scholarly journals Correction to Development of Whole-Porcine Monoclonal Antibodies with Potent Neutralization Activity against Classical Swine Fever Virus from Single B Cells

2020 ◽  
Vol 9 (4) ◽  
pp. 978-978
Author(s):  
Haisi Dong ◽  
Ang Su ◽  
Dongmei Lv ◽  
Lerong Ma ◽  
Jianwei Dong ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
B Cells ◽  
Classical Swine Fever Virus ◽  
Classical Swine Fever ◽  
Fever Virus ◽  
Neutralization Activity ◽  
Potent Neutralization

scholarly journals Development of whole-porcine monoclonal antibodies with potent neutralization activity against classical swine fever virus (CSFV) from single B cells

2018 ◽  
Author(s):  
Haisi Dong ◽  
Dongmei Lv ◽  
Ang Su ◽  
Lerong Ma ◽  
Jianwei Dong ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
B Cells ◽  
Virus Infection ◽  
Classical Swine Fever Virus ◽  
Classical Swine Fever ◽  
Fever Virus ◽  
Economic Losses ◽  
E2 Protein ◽  
Neutralizing Activity

AbstractClassical swine fever (CSF) is a highly contagious swine disease found worldwide that has caused devastating economic losses. However, there are few efficacious mAbs against the CSF virus (CSFV) that can be used for treatment because most mAbs against CSFV are derived from mouse hybridoma cells and these murine mAbs have disadvantages of inefficient effector functions elicitations and high immunogenicity in vivo. Accordingly, we characterized whole-porcine anti-CSFV neutralizing mAbs (NAbs) isolated directly from single B cells sorted from a CSFV-vaccinated pig using the fluoresceinated conserved linear neutralizing epitope of the CSFV E2 protein and fluorophore conjugated goat anti-pig IgG. Immunoglobulin (Ig) genes were isolated via nested PCR, and two porcine mAbs termed HK24 and HK44 were produced. We determined that these mAbs can bind to E2 protein and recognize sites within this major antigenic epitope. In addition, we found that mAbs HK24 and HK44 exhibit potent neutralizing activity against CSFV, and they can protect PK-15 cells from infections in vitro with potent IC50 values of 9.3 μg/ml and 0.62 μg/ml, respectively. Notably, we demonstrated that these two mAbs can be used as novel reagents for detecting virus infection. These data suggest that our results not only provide a method for efficiently obtaining mAbs against CSFV but also offer promising mAb candidates for development of antibody-based diagnostic and antiviral agents.ImportanceNeutralizing monoclonal antibodies (NAbs) can prevent and may slow the spread of virus infection. The discovery of NAbs that recognize classical swine fever virus (CSFV) necessitates new technologies because the NAbs produced by immunization and hybridoma technology could not be transferred to in vivo research. Multiple full-length human therapeutic antibodies have been produced via single-cell polymerase chain reactions but whole-porcine NAbs for CSFV have not been generated. In this study, two whole-porcine mAbs, named HK24 and HK44, were isolated from epitope-specific single B cells. We demonstrate that these two mAbs have potent neutralizing activity against CSFV and can protect cells against viral infection. Therefore, they may facilitate the development of vaccines or antiviral drugs that offer the advantages of stability and low immunogenicity.

The Role of B Cells in the Immune Response to Pestivirus (Classical Swine Fever Virus)

2006 ◽  
Vol 135 (1) ◽  
pp. 32-41 ◽  
Author(s):  
P.J. Sánchez-Cordón ◽  
J.L. Romero-Trevejo ◽  
M. Pedrera ◽  
A.I. Raya ◽  
J.C. Gómez-Villamandos
Keyword(s):  
Immune Response ◽  
B Cells ◽  
Classical Swine Fever Virus ◽  
Classical Swine Fever ◽  
Fever Virus

Strain-Specific Monoclonal Antibodies to the E2 Protein of Classical Swine Fever Virus, Paderborn Strain

Hybridoma ◽  
2012 ◽  
Vol 31 (5) ◽  
pp. 340-346 ◽  
Author(s):  
Mariko Moniwa ◽  
Lizhong Luo ◽  
Kevin Hills ◽  
Krista Nishi ◽  
Erin MacLeod ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Classical Swine Fever Virus ◽  
Classical Swine Fever ◽  
Fever Virus ◽  
E2 Protein

Antigenic domains analysis of classical swine fever virus E2 glycoprotein by mutagenesis and conformation-dependent monoclonal antibodies

2010 ◽  
Vol 149 (2) ◽  
pp. 183-189 ◽  
Author(s):  
Chia-Yi Chang ◽  
Chin-Cheng Huang ◽  
Yu-Ju Lin ◽  
Ming-Chung Deng ◽  
Hui-Chun Chen ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Classical Swine Fever Virus ◽  
Classical Swine Fever ◽  
Fever Virus ◽  
E2 Glycoprotein ◽  
Antigenic Domains

scholarly journals RNase of classical swine fever virus: biochemical characterization and inhibition by virus-neutralizing monoclonal antibodies.

1996 ◽  
Vol 70 (1) ◽  
pp. 352-358 ◽  
Author(s):  
J M Windisch ◽  
R Schneider ◽  
R Stark ◽  
E Weiland ◽  
G Meyers ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Classical Swine Fever Virus ◽  
Biochemical Characterization ◽  
Classical Swine Fever ◽  
Fever Virus
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