Combinatorial Modulation of Linalool Synthase and Farnesyl Diphosphate Synthase for Linalool Overproduction in Saccharomyces cerevisiae

2021 ◽  
Vol 69 (3) ◽  
pp. 1003-1010
Author(s):  
Pingping Zhou ◽  
Yi Du ◽  
Xin Fang ◽  
Nannan Xu ◽  
Chunlei Yue ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Farnesyl Diphosphate ◽  
Farnesyl Diphosphate Synthase ◽  
Linalool Synthase

scholarly journals Increasing the intracellular isoprenoid pool in Saccharomyces cerevisiae by structural fine-tuning of a bifunctional farnesyl diphosphate synthase

2017 ◽  
Vol 17 (4) ◽  
Author(s):  
Sebastián Rubat ◽  
Ignacio Varas ◽  
Romina Sepúlveda ◽  
Daniel Almonacid ◽  
Fernando González-Nilo ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Farnesyl Diphosphate ◽  
Fine Tuning ◽  
Farnesyl Diphosphate Synthase

scholarly journals Functional relationships between the Saccharomyces cerevisiae cis-prenyltransferases required for dolichol biosynthesis.

2005 ◽  
Vol 52 (1) ◽  
pp. 221-232 ◽  
Author(s):  
Kariona Grabińska ◽  
Grazyna Sosińska ◽  
Jacek Orłowski ◽  
Ewa Swiezewska ◽  
Thierry Berges ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Enzymatic Activity ◽  
Farnesyl Diphosphate ◽  
Farnesyl Diphosphate Synthase ◽  
Gene Encoding ◽  
Yeast Saccharomyces Cerevisiae ◽  
Functional Relationships ◽  
Dolichyl Phosphate ◽  
Preferential Synthesis

In the yeast Saccharomyces cerevisiae the RER2 and SRT1 genes encode Rer2 and Srt1 proteins with cis-prenyltransferase (cis-PT-ase) activity. Both cis-PT-ases utilize farnesyl diphosphate (FPP) as a starter for polyprenyl diphosphate (dolichol backbone) formation. The products of the Rer2 and Srt1 proteins consist of 14-17 and 18-23 isoprene units, respectively. In this work we demonstrate that deletion or overexpression of SRT1 up-regulates the activity of Rer2p and dolichol content. However, upon overexpression of SRT1, preferential synthesis of longer-chain dolichols and a decrease in the amount of the shorter species are observed. Furthermore, overexpression of the ERG20 gene (encoding farnesyl diphosphate synthase, Erg20p) induces transcription of SRT1 mRNA and increases the levels of mRNA for RER2 and DPM1 (dolichyl phosphate mannose synthase, Dpm1p). Subsequently the enzymatic activity of Rer2p and dolichol content are also increased. However, the amount of Dpm1p or its enzymatic activity remain unchanged.

scholarly journals Inhibitors of Leishmania Major Farnesyl Diphosphate Synthase: Crystallographic and Calorimetric Studies

2011 ◽  
Vol 100 (3) ◽  
pp. 218a-219a
Author(s):  
Srinivas Aripirala ◽  
Sandra Gabelli ◽  
Eric Oldfield ◽  
Dolores Gonzalez Pacanowska ◽  
Mario Amzel
Keyword(s):  
Leishmania Major ◽  
Farnesyl Diphosphate ◽  
Farnesyl Diphosphate Synthase ◽  
Calorimetric Studies
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