Studies of mixed surfactant microemulsion systems: Brij 35 with Tween 20 and sodium dodecyl sulfate

1995 ◽  
Vol 99 (40) ◽  
pp. 14778-14783 ◽  
Author(s):  
S. Ajith ◽  
Kumar Rakshit
1997 ◽  
Vol 35 (7) ◽  
pp. 123-130 ◽  
Author(s):  
J. C. Liu ◽  
P. S. Chang

The solubility of chlorophenols as affected by surfactant was investigated. Three kinds of surfactant, sodium dodecyl sulfate, Triton X-100, and Brij 35, were utilized. The solubilization of chlorophenols by surfactant follows the order of 2,4,6-trichlorophenol > 2,4-dichlorophenol > 2,6-dichlorophenol > 2-chlorophenol; and the critical micelle concentration is an important index. The adsorption reactions of 2,4-dichlorophenol and 2,4,6- trichlorophenol onto hydrous montmorillonite in the presence of surfactant were examined. The presence of surfactant decreased the adsorption of chlorophenols significantly. The roles of hydrophobicity of chlorophenols in solubilization and adsorption behaviors are discussed.


2020 ◽  
Vol 83 (9) ◽  
pp. 1488-1494 ◽  
Author(s):  
EWA PIETRYSIAK ◽  
JULIANNE M. KUMMER ◽  
INES HANRAHAN ◽  
GIRISH M. GANJYAL

ABSTRACT This study investigated the decontamination efficacy of washing treatments for whole fresh apples by using washes containing surfactants, lauric arginate, sodium dodecyl sulfate, and Tween 20, combined with peracetic acid (PAA), followed by hot air impingement drying. Whole fresh apples of selected varieties (Gala and Granny Smith) were inoculated with Listeria innocua (7 log CFU/mL) by using a dipping method, and then dried and subjected to wash treatments with selected washing solutions (H2O, PAA, PAA–lauric arginate, PAA–sodium dodecyl sulfate, and PAA–Tween 20), followed by hot air impingement drying at two different temperature and time conditions, 93°C for 60 s or 121°C for 25 s. The H2O and PAA wash followed by hot air impingement drying led to a maximum 1.5-log reduction of L. innocua on the fruit surface. Adding surfactants increased the effectiveness of washing and drying treatments, resulting in an approximate 2.2-log reduction. Surfactants increased the spreadability and evaporation rate of the washing solutions. Posttreatment changes in apple firmness were assessed during a 21-day storage at 4 and 21°C. The hot air impingement drying had no adverse effect on the firmness of the apples and did not show any further reduction in L. innocua. Washing apples with solutions containing surfactants combined with PAA followed by hot air impingement drying helped to reduce the microbial loads to some extent and may help to reduce drying times significantly. HIGHLIGHTS


2004 ◽  
Vol 67 (3) ◽  
pp. 524-532 ◽  
Author(s):  
ROSELY A. B. NICHOLS ◽  
HUW V. SMITH

The numerous published methods for extracting DNA from Cryptosporidium oocysts for PCR identify the lack of an optimized standard method for clinical, environmental, and public health investigations of cryptosporidiosis. A method that maximizes DNA extraction reliably, particularly from small numbers of partially purified or purified oocysts present in mineral waters and environmental samples, is required. We describe a maximized method for liberating DNA from Cryptosporidium parvum oocysts by 15 cycles of freezing (liquid nitrogen) and thawing (65°C) in lysis buffer containing sodium dodecyl sulfate. The inhibitory effects of sodium dodecyl sulfate are abrogated by the addition of Tween 20 to the PCR reaction. We tested seven different C. parvum oocyst isolates, consistently detecting fewer than five oocysts following direct PCR amplification of a segment of the 18S rRNA gene. Older oocysts, which were more refractory to freeze-thawing, were disrupted effectively. A single oocyst in each of two mineral water concentrates was detected by both microscopy and PCR/Southern blotting. We recommend 15 cycles of freeze-thawing, with thawing at 65°C in lysis buffer, to maximize oocyst disruption and DNA extraction, particularly when isolate history and oocyst age are unknown. Both the DNA extraction method and the PCR described can be used for clinical, environmental, and public health investigations of cryptosporidiosis.


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