Tuning Two-Photon Photoluminescence of Gold Nanoparticle Aggregates with DNA and Its Application as Turn-on Photoluminescence Probe for DNA Sequence Detection

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P. C. Mathur

A reliable, fast, and low-cost biosensor for medical diagnostics using DNA sequence detection has been developed and tested for the detection of the bacterium “Bacillus anthracis.” In this sensor, Poly [9,9-di (6,6′- N,N′trimethylammonium) hexylfluorenyl-2, 7-diyl)-alt-co- (1,4-phenylene)] dibromide salt (PFP) has been taken as cationic conjugated polymer (CCP) and PNA attached with fluorescein dye (PNAC∗) as a probe. The basic principle of this sensor is that when aPNAC∗probe is hybridized with a single strand DNA (ssDNA) having complementary sequence, Forster resonance energy transfer (FRET) may take place from PFP to thePNAC∗/DNA complex. If the FRET is efficient, the photoluminescence from the PFP will be highly quenched and that fromPNAC∗will be enhanced. On the other hand, if the DNA sequence is noncomplementary to PNA, FRET will not occur.



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