Lactate dehydrogenase catalyzed pyruvate adduct reaction: simulation general acid-base catalysis involving an enzyme and an external catalyst

Biochemistry ◽  
1984 ◽  
Vol 23 (16) ◽  
pp. 3626-3635 ◽  
Author(s):  
John W. Burgner ◽  
William J. Ray
Keyword(s):  
Lactate Dehydrogenase ◽  
Base Catalysis ◽  
Acid Base ◽  
General Acid

scholarly journals Chiral recognition of prochiral centres and general acid-base catalysis. Necessarily interrelated manifestations of active-site structure

1976 ◽  
Vol 153 (2) ◽  
pp. 491-493 ◽  
Author(s):  
J Südi
Keyword(s):  
Lactate Dehydrogenase ◽  
Active Site ◽  
Chiral Recognition ◽  
Conformational Transition ◽  
Base Catalysis ◽  
Acid Base ◽  
Site Structure ◽  
General Acid ◽  
Active Site Structure

On the basis of Ogston's [(1948) Nature (London) 162, 963] argument, the following conclusions are indicated by the stereochemistry of the reversible oxidation of glycollate by lactate dehydrogenase: (1) general acid-base catalysis is involved in the reaction; (2) the transformation of enzyme-bound (tetrahedral) substrate into enzyme-bound (trigonal) product involves a conformational transition of the enzyme-coenzyme complex.

Effect of Buffer General Acid−Base Catalysis on the Stereoselectivity of Ester and Thioester H/D Exchange in D2O

2011 ◽  
Vol 133 (13) ◽  
pp. 5124-5128 ◽  
Author(s):  
Jerry R. Mohrig ◽  
Nicholas J. Reiter ◽  
Randy Kirk ◽  
Michelle R. Zawadski ◽  
Nathan Lamarre-Vincent
Keyword(s):  
Base Catalysis ◽  
Acid Base ◽  
General Acid

Reverse Protonation Is the Key to General Acid−Base Catalysis in Enolase†,‡

Biochemistry ◽  
2003 ◽  
Vol 42 (27) ◽  
pp. 8298-8306 ◽  
Author(s):  
Paul A. Sims ◽  
Todd M. Larsen ◽  
Russell R. Poyner ◽  
W. Wallace Cleland ◽  
George H. Reed
Keyword(s):  
Base Catalysis ◽  
Acid Base ◽  
General Acid ◽  
Reverse Protonation
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