We report a facile and efficient way to fabricate sensitive substrates for surface-enhanced Raman scattering (SERS) via sputtering Au nanoparticles on the surface of porous anodic aluminum oxide (AAO). This substrate could reduce the detection limit of Rhodamine 6G (R6G) to 10-12 M. Moreover, it exhibits excellent reproducibility and the relative standard deviation value of SERS intensity at 616 cm-1 is about 12%. More importantly, it can be scaled up for high-throughput production with low cost and large scale.