scholarly journals High-Resolution Single-Molecule FRET via DNA eXchange (FRET X)

Nano Letters ◽  
2021 ◽  
Vol 21 (7) ◽  
pp. 3295-3301 ◽  
Author(s):  
Mike Filius ◽  
Sung Hyun Kim ◽  
Ivo Severins ◽  
Chirlmin Joo
Keyword(s):  
High Resolution ◽  
Single Molecule ◽  
Single Molecule Fret ◽  
Resolution Single

Global Structure of Forked DNA in Solution Revealed by High-Resolution Single-Molecule FRET

2011 ◽  
Vol 133 (5) ◽  
pp. 1188-1191 ◽  
Author(s):  
Tara Sabir ◽  
Gunnar F. Schröder ◽  
Anita Toulmin ◽  
Peter McGlynn ◽  
Steven W. Magennis
Keyword(s):  
High Resolution ◽  
Single Molecule ◽  
Global Structure ◽  
Single Molecule Fret ◽  
Resolution Single

scholarly journals High-Resolution Single-Molecule FRET via DNA eXchange (FRET X)

2020 ◽  
Author(s):  
Mike Filius ◽  
Sung Hyun Kim ◽  
Ivo Severins ◽  
Chirlmin Joo
Keyword(s):  
Structural Analysis ◽  
High Resolution ◽  
Single Molecule ◽  
Single Object ◽  
Single Molecule Fret ◽  
Dna Strands ◽  
Versatile Tool ◽  
Fret Efficiency ◽  
Fret Pair

ABSTRACTSingle-molecule FRET is a versatile tool to study nucleic acids and proteins at the nanometer scale. However, currently, only a couple of FRET pairs can be reliably measured on a single object. The limited number of available FRET pair fluorophores and complicated data analysis makes it challenging to apply single-molecule FRET for structural analysis of biomolecules. Currently, only a couple of FRET pairs can be reliably measured on a single object. Here we present an approach that allows for the determination of multiple distances between FRET pairs in a single object. We use programmable, transient binding between short DNA strands to resolve the FRET efficiency of multiple fluorophore pairs. By allowing only a single FRET pair to be formed at a time, we can determine the FRET efficiency and pair distance with sub-nanometer resolution. We determine the distance between other pairs by sequentially exchanging DNA strands. We name this multiplexing approach FRET X for FRET via DNA eXchange. We envision that our FRET X technology will be a tool for the high-resolution structural analysis of biomolecules and other nano-structures.

scholarly journals Rigid DNA Beams for High-Resolution Single-Molecule Mechanics

2013 ◽  
Vol 125 (30) ◽  
pp. 7920-7925 ◽  
Author(s):  
Emanuel Pfitzner ◽  
Christian Wachauf ◽  
Fabian Kilchherr ◽  
Benjamin Pelz ◽  
William M. Shih ◽  
...  
Keyword(s):  
High Resolution ◽  
Single Molecule ◽  
Resolution Single

scholarly journals High-Resolution Single-Molecule Fluorescence Imaging of Zeolite Aggregates within Real-Life Fluid Catalytic Cracking Particles

2014 ◽  
Vol 54 (6) ◽  
pp. 1836-1840 ◽  
Author(s):  
Zoran Ristanović ◽  
Marleen M. Kerssens ◽  
Alexey V. Kubarev ◽  
Frank C. Hendriks ◽  
Peter Dedecker ◽  
...  
Keyword(s):  
High Resolution ◽  
Fluorescence Imaging ◽  
Single Molecule ◽  
Catalytic Cracking ◽  
Real Life ◽  
Fluid Catalytic Cracking ◽  
Single Molecule Fluorescence ◽  
Resolution Single

scholarly journals Transcription-Factor-Mediated DNA Looping Probed by High-Resolution, Single-Molecule Imaging in Live E. coli Cells

PLoS Biology ◽  
2013 ◽  
Vol 11 (6) ◽  
pp. e1001591 ◽  
Author(s):  
Zach Hensel ◽  
Xiaoli Weng ◽  
Arvin Cesar Lagda ◽  
Jie Xiao
Keyword(s):  
Transcription Factor ◽  
High Resolution ◽  
Single Molecule ◽  
Dna Looping ◽  
Single Molecule Imaging ◽  
E Coli ◽  
Resolution Single

scholarly journals High Resolution Single Molecule Analysis using Nanopore Recording on Microelectrode Cavity Arrays

2011 ◽  
Vol 100 (3) ◽  
pp. 608a
Author(s):  
Gerhard Baaken ◽  
Srujan K. Dondapati ◽  
Norbert Ankri ◽  
Jürgen Rühe ◽  
Jan C. Behrends
Keyword(s):  
High Resolution ◽  
Single Molecule ◽  
Single Molecule Analysis ◽  
Resolution Single

scholarly journals (Un)Confined Diffusion of CD59 in the Plasma Membrane Determined by High-Resolution Single Molecule Microscopy

2007 ◽  
Vol 92 (10) ◽  
pp. 3719-3728 ◽  
Author(s):  
Stefan Wieser ◽  
Manuel Moertelmaier ◽  
Elke Fuertbauer ◽  
Hannes Stockinger ◽  
Gerhard J. Schütz
Keyword(s):  
Plasma Membrane ◽  
High Resolution ◽  
Single Molecule ◽  
Single Molecule Microscopy ◽  
Resolution Single

scholarly journals High-Resolution Single Molecule Rotation Tracking of RecBCD using DNA Origami Rotors

2017 ◽  
Vol 112 (3) ◽  
pp. 170a
Author(s):  
Benjamin D. Altheimer ◽  
Pallav Kosuri ◽  
Mingjie Dai ◽  
Peng Yin ◽  
Xiaowei Zhuang
Keyword(s):  
High Resolution ◽  
Single Molecule ◽  
Dna Origami ◽  
Resolution Single
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