Nuclear magnetic resonance study of the conformation of .beta.-cyanuric acid riboside. Further evidence for the anti rotamer in pyrimidine nucleosides

1971 ◽  
Vol 93 (14) ◽  
pp. 3468-3470 ◽  
Author(s):  
Ian C. P. Smith ◽  
H. Dugas ◽  
B. J. Blackburn ◽  
R. K. Robins ◽  
Roxanne Deslauriers

1974 ◽  
Vol 52 (6) ◽  
pp. 924-929 ◽  
Author(s):  
George Kotowycz

The influence of paramagnetic Cu2+ ions on the proton decoupled 13C n.m.r. spectra of pyrimidine nucleosides and nucleotides has been studied. For 5′-CMP, the C5 resonance is broadened first on the addition of Cu2+ ions followed by the C2, C4, and C1′ resonances. From a comparison of the transverse and longitudinal relaxation rates of the base carbon nuclei due to the presence of Cu2+ ions, binding of Cu2+ to the N3 nitrogen of 5′-CMP is predicted. A similar broadening behavior is observed for 5′-UMP, 5′-TMP, cytidine, and uridine. This indicates that the Cu2+ ion is located near the N3 nitrogen in these Cu2+-nucleoside and -nucleotide complexes.







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