDs), in addition to newly emerging photodetectors, such as silicon photomultipliers (SiPMs) (also known as multi-pixel photon counters (MPPCs)) and superconducting nanowire single photon detectors (SSPDs). In particular, this review shows distinctive features of HPD and application of HPD to wide-field single-molecule fluorescence detection.

scholarly journals Correlated diffusion in lipid bilayers

2021 ◽  
Vol 118 (48) ◽  
pp. e2113202118
Author(s):  
Rafael L. Schoch ◽  
Frank L. H. Brown ◽  
Gilad Haran
Keyword(s):  
Lipid Bilayers ◽  
Single Molecule ◽  
Lipid Membranes ◽  
Supported Lipid Bilayers ◽  
Single Molecule Tracking ◽  
Black Lipid Membranes ◽  
Molecular Tracking ◽  
Physical Materials ◽  
Multiple Molecules ◽  
Major Target

Lipid membranes are complex quasi–two-dimensional fluids, whose importance in biology and unique physical/materials properties have made them a major target for biophysical research. Recent single-molecule tracking experiments in membranes have caused some controversy, calling the venerable Saffman–Delbrück model into question and suggesting that, perhaps, current understanding of membrane hydrodynamics is imperfect. However, single-molecule tracking is not well suited to resolving the details of hydrodynamic flows; observations involving correlations between multiple molecules are superior for this purpose. Here dual-color molecular tracking with submillisecond time resolution and submicron spatial resolution is employed to reveal correlations in the Brownian motion of pairs of fluorescently labeled lipids in membranes. These correlations extend hundreds of nanometers in freely floating bilayers (black lipid membranes) but are severely suppressed in supported lipid bilayers. The measurements are consistent with hydrodynamic predictions based on an extended Saffman–Delbrück theory that explicitly accounts for the two-leaflet bilayer structure of lipid membranes.

scholarly journals HDL particles incorporate into lipid bilayers – a combined AFM and single molecule fluorescence microscopy study

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Birgit Plochberger ◽  
Clemens Röhrl ◽  
Johannes Preiner ◽  
Christian Rankl ◽  
Mario Brameshuber ◽  
...  
Keyword(s):  
Fluorescence Microscopy ◽  
Lipid Bilayers ◽  
Single Molecule ◽  
Microscopy Study ◽  
Single Molecule Fluorescence ◽  
Single Molecule Fluorescence Microscopy
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