Selection of Human Metalloantibodies from a Combinatorial Phage Single-Chain Antibody Library

1999 ◽  
Vol 121 (27) ◽  
pp. 6517-6518 ◽  
Author(s):  
Changshou Gao ◽  
Oliver Brümmer ◽  
Shenlan Mao ◽  
Kim D. Janda
Keyword(s):  
Antibody Library ◽  
Single Chain ◽  
Single Chain Antibody ◽  
Selection Of

Construction of an immunized rabbit phage display antibody library for screening microcystin-LR high sensitive single-chain antibody

2019 ◽  
Vol 123 ◽  
pp. 369-378 ◽  
Author(s):  
Chongxin Xu ◽  
Wenjie Miao ◽  
Yan He ◽  
Yao Zu ◽  
Xiaoqin Liu ◽  
...  
Keyword(s):  
Phage Display ◽  
Antibody Library ◽  
Single Chain ◽  
Single Chain Antibody ◽  
Phage Display Antibody

Screening and Preliminary Verification of a Phage Display Single-Chain Antibody Library Against Coal Workers’ Pneumoconiosis

2016 ◽  
Vol 58 (12) ◽  
pp. 1264-1269 ◽  
Author(s):  
Guang-Hui Zhang ◽  
Li Li ◽  
Changfu Hao ◽  
Jing-Chao Ren ◽  
Hongyi Zhang ◽  
...  
Keyword(s):  
Phage Display ◽  
Antibody Library ◽  
Single Chain ◽  
Single Chain Antibody ◽  
Coal Workers Pneumoconiosis ◽  
Coal Workers

scholarly journals Phage antibody library screening for the selection of novel high-affinity human single-chain variable fragment against gastrin receptor: an in silico and in vitro study

2019 ◽  
Vol 27 (1) ◽  
pp. 21-34 ◽  
Author(s):  
Sepideh Jalilzadeh-Razin ◽  
Malihe Mantegi ◽  
Mohammad R. Tohidkia ◽  
Yaghub Pazhang ◽  
Mohammad M. Pourseif ◽  
...  
Keyword(s):  
In Silico ◽  
In Vitro Study ◽  
Library Screening ◽  
Single Chain Variable Fragment ◽  
Antibody Library ◽  
Single Chain ◽  
High Affinity ◽  
Phage Antibody Library ◽  
Selection Of

scholarly journals Phage display and kinetic selection of antibodies that specifically inhibit amyloid self-replication

2017 ◽  
Vol 114 (25) ◽  
pp. 6444-6449 ◽  
Author(s):  
Anna Munke ◽  
Jonas Persson ◽  
Tanja Weiffert ◽  
Erwin De Genst ◽  
Georg Meisl ◽  
...  
Keyword(s):  
Phage Display ◽  
Amyloid Fibrils ◽  
Amyloid Β ◽  
Amyloid Β Peptide ◽  
Nucleation Process ◽  
Single Chain ◽  
Secondary Nucleation ◽  
Single Chain Antibody ◽  
Phage Display Libraries ◽  
Selection Of

The aggregation of the amyloid β peptide (Aβ) into amyloid fibrils is a defining characteristic of Alzheimer’s disease. Because of the complexity of this aggregation process, effective therapeutic inhibitors will need to target the specific microscopic steps that lead to the production of neurotoxic species. We introduce a strategy for generating fibril-specific antibodies that selectively suppress fibril-dependent secondary nucleation of the 42-residue form of Aβ (Aβ42). We target this step because it has been shown to produce the majority of neurotoxic species during aggregation of Aβ42. Starting from large phage display libraries of single-chain antibody fragments (scFvs), the three-stage approach that we describe includes (i) selection of scFvs with high affinity for Aβ42 fibrils after removal of scFvs that bind Aβ42 in its monomeric form; (ii) ranking, by surface plasmon resonance affinity measurements, of the resulting candidate scFvs that bind to the Aβ42 fibrils; and (iii) kinetic screening and analysis to find the scFvs that inhibit selectively the fibril-catalyzed secondary nucleation process in Aβ42 aggregation. By applying this approach, we have identified four scFvs that inhibit specifically the fibril-dependent secondary nucleation process. Our method also makes it possible to discard antibodies that inhibit elongation, an important factor because the suppression of elongation does not target directly the production of toxic oligomers and may even lead to its increase. On the basis of our results, we suggest that the method described here could form the basis for rationally designed immunotherapy strategies to combat Alzheimer’s and related neurodegenerative diseases.

scholarly journals Selection of Linkers for a Catalytic Single-chain Antibody Using Phage Display Technology

1996 ◽  
Vol 271 (26) ◽  
pp. 15682-15686 ◽  
Author(s):  
Ying Tang ◽  
Ning Jiang ◽  
Cushrow Parakh ◽  
Donald Hilvert
Keyword(s):  
Phage Display ◽  
Single Chain ◽  
Single Chain Antibody ◽  
Phage Display Technology ◽  
Display Technology ◽  
Selection Of

scholarly journals Construction of Murine Phage Antibody Library and Selection of Ricin-Specific Single-Chain Antibodies

IUBMB Life ◽  
1999 ◽  
Vol 48 (5) ◽  
pp. 513-517 ◽  
Author(s):  
Xinsheng Gao ◽  
Yixiu Huang ◽  
Shenggeng Zhu
Keyword(s):  
Antibody Library ◽  
Single Chain ◽  
Phage Antibody ◽  
Single Chain Antibodies ◽  
Phage Antibody Library ◽  
Selection Of

scholarly journals Use of a Single-Chain Antibody Library for Ovarian Cancer Biomarker Discovery

2010 ◽  
Vol 9 (7) ◽  
pp. 1449-1460 ◽  
Author(s):  
Arturo B. Ramirez ◽  
Christian M. Loch ◽  
Yuzheng Zhang ◽  
Yan Liu ◽  
Xiaohong Wang ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Biomarker Discovery ◽  
Cancer Biomarker ◽  
Antibody Library ◽  
Single Chain ◽  
Single Chain Antibody

Construction of Murine Phage Antibody Library and Selection of Ricin-Specific Single-Chain Antibodies

IUBMB Life ◽  
1999 ◽  
Vol 48 (5) ◽  
pp. 513-517
Author(s):  
Xinsheng Gao ◽  
Yixiu Huang ◽  
Shenggeng Zhu
Keyword(s):  
Antibody Library ◽  
Single Chain ◽  
Phage Antibody ◽  
Single Chain Antibodies ◽  
Phage Antibody Library ◽  
Selection Of
Sign in / Sign up

Export Citation Format

Share Document