Characterization of the Nucleotide Binding Properties of SV40 T Antigen Using Fluorescent 3‘(2‘)-O-(2,4,6-Trinitrophenyl)adenine Nucleotide Analogues†

Biochemistry ◽  
1998 ◽  
Vol 37 (44) ◽  
pp. 15336-15344 ◽  
Author(s):  
Shu-Gui Huang ◽  
Klaus Weisshart ◽  
Ellen Fanning
Keyword(s):  
Adenine Nucleotide ◽  
Nucleotide Binding ◽  
T Antigen ◽  
Sv40 T Antigen ◽  
Binding Properties ◽  
Nucleotide Analogues

Establishment and characterization of a SV40 T-antigen immortalized human bronchial epithelial cell line

1992 ◽  
Vol 28 (7-8) ◽  
pp. 461-464 ◽  
Author(s):  
Joan H. Schiller ◽  
Chinghai Kao ◽  
Gerard Bittner ◽  
Chuck Harris ◽  
Terry D. Oberley ◽  
...  
Keyword(s):  
Epithelial Cell ◽  
Cell Line ◽  
Bronchial Epithelial Cell ◽  
T Antigen ◽  
Human Bronchial Epithelial Cell ◽  
Epithelial Cell Line ◽  
Sv40 T Antigen ◽  
Bronchial Epithelial

Partial purification and characterization of the SV40 T antigen

Cell ◽  
1974 ◽  
Vol 3 (1) ◽  
pp. 65-70 ◽  
Author(s):  
I.Craig Henderson ◽  
David M. Livingston
Keyword(s):  
T Antigen ◽  
Partial Purification ◽  
Sv40 T Antigen ◽  
Purification And Characterization

scholarly journals Expression of a homologously recombined erythopoietin-SV40 T antigen fusion gene in mouse liver: evidence for erythropoietin production by Ito cells

Blood ◽  
1994 ◽  
Vol 84 (6) ◽  
pp. 1823-1830 ◽  
Author(s):  
PH Maxwell ◽  
DJ Ferguson ◽  
MK Osmond ◽  
CW Pugh ◽  
A Heryet ◽  
...  
Keyword(s):  
Fusion Gene ◽  
Light And Electron Microscopy ◽  
T Antigen ◽  
Cell Populations ◽  
Cell Type ◽  
Sv40 T Antigen ◽  
Ito Cells ◽  
Ultrastructural Characterization ◽  
Space Of Disse

We have obtained transgenic mice in which an erythropoietin-SV40 virus T antigen fusion gene is homologously recombined into the native Epo locus. This gene is expressed in a tissue-specific manner closely resembling that of the native Epo gene. Immunohistochemical detection of SV40 T antigen has been used to characterize the hepatic cell populations expressing the transgene. In mice stimulated by anaemia or hypobaric hypoxia, SV40 T antigen was demonstrated in two liver cell populations: a subset of hepatocytes and a nonparenchymal cell type. Immunohistochemical and ultrastructural characterization of these cells by light and electron microscopy showed the nonparenchymal cell type to be the Ito cells, which lie in a persinusoidal position within the space of Disse. We therefore conclude that Ito cells are the nonhepatocytic source of liver Epo production. These cells show many similarities to the Epo-producing fibroblastoid interstitial cells of the kidney.

Establishment and Characterization of SV40 T-Antigen Immortalized Human Liver Cells

1991 ◽  
pp. 323-332 ◽  
Author(s):  
Masayoshi Namba ◽  
Yoshio Kano ◽  
Li-yan Bai ◽  
Koichiro Mihara ◽  
Masahiro Miyazaki
Keyword(s):  
Human Liver ◽  
Liver Cells ◽  
T Antigen ◽  
Sv40 T Antigen ◽  
Human Liver Cells

Ectopic expression of the human adenine nucleotide translocase, isoform 3 (ANT-3). Characterization of ligand binding properties

Mitochondrion ◽  
2005 ◽  
Vol 5 (1) ◽  
pp. 1-13 ◽  
Author(s):  
A.K. Carroll ◽  
W.R. Clevenger ◽  
T. Szabo ◽  
L.E. Ackermann ◽  
Y. Pei ◽  
...  
Keyword(s):  
Ligand Binding ◽  
Adenine Nucleotide ◽  
Ectopic Expression ◽  
Adenine Nucleotide Translocase ◽  
Binding Properties
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