scholarly journals Functional Water Wires Catalyze Long-Range Proton Pumping in the Mammalian Respiratory Complex I

2020 ◽  
Vol 142 (52) ◽  
pp. 21758-21766
Author(s):  
Michael Röpke ◽  
Patricia Saura ◽  
Daniel Riepl ◽  
Maximilian C. Pöverlein ◽  
Ville R. I. Kaila
Keyword(s):  
Long Range ◽  
Complex I ◽  
Proton Pumping ◽  
Respiratory Complex ◽  
Respiratory Complex I

scholarly journals Energetics and dynamics of long-range electron transfer in respiratory complex I

2018 ◽  
Vol 1859 ◽  
pp. e42
Author(s):  
Michael Röpke ◽  
Ana P. Gamiz-Hernandez ◽  
Alexander Jussupow ◽  
Mikael P. Johansson ◽  
Ville R.I. Kaila
Keyword(s):  
Electron Transfer ◽  
Long Range ◽  
Complex I ◽  
Respiratory Complex ◽  
Respiratory Complex I

Mammalian Respiratory Complex I Through the Lens of Cryo-EM

2019 ◽  
Vol 48 (1) ◽  
pp. 165-184 ◽  
Author(s):  
Ahmed-Noor A. Agip ◽  
James N. Blaza ◽  
Justin G. Fedor ◽  
Judy Hirst
Keyword(s):  
Complex I ◽  
Catalytic Mechanism ◽  
Structural Data ◽  
Proton Pumping ◽  
Nadh:Ubiquinone Oxidoreductase ◽  
Respiratory Complex ◽  
Data Collection Strategy ◽  
Structural Work ◽  
Membrane Bound ◽  
Respiratory Complex I

Single-particle electron cryomicroscopy (cryo-EM) has led to a revolution in structural work on mammalian respiratory complex I. Complex I (mitochondrial NADH:ubiquinone oxidoreductase), a membrane-bound redox-driven proton pump, is one of the largest and most complicated enzymes in the mammalian cell. Rapid progress, following the first 5-Å resolution data on bovine complex I in 2014, has led to a model for mouse complex I at 3.3-Å resolution that contains 96% of the 8,518 residues and to the identification of different particle classes, some of which are assigned to biochemically defined states. Factors that helped improve resolution, including improvements to biochemistry, cryo-EM grid preparation, data collection strategy, and image processing, are discussed. Together with recent structural data from an ancient relative, membrane-bound hydrogenase, cryo-EM on mammalian complex I has provided new insights into the proton-pumping machinery and a foundation for understanding the enzyme's catalytic mechanism.

scholarly journals The proton-pumping respiratory complex I of bacteria and mitochondria and its homologue in chloroplasts

FEBS Letters ◽  
1995 ◽  
Vol 367 (2) ◽  
pp. 107-111 ◽  
Author(s):  
Thorsten Friedrich ◽  
Klaus Steinmüller ◽  
Hanns Weiss
Keyword(s):  
Complex I ◽  
Proton Pumping ◽  
Respiratory Complex ◽  
Respiratory Complex I

scholarly journals How cardiolipin modulates the dynamics of respiratory complex I

2019 ◽  
Vol 5 (3) ◽  
pp. eaav1850 ◽  
Author(s):  
Alexander Jussupow ◽  
Andrea Di Luca ◽  
Ville R. I. Kaila
Keyword(s):  
Conformational Changes ◽  
Complex I ◽  
Specific Interaction ◽  
Proton Pumping ◽  
Respiratory Enzymes ◽  
Respiratory Complex ◽  
Respiratory Chains ◽  
Membrane Bound ◽  
Respiratory Complex I ◽  
Structure And Activity

Cardiolipin modulates the activity of membrane-bound respiratory enzymes that catalyze biological energy transduction. The respiratory complex I functions as the primary redox-driven proton pump in mitochondrial and bacterial respiratory chains, and its activity is strongly enhanced by cardiolipin. However, despite recent advances in the structural biology of complex I, cardiolipin-specific interaction mechanisms currently remain unknown. On the basis of millisecond molecular simulations, we suggest that cardiolipin binds to proton-pumping subunits of complex I and induces global conformational changes that modulate the accessibility of the quinone substrate to the enzyme. Our findings provide key information on the coupling between complex I dynamics and activity and suggest how biological membranes modulate the structure and activity of proteins.

scholarly journals Coupling of quinone dynamics to proton pumping in respiratory complex I

2020 ◽  
Vol 1861 (12) ◽  
pp. 148287 ◽  
Author(s):  
Outi Haapanen ◽  
Marco Reidelbach ◽  
Vivek Sharma
Keyword(s):  
Complex I ◽  
Proton Pumping ◽  
Respiratory Complex ◽  
Respiratory Complex I

scholarly journals Towards a mechanistic model for redox-driven proton pumping in respiratory complex I

2016 ◽  
Vol 1857 ◽  
pp. e40
Author(s):  
Ville R.I. Kaila ◽  
Andrea di Luca ◽  
Ana P. Gamiz-Hernandez ◽  
Alexander Jussupow ◽  
Mikael P. Johansson ◽  
...  
Keyword(s):  
Complex I ◽  
Mechanistic Model ◽  
Proton Pumping ◽  
Respiratory Complex ◽  
Respiratory Complex I
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