Butyl isocyanate, an active-site-specific reagent for yeast alcohol dehydrogenase

Carboxyl groups near the active site zinc contribute to catalysis in yeast alcohol dehydrogenase.

Journal of Biological Chemistry ◽

10.1016/s0021-9258(18)60737-9 ◽

1988 ◽

Vol 263 (11) ◽

pp. 5446-5454

Author(s):

A J Ganzhorn ◽

B V Plapp

Keyword(s):

Alcohol Dehydrogenase ◽

Active Site ◽

Carboxyl Groups ◽

Active site specific cadmium(II)-substituted horse liver alcohol dehydrogenase: crystal structures of the free enzyme, its binary complex with NADH, and the ternary complex with NADH and bound p-bromobenzyl alcohol

Biochemistry ◽

10.1021/bi00346a070 ◽

1985 ◽

Vol 24 (25) ◽

pp. 7503-7510 ◽

Cited By ~ 17

Author(s):

Gunter Schneider ◽

Eila Cedergren-Zeppezauer ◽

Stefan Knight ◽

Hans Eklund ◽

Michael Zeppezauer

Keyword(s):

Alcohol Dehydrogenase ◽

Crystal Structures ◽

Active Site ◽

Ternary Complex ◽

Free Enzyme ◽

Binary Complex ◽

Horse Liver Alcohol Dehydrogenase ◽

Site Specific ◽

Horse Liver

Probing the Active Site of Yeast Alcohol Dehydrogenase through Microscale Yeast-Mediated Reductions of Acetophenone and Acetylpyridines. A Collaborative and Research-Based Advanced Bioorganic Chemistry Laboratory Project

Journal of Chemical Education ◽

10.1021/ed077p363 ◽

2000 ◽

Vol 77 (3) ◽

pp. 363 ◽

Cited By ~ 1

Author(s):

Michael Carnahan ◽

Kari Cox ◽

Joey Espinosa ◽

Ray Feaster ◽

Robert Hirsch ◽

...

Keyword(s):

Bioorganic Chemistry ◽

Alcohol Dehydrogenase ◽

Active Site ◽

Chemistry Laboratory ◽

A hydrophobic region at the active site of yeast alcohol dehydrogenase

Biochemical and Biophysical Research Communications ◽

10.1016/0006-291x(64)90336-5 ◽

1964 ◽

Vol 16 (3) ◽

pp. 258-262 ◽

Cited By ~ 18

Author(s):

Bruce M. Anderson ◽

Constance D. Anderson

Keyword(s):

Alcohol Dehydrogenase ◽

Active Site ◽

Hydrophobic Region ◽

Active-site-specific zinc-depleted and reconstituted cobalt(II) human-liver alcohol dehydrogenase. Preparation, characterization and complexation with NADH and trans-4-(N,N-dimethylamino)-cinnamaldehyde

European Journal of Biochemistry ◽

10.1111/j.1432-1033.1988.tb13995.x ◽

1988 ◽

Vol 173 (2) ◽

pp. 275-280 ◽

Cited By ~ 5

Author(s):

Helga SCHNEIDER-BERNLOHR ◽

Grazyna FORMICKA-KOZLOWSKA ◽

Rolf BUHLER ◽

Jean-Pierre WARTBURG ◽

Michael ZEPPEZAUER

Keyword(s):

Alcohol Dehydrogenase ◽

Active Site ◽

Human Liver ◽

Site Specific ◽

Liver Alcohol Dehydrogenase

Interaction of an epoxide with yeast alcohol dehydrogenase. Evidence for binding and the modification of two active site cysteins by styrene oxide

Biochemistry ◽

10.1021/bi00683a002 ◽

1975 ◽

Vol 14 (12) ◽

pp. 2568-2574 ◽

Cited By ~ 12

Author(s):

Judith P. Klinman

Keyword(s):

Alcohol Dehydrogenase ◽

Active Site ◽

Styrene Oxide ◽

Active-Site-Specific Reconstituted Cobalt(II) Horse-Liver Alcohol Dehydrogenase. Changes of the Spectra of the Substrate trans-4-(N,N-Dimethylamino)-cinnamaldehyde and of the Catalytic Cobalt Ion upon Ternary Complex Formation with NADH and 1,4,5,6-Tetrahydronkotinamide-Adenine Dinucleotide

European Journal of Biochemistry ◽

10.1111/j.1432-1033.1979.tb02057.x ◽

1979 ◽

Vol 100 (1) ◽

pp. 267-270 ◽

Cited By ~ 28

Author(s):

Helmut DIETRICH ◽

Wolfgang MARET ◽

Leif WALLEN ◽

Michael ZEPPEZAUER

Keyword(s):

Complex Formation ◽

Alcohol Dehydrogenase ◽

Active Site ◽

Ternary Complex ◽

Horse Liver Alcohol Dehydrogenase ◽

Site Specific ◽

Cobalt Ion ◽

Ternary Complex Formation ◽

Horse Liver

Active site-specific reconstituted copper(II) horse liver alcohol dehydrogenase: A biological model for type 1 Cu2+ and its changes upon ligand binding and conformational transitions

Journal of Inorganic Biochemistry ◽

10.1016/s0162-0134(00)80205-6 ◽

1980 ◽

Vol 12 (3) ◽

pp. 241-252 ◽

Cited By ~ 43

Author(s):

Wolfgang Maret ◽

Helmut Dietrich ◽

Hans-Heinrich Ruf ◽

Michael Zeppezauer

Keyword(s):

Alcohol Dehydrogenase ◽

Ligand Binding ◽

Active Site ◽

Conformational Transitions ◽

Biological Model ◽

Horse Liver Alcohol Dehydrogenase ◽

Site Specific ◽

Horse Liver

Active-site sulfhydryl groups of yeast alcohol dehydrogenase

Biochemistry ◽

10.1021/bi00739a013 ◽

1973 ◽

Vol 12 (15) ◽

pp. 2856-2862 ◽

Cited By ~ 34

Author(s):

Jer-Shung Twu ◽

Christopher C. Q. Chin ◽

Finn Wold

Keyword(s):

Alcohol Dehydrogenase ◽

Active Site ◽

Sulfhydryl Groups ◽

Substrate specificity and stereoselectivity of horse liver alcohol dehydrogenase. Kinetic evaluation of binding and activation parameters controlling the catalytic cycles of unbranched, acyclic secondary alcohols and ketones as substrates of the native and active-site-specific Co(II)-substituted enzyme

European Journal of Biochemistry ◽

10.1111/j.1432-1033.1991.tb16322.x ◽

1991 ◽

Vol 201 (3) ◽

pp. 615-625 ◽

Cited By ~ 34

Author(s):

Hans W. ADOLPH ◽

Patrik MAURER ◽

Helga SCHNEIDER-BERNLOHR ◽

Christian SARTORIUS ◽

Michael ZEPPEZAUER

Keyword(s):

Alcohol Dehydrogenase ◽

Substrate Specificity ◽

Active Site ◽

Activation Parameters ◽

Secondary Alcohols ◽

Kinetic Evaluation ◽

Site Specific ◽

Horse Liver ◽

Catalytic Cycles