Odorant Binding by a Pheromone Binding Protein: Active Site Mapping by Photoaffinity Labeling

Biochemistry ◽  
1994 ◽  
Vol 33 (16) ◽  
pp. 4812-4819 ◽  
Author(s):  
Gehua Du ◽  
Chi-Shing Ng ◽  
Glenn D. Prestwich
Keyword(s):  
Active Site ◽  
Binding Protein ◽  
Photoaffinity Labeling ◽  
Pheromone Binding Protein ◽  
Site Mapping ◽  
Odorant Binding ◽  
Protein Active Site

scholarly journals Bacterial expression and photoaffinity labeling of a pheromone binding protein

1993 ◽  
Vol 2 (3) ◽  
pp. 420-428 ◽  
Author(s):  
Glenn D. Prestwich
Keyword(s):  
Binding Protein ◽  
Photoaffinity Labeling ◽  
Bacterial Expression ◽  
Pheromone Binding Protein

Pheromone-binding protein and general odorant-binding protein of Sesamia nonagrioides: sex- and diel-dependent expression

2006 ◽  
Vol 119 (2) ◽  
pp. 129-136 ◽  
Author(s):  
M.A. Konstantopoulou ◽  
H. Pratsinis ◽  
D. Kletsas ◽  
B.E. Mazomenos
Keyword(s):  
Binding Protein ◽  
Odorant Binding Protein ◽  
Sesamia Nonagrioides ◽  
Pheromone Binding Protein ◽  
Odorant Binding

Labeling of amide linkages in active site mapping: Carbonium ion and extended photoaffinity labeling approaches

1978 ◽  
Vol 100 (23) ◽  
pp. 7421-7423 ◽  
Author(s):  
Emil H. White ◽  
H. Mark Perks ◽  
David F. Roswell
Keyword(s):  
Active Site ◽  
Photoaffinity Labeling ◽  
Carbonium Ion ◽  
Site Mapping

Generation of Polyclonal Antibody for Bombyx mori Pheromone-Binding Protein 1 (BmPBP1)

2013 ◽  
Vol 796 ◽  
pp. 15-20
Author(s):  
Kai Zun Xu ◽  
Ming Hui Wang ◽  
Lie Ma ◽  
Guo Sheng Li ◽  
Chao Mei ◽  
...  
Keyword(s):  
Sex Pheromone ◽  
Bombyx Mori ◽  
Polyclonal Antibody ◽  
Binding Protein ◽  
Information Communication ◽  
Male And Female ◽  
Odorant Binding Proteins ◽  
Pheromone Binding Protein ◽  
Moth Antenna ◽  
Odorant Binding

Bombyx mori pheromone-Binding Protein 1 (BmPBP1) in male moth antennae is a class of Odorant-Binding Proteins (OBPs), it can bind with the specific sex pheromone from female moth, thus initiates the males behaviors like seeking and mating, etc. It has been found that sex pheromone-binding protein 1 is differentially expressed in the antenna of male and female silkworm moths, however, the molecular mechanism of different PBP1 expression and its role in the information transmission are unclear. In this study, we successfully generated the BmPBP1 polyclonal antibody and used it to detect BmPBP1 expression in the silkworm moth antenna. Thus this work is helpful for further studies on the function of BmPBP1 in the information communication between male and female moths.

scholarly journals The Streptomyces K15 DD-peptidase/penicillin-binding protein. Active site and sequence of the N-terminal region

1989 ◽  
Vol 260 (2) ◽  
pp. 601-604 ◽  
Author(s):  
M Leyh-Bouille ◽  
J Van Beeumen ◽  
S Renier-Pirlot ◽  
B Joris ◽  
M Nguyen-Distèche ◽  
...  
Keyword(s):  
Signal Peptide ◽  
Active Site ◽  
Binding Protein ◽  
Terminal Region ◽  
High Homology ◽  
Penicillin Binding Protein ◽  
Serine Residue ◽  
Protein Active Site

The N-terminal region of the Streptomyces K15 DD-peptidase/penicillin-binding protein shows high homology with that of other penicillin-interactive proteins or domains. The active-site serine residue of the conserved tetrad Ser-Xaa-Xaa-Lys occurs at position 35. There is no indication for the presence of a signal peptide or an N-terminal hydrophobic sequence, suggesting that the Streptomyces K15 enzyme is probably anchored to the membrane by a C-terminal peptide segment.

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