scholarly journals Encoding Multiple Virtual Signals in DNA Barcodes with Single-Molecule FRET

Nano Letters ◽  
2021 ◽  
Vol 21 (4) ◽  
pp. 1694-1701 ◽  
Author(s):  
Sung Hyun Kim ◽  
Hyunwoo Kim ◽  
Hawoong Jeong ◽  
Tae-Young Yoon
Keyword(s):  
Single Molecule ◽  
Dna Barcodes ◽  
Single Molecule Fret

scholarly journals Encoding multiple virtual signals in DNA barcodes with single-molecule FRET

2020 ◽  
Author(s):  
Sung Hyun Kim ◽  
Hyunwoo Kim ◽  
Hawoong Jeong ◽  
Tae-Young Yoon
Keyword(s):  
Single Molecule ◽  
Dna Barcode ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Emission Spectra ◽  
Biological Molecules ◽  
Dna Barcodes ◽  
Signal Space ◽  
Single Molecule Fret

ABSTRACTDNA barcoding provides a way to label a huge number of different biological molecules using the extreme programmability in DNA sequence synthesis. Fluorescence imaging is an easy-to-access method to detect individual DNA barcodes, which can be scaled up to a massively high-throughput format. Large overlaps between emission spectra of fluorescence dyes, however, severely limit the numbers of DNA barcodes–and thus its signal space–that can be detected in a simultaneous manner. We here demonstrate the use of single-molecule fluorescence resonance energy transfer (FRET) to encode virtual signals in DNA barcodes using conventional two-color fluorescence microscopy. By optimizing imaging and biochemistry conditions for weak hybridization events for DNA barcodes, we markedly enhanced accuracy in our determination of the efficiency by which single-molecule FRET occurred. This allowed us to unambiguously differentiate six DNA barcodes exhibiting different FRET values without involving probe sequence exchanges. Our method can be directly incorporated with previous DNA barcode techniques, and may thus be widely adopted to expand the signal space of the DNA barcode techniques.

Single-Molecule FRET-Based Dynamic DNA Sensor

ACS Sensors ◽  
2021 ◽  
Author(s):  
Anoja Megalathan ◽  
Kalani M. Wijesinghe ◽  
Soma Dhakal
Keyword(s):  
Single Molecule ◽  
Dna Sensor ◽  
Single Molecule Fret

scholarly journals Single-molecule FRET dynamics of molecular motors in an ABEL trap

Methods ◽  
2021 ◽  
Author(s):  
Maria Dienerowitz ◽  
Jamieson A.L. Howard ◽  
Steven D. Quinn ◽  
Frank Dienerowitz ◽  
Mark C. Leake
Keyword(s):  
Single Molecule ◽  
Molecular Motors ◽  
Single Molecule Fret

scholarly journals High-Resolution Single-Molecule FRET via DNA eXchange (FRET X)

Nano Letters ◽  
2021 ◽  
Vol 21 (7) ◽  
pp. 3295-3301 ◽  
Author(s):  
Mike Filius ◽  
Sung Hyun Kim ◽  
Ivo Severins ◽  
Chirlmin Joo
Keyword(s):  
High Resolution ◽  
Single Molecule ◽  
Single Molecule Fret ◽  
Resolution Single

scholarly journals Role of the ribosomal protein L27 revealed by single-molecule FRET study

2012 ◽  
Vol 21 (11) ◽  
pp. 1696-1704 ◽  
Author(s):  
Yuhong Wang ◽  
Ming Xiao
Keyword(s):  
Ribosomal Protein ◽  
Single Molecule ◽  
Single Molecule Fret

scholarly journals Mapping the Structure of Tau Using Single Molecule FRET

2014 ◽  
Vol 106 (2) ◽  
pp. 270a
Author(s):  
Xiaohan Li ◽  
Elizabeth Rhoades
Keyword(s):  
Single Molecule ◽  
Single Molecule Fret
Sign in / Sign up

Export Citation Format

Share Document