Free Glycerol Sensing in Biodiesel: Automatic System Based on Sequential Enzymatic Reactions Using a Fit-for-Purpose Chemiluminescence Detection Chamber

2019 ◽  
Vol 33 (10) ◽  
pp. 9925-9933
Author(s):  
Nicolás A. Nario ◽  
Matías Insausti ◽  
Claudia E. Domini ◽  
Marcos Grünhut
Keyword(s):  
Automatic System ◽  
Enzymatic Reactions ◽  
Free Glycerol ◽  
Chemiluminescence Detection ◽  
Detection Chamber ◽  
Fit For Purpose

Improving Body Satisfaction

2015 ◽  
Vol 20 (1) ◽  
pp. 62-71 ◽  
Author(s):  
Carolien Martijn ◽  
Jessica M. Alleva ◽  
Anita Jansen
Keyword(s):  
Eating Disorders ◽  
Body Dissatisfaction ◽  
Automatic System ◽  
Body Satisfaction ◽  
Western Society ◽  
Related Information ◽  
Current Article ◽  
Women And Girls

Feelings of body dissatisfaction are common in Western society, especially in women and girls. More than innocent discontent, body dissatisfaction can have serious consequences such as depression and eating disorders. The current article discusses the nature of body dissatisfaction, how it develops and how it is currently being treated. We also discuss novel strategies to increase body satisfaction that work on the automatic system (e.g., by retraining attentional and conditioning processes), since recent research suggests that appearance-related information is processed automatically. We suggest that extant methods should be combined with these novel strategies, in order to optimally improve body dissatisfaction and to prevent its detrimental consequences.

AUTOMATIC SYSTEM FOR MICROMECHANICAL PROPERTIES ANALYSIS

1981 ◽  
Vol 42 (C5) ◽  
pp. C5-1129-C5-1134
Author(s):  
S. Etienne ◽  
J. Y. Cavaille ◽  
J. Perez ◽  
M. Salvia
Keyword(s):  
Automatic System ◽  
Micromechanical Properties

Fit for Purpose: Using a Distance Education Approach to Support Underperforming Schools in South Africa

2018 ◽  
Vol 41 (1) ◽  
Author(s):  
Veronica Irene McKay
Keyword(s):  
Distance Education ◽  
Curriculum Reform ◽  
Mixed Methods Research ◽  
Research Approach ◽  
Educational Design ◽  
Learner Performance ◽  
Poor Learner ◽  
Fit For Purpose ◽  
Additional Support ◽  
Content Support

This article explores the South African government’s national school workbook intervention aimed at addressing poor learner performance in the context of teacher under-preparedness and curriculum reform. It shows how the workbooks use a distance education approach to provide pedagogical and content support for teachers, albeit in the context of classroom teaching, to compensate for teachers’ pedagogical challenges. This article uses a mixed methods research approach to explore how teachers, learners and parents used the workbooks and shows that while the distance educational design scaffolded teaching, additional support is necessary to enable the intervention to be more impactful. 

Development of 96 Multiple Injection-GC-MS Technique and Its Application in Protein Engineering of Natural and Non-Natural Enzymatic Reactions

2019 ◽  
Author(s):  
Anja Knorrscheidt ◽  
Pascal Püllmann ◽  
Eugen Schell ◽  
Dominik Homann ◽  
Erik Freier ◽  
...  
Keyword(s):  
Microtiter Plate ◽  
Axial Ligand ◽  
Cell System ◽  
Enzymatic Reactions ◽  
The Novel ◽  
Internal Standards ◽  
E Coli ◽  
Enzyme Screening ◽  
Plate Analysis ◽  
Microtiter Plate Analysis

Directed evolution requires the screening of enzyme libraries in biological matrices. Available assays are mostly substrate or enzyme specific. Chromatographic techniques like LC and GC overcome this limitation, but require long analysis times. The herein developed multiple injections in a single experimental run (MISER) using GC coupled to MS allows the injection of samples every 33 s resulting in 96-well microtiter plate analysis within 50 min. This technique is implementable in any GC-MS system with autosampling. Since the GC-MS is far less prone to ion suppression than LCMS, no chromatographic separation is required. This allows the utilisation of an internal standards and the detection of main and side-product. To prove the feasibility of the system in enzyme screening, two libraries were assessed: i) YfeX library in an E. coli whole cell system for the carbene-transfer reaction on indole revealing the novel axial ligand tryptophan, ii) a library of 616 chimeras of fungal unspecific peroxygenase (UPO) in S. cerevisiae supernatant for hydroxylation of tetralin resulting in novel constructs. The data quality and representation are automatically assessed by a new R-script.

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