Disulfide Bond Isomerization in Prokaryotes†

Biochemistry ◽  
2003 ◽  
Vol 42 (5) ◽  
pp. 1179-1185 ◽  
Author(s):  
Annie Hiniker ◽  
James C. A. Bardwell
Keyword(s):  
Disulfide Bond ◽  
Disulfide Bond Isomerization

scholarly journals Furin Cleavage Potentiates the Membrane Fusion-Controlling Intersubunit Disulfide Bond Isomerization Activity of Leukemia Virus Env

2006 ◽  
Vol 80 (11) ◽  
pp. 5540-5551 ◽  
Author(s):  
Mathilda Sjöberg ◽  
Michael Wallin ◽  
Birgitta Lindqvist ◽  
Henrik Garoff
Keyword(s):  
Membrane Fusion ◽  
Disulfide Bond ◽  
Fusion Reaction ◽  
Leukemia Virus ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Furin Cleavage ◽  
Membrane Fusion Protein ◽  
Peptide Complexes ◽  
Disulfide Bond Isomerization

ABSTRACT The membrane fusion protein of murine leukemia virus is a trimer of a disulfide-linked peripheral-transmembrane (SU-TM) subunit complex. The intersubunit disulfide bond is in SU linked to a disulfide bond isomerization motif, CXXC, with which the virus controls its fusion reaction (M. Wallin, M. Ekström, and H. Garoff, EMBO J. 23:54-65, 2004). Upon receptor binding the isomerase rearranges the intersubunit disulfide bond into a disulfide bond isomer within the motif. This facilitates SU dissociation and fusion activation in the TM subunit. In the present study we have asked whether furin cleavage of the Env precursor potentiates the isomerase to be triggered. To this end we accumulated the late form of the precursor, gp90, in the cell by incubation in the presence of a furin-inhibiting peptide. The isomerization was done by NP-40 incubation or by a heat pulse under alkylation-free conditions. The cells were lysed in the presence of alkylator, and the precursor was immunoprecipitated, gel isolated, deglycosylated, and subjected to complete trypsin digestion. Disulfide-linked peptide complexes were separated by sodium dodecyl sulfate-tricine-polyacrylamide gel electrophoresis under nonreducing conditions. This assay revealed the size of the characteristic major disulfide-linked peptide complex that differentiates the two isomers of the disulfide bond between Cys336 (or Cys339) and Cys563, i.e., the bond corresponding to the intersubunit disulfide bond. The analyses showed that the isomerase was five- to eightfold more resistant to triggering in the precursor than in the mature, cleaved form. This suggests that the isomerase becomes potentiated for triggering by a structural change in Env that is induced by furin cleavage in the cell.

scholarly journals Disulfide Bond Isomerization and the Assembly of MHC Class I-Peptide Complexes

Immunity ◽  
2002 ◽  
Vol 16 (1) ◽  
pp. 87-98 ◽  
Author(s):  
Tobias P Dick ◽  
Naveen Bangia ◽  
David R Peaper ◽  
Peter Cresswell
Keyword(s):  
Mhc Class I ◽  
Disulfide Bond ◽  
Class I ◽  
Peptide Complexes ◽  
Disulfide Bond Isomerization

13C-NMR studies on disulfide bond isomerization in bovine pancreatic trypsin inhibitor (BPTI)

2016 ◽  
Vol 66 (1) ◽  
pp. 37-53 ◽  
Author(s):  
Mitsuhiro Takeda ◽  
Yohei Miyanoiri ◽  
Tsutomu Terauchi ◽  
Masatsune Kainosho
Keyword(s):  
Disulfide Bond ◽  
Trypsin Inhibitor ◽  
13C Nmr ◽  
Bovine Pancreatic Trypsin Inhibitor ◽  
Nmr Studies ◽  
Pancreatic Trypsin Inhibitor ◽  
Disulfide Bond Isomerization

Disulfide bond isomerization in BPTI and BPTI(G36S): An NMR study of correlated mobility in proteins

Biochemistry ◽  
1993 ◽  
Vol 32 (14) ◽  
pp. 3571-3582 ◽  
Author(s):  
Gottfried Otting ◽  
Edvards Liepinsh ◽  
Kurt Wuethrich
Keyword(s):  
Disulfide Bond ◽  
Nmr Study ◽  
Disulfide Bond Isomerization

scholarly journals Disulfide bond isomerization in prokaryotes

2008 ◽  
Vol 1783 (4) ◽  
pp. 530-534 ◽  
Author(s):  
Stefan Gleiter ◽  
James C.A. Bardwell
Keyword(s):  
Disulfide Bond ◽  
Disulfide Bond Isomerization

scholarly journals Catalysis of Protein Disulfide Bond Isomerization in a Homogeneous Substrate†

Biochemistry ◽  
2005 ◽  
Vol 44 (36) ◽  
pp. 12168-12178 ◽  
Author(s):  
Elizabeth A. Kersteen ◽  
Seth R. Barrows ◽  
Ronald T. Raines
Keyword(s):  
Disulfide Bond ◽  
Disulfide Bond Isomerization ◽  
Protein Disulfide
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