Contribution to Stability and Folding of a Buried Polar Residue at the CARM1 Methylation Site of the KIX Domain of CBP†

Biochemistry ◽  
2003 ◽  
Vol 42 (23) ◽  
pp. 7044-7049 ◽  
Author(s):  
Yu Wei ◽  
Jia-Cherng Horng ◽  
Andrew C. Vendel ◽  
Daniel P. Raleigh ◽  
Kevin J. Lumb
Keyword(s):  
Methylation Site ◽  
Polar Residue ◽  
Kix Domain

scholarly journals RecQL5 Promotes Genome Stabilization through Two Parallel Mechanisms—Interacting with RNA Polymerase II and Acting as a Helicase

2010 ◽  
Vol 30 (10) ◽  
pp. 2460-2472 ◽  
Author(s):  
M. Nurul Islam ◽  
David Fox ◽  
Rong Guo ◽  
Takemi Enomoto ◽  
Weidong Wang
Keyword(s):  
Rna Polymerase ◽  
Rna Polymerase Ii ◽  
Genome Stability ◽  
Transcriptional Regulators ◽  
Helicase Activity ◽  
Parallel Mechanisms ◽  
Recq Helicase ◽  
Pol Ii ◽  
Kix Domain ◽  
Genome Stabilization

ABSTRACT The RecQL5 helicase is essential for maintaining genome stability and reducing cancer risk. To elucidate its mechanism of action, we purified a RecQL5-associated complex and identified its major component as RNA polymerase II (Pol II). Bioinformatics and structural modeling-guided mutagenesis revealed two conserved regions in RecQL5 as KIX and SRI domains, already known in transcriptional regulators for Pol II. The RecQL5-KIX domain binds both initiation (Pol IIa) and elongation (Pol IIo) forms of the polymerase, whereas the RecQL5-SRI domain interacts only with the elongation form. Fully functional RecQL5 requires both helicase activity and associations with the initiation polymerase, because mutants lacking either activity are partially defective in the suppression of sister chromatid exchange and resistance to camptothecin-induced DNA damage, and mutants lacking both activities are completely defective. We propose that RecQL5 promotes genome stabilization through two parallel mechanisms: by participation in homologous recombination-dependent DNA repair as a RecQ helicase and by regulating the initiation of Pol II to reduce transcription-associated replication impairment and recombination.

scholarly journals Change in dimerization mode by removal of a single unsatisfied polar residue located at the interface

2000 ◽  
Vol 9 (9) ◽  
pp. 1852-1855 ◽  
Author(s):  
P. R. Pokkuluri ◽  
X. Cai ◽  
G. Johnson ◽  
F. J. Stevens ◽  
M. Schiffer
Keyword(s):  
Polar Residue

Mutational analysis of the KIX domain of CBP reveals residues critical for SREBP binding

FEBS Letters ◽  
2003 ◽  
Vol 554 (3) ◽  
pp. 403-409 ◽  
Author(s):  
Ya-Ping Liu ◽  
Ching-Wen Chang ◽  
Kung-Yao Chang
Keyword(s):  
Mutational Analysis ◽  
Kix Domain

scholarly journals Kix domain specific Immunoglobulin A can protect from adverse lung and cerebral pathology induced by Plasmodium berghei ANKA

2015 ◽  
Vol 464 (3) ◽  
pp. 943-948 ◽  
Author(s):  
Anil Kumar ◽  
Sapna Deore ◽  
Santosh Kumar ◽  
Tanuja Bankar ◽  
Anil Lotke ◽  
...  
Keyword(s):  
Plasmodium Berghei ◽  
Immunoglobulin A ◽  
Plasmodium Berghei Anka ◽  
Domain Specific ◽  
Specific Immunoglobulin ◽  
Cerebral Pathology ◽  
Kix Domain

scholarly journals LysargiNase mirrors trypsin for protein C-terminal and methylation-site identification

2014 ◽  
Vol 12 (1) ◽  
pp. 55-58 ◽  
Author(s):  
Pitter F Huesgen ◽  
Philipp F Lange ◽  
Lindsay D Rogers ◽  
Nestor Solis ◽  
Ulrich Eckhard ◽  
...  
Keyword(s):  
Protein C ◽  
Methylation Site ◽  
Site Identification

NMR Mapping of the HIV-1 Tat Interaction Surface of the KIX Domain of the Human Coactivator CBP†

Biochemistry ◽  
2004 ◽  
Vol 43 (4) ◽  
pp. 904-908 ◽  
Author(s):  
Andrew C. Vendel ◽  
Kevin J. Lumb
Keyword(s):  
Kix Domain ◽  
Interaction Surface ◽  
Hiv 1

scholarly journals Dual Labeling of the CBP/p300 KIX Domain for19F NMR Leads to Identification of a New Small-Molecule Binding Site

ChemBioChem ◽  
2018 ◽  
Vol 19 (9) ◽  
pp. 963-969 ◽  
Author(s):  
Clifford T. Gee ◽  
Keith E. Arntson ◽  
Edward J. Koleski ◽  
Rachel Lynn Staebell ◽  
William C. K. Pomerantz
Keyword(s):  
Binding Site ◽  
Small Molecule ◽  
Kix Domain
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