Pseudomonas mevalonii 3-hydroxy-3-methylglutaryl-CoA lyase: Characterization of the isolated recombinant protein and investigation of the enzyme's cation requirements

Biochemistry ◽  
1992 ◽  
Vol 31 (45) ◽  
pp. 11224-11230 ◽  
Author(s):  
Chakravarthy Narasimhan ◽  
Henry M. Miziorko
Keyword(s):  
Recombinant Protein ◽  
Pseudomonas Mevalonii

scholarly journals Cloning, expression, and antigenic characterization of recombinant protein ofMycoplasma gallisepticum expressed inEscherichia coli

2015 ◽  
Vol 94 (4) ◽  
pp. 621-627 ◽  
Author(s):  
T.S. Rocha ◽  
C. Tramuta ◽  
S. Catania ◽  
A. Matucci ◽  
M.G. Giuffrida ◽  
...  
Keyword(s):  
Recombinant Protein ◽  
Inescherichia Coli ◽  
Antigenic Characterization

Expression and characterization of the 42 kDa chitinase of the biocontrol fungusMetarhizium anisopliaeinEscherichia coli

2003 ◽  
Vol 49 (11) ◽  
pp. 723-726 ◽  
Author(s):  
César Milton Baratto ◽  
Marcia Vanusa da Silva ◽  
Lucélia Santi ◽  
Luciane Passaglia ◽  
Irene Silveira Schrank ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Recombinant Protein ◽  
Metarhizium Anisopliae ◽  
Entomopathogenic Fungus ◽  
Expression Vector ◽  
Hydrolytic Enzymes ◽  
Active Protein ◽  
Chitinase Genes

Albeit Metarhizium anisopliae is the best-characterized entomopathogenic fungus, the role of some hydrolytic enzymes during host cuticle penetration has not yet been established. Three chitinase genes (chit1, chi2, chi3) from Metarhizium have already been isolated. To characterize the chitinase coded by the chit1 gene, we expressed the active protein (CHIT42) in Escherichia coli using a T7-based promoter expression vector. The recombinant protein, CHIT42, is active against glycol chitin and synthetic N-acetylglucosamine (GlcNAc) dimer and tetramer substrates. These activities suggest that the recombinant CHIT42 acts as an endochitinase.Key words: Metarhizium anisopliae, chitinases, chit genes, recombinant protein, enthomopathogenic fungi.

scholarly journals Cloning and expression of Saccharomyces cerevisiae copper-metallothionein gene in Escherichia coli and characterization of the recombinant protein

1993 ◽  
Vol 212 (2) ◽  
pp. 521-528 ◽  
Author(s):  
Zehra SAYERS ◽  
Patricia BROUILLON ◽  
Constantin E. VORGIAS ◽  
Hans F. NOLTING ◽  
Christoph HERMES ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Saccharomyces Cerevisiae ◽  
Recombinant Protein ◽  
Cloning And Expression ◽  
Metallothionein Gene

scholarly journals Characterization of recombinant protein ferritin from Pyropia yezoensis (rPyFer) and its biological activities

2020 ◽  
Vol 29 (11) ◽  
pp. 1501-1509
Author(s):  
Selvakumari Ulagesan ◽  
Jeong-Wook Choi ◽  
Taek-Jeong Nam ◽  
Youn-Hee Choi
Keyword(s):  
Recombinant Protein ◽  
Biological Activities ◽  
Pyropia Yezoensis
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