Chorismate mutase/prephenate dehydrogenase from Escherichia coli K12: purification, characterization, and identification of a reactive cysteine

Biochemistry ◽  
1984 ◽  
Vol 23 (25) ◽  
pp. 6240-6249 ◽  
Author(s):  
Graham S. Hudson ◽  
Vic Wong ◽  
Barrie E. Davidson
Keyword(s):  
Escherichia Coli ◽  
Chorismate Mutase ◽  
Escherichia Coli K12 ◽  
Prephenate Dehydrogenase

scholarly journals Feedback Inhibition of Chorismate Mutase/Prephenate Dehydrogenase (TyrA) of Escherichia coli: Generation and Characterization of Tyrosine-Insensitive Mutants

2005 ◽  
Vol 71 (11) ◽  
pp. 7224-7228 ◽  
Author(s):  
Tina Lütke-Eversloh ◽  
Gregory Stephanopoulos
Keyword(s):  
Escherichia Coli ◽  
Dna Sequences ◽  
Molecular Level ◽  
Feedback Inhibition ◽  
Feedback Regulation ◽  
The Other ◽  
Chorismate Mutase ◽  
Amino Acid Substitutions ◽  
Prephenate Dehydrogenase

ABSTRACT In order to get insights into the feedback regulation by tyrosine of the Escherichia coli chorismate mutase/prephenate dehydrogenase (CM/PDH), which is encoded by the tyrA gene, feedback-inhibition-resistant (fbr) mutants were generated by error-prone PCR. The tyrA fbr mutants were selected by virtue of their resistance toward m-fluoro-d,l-tyrosine, and seven representatives were characterized on the biochemical as well as on the molecular level. The PDH activities of the purified His6-tagged TyrA proteins exhibited up to 35% of the enzyme activity of TyrAWT, but tyrosine did not inhibit the mutant PDH activities. On the other hand, CM activities of the TyrAfbr mutants were similar to those of the TyrAWT protein. Analyses of the DNA sequences of the tyrA genes revealed that tyrA fbr contained amino acid substitutions either at Tyr263 or at residues 354 to 357, indicating that these two sites are involved in the feedback inhibition by tyrosine.

pH Dependency of the reactions catalyzed by chorismate mutase-prephenate dehydrogenase from Escherichia coli

Biochemistry ◽  
1991 ◽  
Vol 30 (31) ◽  
pp. 7777-7782 ◽  
Author(s):  
Joanne Turnbull ◽  
W. W. Cleland ◽  
John F. Morrison
Keyword(s):  
Escherichia Coli ◽  
Chorismate Mutase ◽  
Prephenate Dehydrogenase ◽  
Ph Dependency
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