Natural Combinatorial Biosynthesis Involving Two Clusters for the Synthesis of Three Pyrrolamides in Streptomyces netropsis

2014 ◽  
Vol 10 (2) ◽  
pp. 601-610 ◽  
Author(s):  
Audrey Vingadassalon ◽  
Florence Lorieux ◽  
Maud Juguet ◽  
Géraldine Le Goff ◽  
Claude Gerbaud ◽  
...  
Keyword(s):  
Combinatorial Biosynthesis ◽  
Streptomyces Netropsis

Expanding the Natural Products Heterologous Expression Repertoire in the Model Cyanobacterium Anabaena sp. Strain PCC 7120: Production of Pendolmycin and Teleocidin B-4

2019 ◽  
Author(s):  
Patrick Videau ◽  
Kaitlyn Wells ◽  
Arun Singh ◽  
Jessie Eiting ◽  
Philip Proteau ◽  
...  
Keyword(s):  
Natural Products ◽  
Genome Mining ◽  
Gene Clusters ◽  
Combinatorial Biosynthesis ◽  
Test Case ◽  
Biosynthetic Gene ◽  
Biosynthetic Gene Clusters ◽  
Cyanobacterium Anabaena ◽  
Anabaena Sp ◽  
Pcc 7120

Cyanobacteria are prolific producers of natural products and genome mining has shown that many orphan biosynthetic gene clusters can be found in sequenced cyanobacterial genomes. New tools and methodologies are required to investigate these biosynthetic gene clusters and here we present the use of <i>Anabaena </i>sp. strain PCC 7120 as a host for combinatorial biosynthesis of natural products using the indolactam natural products (lyngbyatoxin A, pendolmycin, and teleocidin B-4) as a test case. We were able to successfully produce all three compounds using codon optimized genes from Actinobacteria. We also introduce a new plasmid backbone based on the native <i>Anabaena</i>7120 plasmid pCC7120ζ and show that production of teleocidin B-4 can be accomplished using a two-plasmid system, which can be introduced by co-conjugation.

Expanding the Scope of Aromatic Polyketides by Combinatorial Biosynthesis

2003 ◽  
Vol 6 (6) ◽  
pp. 501-512 ◽  
Author(s):  
J. Kantola ◽  
T. Kunnari ◽  
P. Mantsala ◽  
K. Ylihonko
Keyword(s):  
Combinatorial Biosynthesis ◽  
Aromatic Polyketides

Accessing Natural Products by Combinatorial Biosynthesis

2004 ◽  
Vol 2004 (225) ◽  
pp. pe14-pe14 ◽  
Author(s):  
B. Shen
Keyword(s):  
Natural Products ◽  
Combinatorial Biosynthesis

Combinatorial biosynthesis in symbiotic bacteria

2006 ◽  
Vol 2 (12) ◽  
pp. 661-662 ◽  
Author(s):  
Jörn Piel
Keyword(s):  
Combinatorial Biosynthesis ◽  
Symbiotic Bacteria

scholarly journals The Combinatorial Biosynthesis of “Unnatural” Products with Polyketides

2018 ◽  
Vol 24 (6) ◽  
pp. 501-512 ◽  
Author(s):  
Chuanbo Zhang ◽  
Di Ke ◽  
Yuejiao Duan ◽  
Wenyu Lu
Keyword(s):  
Combinatorial Biosynthesis ◽  
Unnatural Products

Combinatorial biosynthesis for drug development

2007 ◽  
Vol 10 (3) ◽  
pp. 238-245 ◽  
Author(s):  
Hugo G Menzella ◽  
Christopher D Reeves
Keyword(s):  
Drug Development ◽  
Combinatorial Biosynthesis

scholarly journals Probing the promiscuity of ent-kaurene oxidases via combinatorial biosynthesis

2016 ◽  
Vol 113 (9) ◽  
pp. 2526-2531 ◽  
Author(s):  
Sibongile Mafu ◽  
Meirong Jia ◽  
Jiachen Zi ◽  
Dana Morrone ◽  
Yisheng Wu ◽  
...  
Keyword(s):  
Natural Products ◽  
Metabolic Engineering ◽  
Substrate Specificity ◽  
Plant Hormone ◽  
Structural Similarity ◽  
Combinatorial Biosynthesis ◽  
Underlying Structure ◽  
Engineering System ◽  
Insight Into

The substrate specificity of enzymes from natural products’ metabolism is a topic of considerable interest, with potential biotechnological use implicit in the discovery of promiscuous enzymes. However, such studies are often limited by the availability of substrates and authentic standards for identification of the resulting products. Here, a modular metabolic engineering system is used in a combinatorial biosynthetic approach toward alleviating this restriction. In particular, for studies of the multiply reactive cytochrome P450, ent-kaurene oxidase (KO), which is involved in production of the diterpenoid plant hormone gibberellin. Many, but not all, plants make a variety of related diterpenes, whose structural similarity to ent-kaurene makes them potential substrates for KO. Use of combinatorial biosynthesis enabled analysis of more than 20 such potential substrates, as well as structural characterization of 12 resulting unknown products, providing some insight into the underlying structure–function relationships. These results highlight the utility of this approach for investigating the substrate specificity of enzymes from complex natural products’ biosynthesis.

Combinatorial Biosynthesis, Metabolic Engineering and Mutasynthesis for the Generation of New Aminocoumarin Antibiotics

2008 ◽  
Vol 8 (8) ◽  
pp. 667-679 ◽  
Author(s):  
L. Heide ◽  
B. Gust ◽  
C. Anderle ◽  
S.-M. Li
Keyword(s):  
Metabolic Engineering ◽  
Combinatorial Biosynthesis
Sign in / Sign up

Export Citation Format

Share Document