Isopiestic determination of the osmotic and relative activity coefficients in barium chloride-zinc chloride-water at 25 .degree.C

1987 ◽  
Vol 32 (2) ◽  
pp. 205-210 ◽  
Author(s):  
Chai Fu Pan ◽  
William J. Argersinger

1990 ◽  
Vol 94 (19) ◽  
pp. 7706-7710 ◽  
Author(s):  
Rabindra N. Roy ◽  
Susan A. Rice ◽  
Kathleen M. Vogel ◽  
Lakshmi N. Roy ◽  
Frank J. Millero




1965 ◽  
Vol 20 (6) ◽  
pp. 1332-1336 ◽  
Author(s):  
Edward W. Moore ◽  
James W. Ross

In the investigation of numerous physiological phenomena it is the activity of an ion species which is desired, rather than stoichiometric concentration. The calculation of mean ionic activity from known concentration data requires accurate activity coefficients (ggr). This report concerns the determination of ggrNaCl and ggrCaCl2 in mixed NaCl-CaCl2 solutions by potentiometric measurement with a sodium-selective glass electrode-Ag/AgCl electrode system over the ionic strength range 0.05–0.5 m. Log ggrNaCl varied linearly, at constant total ionic strength, with the ionic strength of CaCl2 in the mixture, in accordance with Harned's rule. From data thus obtained, ggrCaCl2 coefficients in such mixed solutions have been calculated and compared with values calculated from published osmotic data. Resulting activity coefficient curves for ggrCaCl2 are presented over the concentration range encountered in serum and other extracellular fluids. Note: (With the Technical Assistance of Leonard Kaye and Leonard L. Anderson) glass electrodes; ion interaction; electrolyte metabolism; Harned's rule; membrane transport; osmotic coefficients Submitted on March 11, 1965



Author(s):  
Ninoek Indriati ◽  
Arifah Kusmarwati ◽  
Irma Hermana

Previous study of bacteriocin production on laboratory scale (100 mL) that used MRS broth medium produced unstable activity of bacteriocin. Therefore, this study aims to determine the optimum growth conditions and media for production of bacteriocin. Bacteria used in this research was a lactic acid bacteria (LAB) Lactococcus lactis ssp. lactis CN1.10a  isolated from rusip, a traditional Bangkanese fermented fish product.The bacteria was first cultivated for subsequent use of bacteriocins production on intermediate scale (2L). Followed by the optimization of temperature, pH and medium for the bacteriocin production, determination of cell growth curve, bacteriocin production curve, bacteriocin activity on that scale, and also stability of bacteriocin during storage.The results showed that the optimum temperature and pH for the growth of producer cell were 28°C and pH 6. The greatest activity of bacteriocin was produced on CM medium (1% sucrose, 0,45% peptone, 1% yeast extract, 2,84% KH2PO4, 0,2% NaCl and 0,02% MgSO4.7H20) in addition of sucrose as carbohydrate source. Based on the growth curve performedon CM medium with KH2PO4, the L. Lactis ssp lactis CN1.10a was relatively stable up to 48 hours. Bacteriocin produced by the cell was  8000 AU/mlat24th hour.Bacteriocin  was relatively stable when stored at -20°C for 1month with a relative activity of 69,4%.



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