Purification and characterization of an acetylcholine receptor from mammalian skeletal muscle

Biochemistry ◽  
1977 ◽  
Vol 16 (23) ◽  
pp. 5053-5060 ◽  
Author(s):  
J. Oliver Dolly ◽  
Eric A. Barnard
Keyword(s):  
Skeletal Muscle ◽  
Acetylcholine Receptor ◽  
Mammalian Skeletal Muscle ◽  
Purification And Characterization

scholarly journals A novel synapse-associated noncoding RNA.

1994 ◽  
Vol 14 (11) ◽  
pp. 7095-7104 ◽  
Author(s):  
M A Velleca ◽  
M C Wallace ◽  
J P Merlie
Keyword(s):  
Skeletal Muscle ◽  
Nucleotide Sequence ◽  
Postnatal Development ◽  
Acetylcholine Receptor ◽  
Noncoding Rna ◽  
Subtractive Hybridization ◽  
Open Reading Frames ◽  
Early Postnatal Development ◽  
Reading Frames

Synaptic nuclei of innervated muscle transcribe acetylcholine receptor (AChR) genes at a much higher level than extrasynaptic nuclei. To isolate candidate synaptic regulatory molecules responsible for the unique transcriptional potential of synaptic nuclei, we have taken a subtractive hybridization approach. Here, we report the cloning and characterization of a novel synapse-associated RNA, 7H4. 7H4 is expressed selectively in the endplate zone of skeletal muscle and is upregulated during early postnatal development and after denervation. Interestingly, the 7H4 gene has no introns, and yet two different-size RNAs with identical polyadenylated 3' ends are generated. Most intriguingly, the nucleotide sequence does not contain any significant open reading frames, suggesting that 7H4 may function as a noncoding RNA.

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