Kinetic Study of the Concentration Dependence of the Mass Transfer Rate Coefficient in Anion-Exchange Chromatography of Bovine Serum Albumin

1999 ◽  
Vol 15 (4) ◽  
pp. 740-752 ◽  
Author(s):  
K. Miyabe ◽  
G. Guiochon
Keyword(s):  
Mass Transfer ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Kinetic Study ◽  
Bovine Serum ◽  
Transfer Rate ◽  
Rate Coefficient ◽  
Mass Transfer Rate ◽  
Anion Exchange Chromatography ◽  
Exchange Chromatography

scholarly journals REAGINIC ACTIVITY ASSOCIATED WITH IGG IMMUNOGLOBULIN

1966 ◽  
Vol 123 (5) ◽  
pp. 845-858 ◽  
Author(s):  
Robert T. Reid ◽  
Percy Minden ◽  
Richard S. Farr
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Anion Exchange ◽  
Bovine Serum ◽  
Anion Exchange Chromatography ◽  
Exchange Chromatography ◽  
Unique Property ◽  
Serum Fractions ◽  
Human Sera

Five human sera with reaginic activity to a number of allergens were fractionated using anion exchange chromatography. In each serum, fractions which contained detectable IgG and no detectable IgA had capacity to fix to skin and subsequently elicit a P-K reaction. Four of these sera had reaginic activity about equally distributed between fractions containing only IgG and fractions containing mixtures of IgG and IgA. A fifth serum contained reaginic activity to crystalline bovine serum albumin (BSA) and most of the activity was associated with the fraction which contained only IgG. This serum was extensively studied using a variety of techniques and it was confirmed that most of the reagin to BSA in this serum was in those fractions containing only IgG. Since reaginic activity can no longer be considered a unique property of IgA the implications of finding antibody with reaginic qualities in immunoglobulins other than IgA are discussed.

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