Site-Directed Mutagenesis of Putative Active Site Residues ofMunI Restriction Endonuclease:  Replacement of Catalytically Essential Carboxylate Residues Triggers DNA Binding Specificity†

Biochemistry ◽  
1997 ◽  
Vol 36 (37) ◽  
pp. 11086-11092 ◽  
Author(s):  
Arunas Lagunavicius ◽  
Virginijus Siksnys
Keyword(s):  
Dna Binding ◽  
Restriction Endonuclease ◽  
Active Site ◽  
Binding Specificity ◽  
Site Directed Mutagenesis ◽  
Directed Mutagenesis ◽  
Active Site Residues ◽  
Dna Binding Specificity ◽  
Putative Active Site

DNA Binding Specificity ofMunI Restriction Endonuclease Is Controlled by pH and Calcium Ions:  Involvement of Active Site Carboxylate Residues†

Biochemistry ◽  
1997 ◽  
Vol 36 (37) ◽  
pp. 11093-11099 ◽  
Author(s):  
Arunas Lagunavicius ◽  
Saulius Grazulis ◽  
Egle Balciunaite ◽  
Darius Vainius ◽  
Virginijus Siksnys
Keyword(s):  
Dna Binding ◽  
Restriction Endonuclease ◽  
Calcium Ions ◽  
Active Site ◽  
Binding Specificity ◽  
Dna Binding Specificity

Site-Directed Mutagenesis of Putative Active Site Residues of 5-Enolpyruvylshikimate-3-phosphate Synthase†

Biochemistry ◽  
1999 ◽  
Vol 38 (1) ◽  
pp. 296-302 ◽  
Author(s):  
Wendy A. Shuttleworth ◽  
Melissa E. Pohl ◽  
Gregory L. Helms ◽  
David L. Jakeman ◽  
Jeremy N. S. Evans
Keyword(s):  
Active Site ◽  
Site Directed Mutagenesis ◽  
Directed Mutagenesis ◽  
Active Site Residues ◽  
Putative Active Site ◽  
Phosphate Synthase

scholarly journals Site-directed mutagenesis of active site residues in a class I endochitinase from chestnut seeds

Glycobiology ◽  
1998 ◽  
Vol 8 (10) ◽  
pp. 1021-1028 ◽  
Author(s):  
G. Garcia-Casado ◽  
C. Collada ◽  
I. Allona ◽  
R. Casado ◽  
L. F. Pacios ◽  
...  
Keyword(s):  
Active Site ◽  
Site Directed Mutagenesis ◽  
Class I ◽  
Directed Mutagenesis ◽  
Active Site Residues

Magnesium(2+) confers DNA binding specificity to the EcoRV restriction endonuclease

Biochemistry ◽  
1992 ◽  
Vol 31 (15) ◽  
pp. 3727-3732 ◽  
Author(s):  
Vera Thielking ◽  
Ursel Selent ◽  
Eleonore Koehler ◽  
Axel Landgraf ◽  
Heiner Wolfes ◽  
...  
Keyword(s):  
Dna Binding ◽  
Restriction Endonuclease ◽  
Binding Specificity ◽  
Dna Binding Specificity
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