Purification and Partial Characterization ofLactobacillusSpecies SK007 Lactate Dehydrogenase (LDH) Catalyzing Phenylpyruvic Acid (PPA) Conversion into Phenyllactic Acid (PLA)

2008 ◽  
Vol 56 (7) ◽  
pp. 2392-2399 ◽  
Author(s):  
Xingfeng Li ◽  
Bo Jiang ◽  
Beilei Pan ◽  
Wanmeng Mu ◽  
Tao Zhang
Keyword(s):  
Lactate Dehydrogenase ◽  
Partial Characterization ◽  
Phenylpyruvic Acid ◽  
Phenyllactic Acid

scholarly journals Enzymological characterization of a novel d-lactate dehydrogenase from Lactobacillus rossiae and its application in d-phenyllactic acid synthesis

3 Biotech ◽  
2020 ◽  
Vol 10 (3) ◽  
Author(s):  
Xi Luo ◽  
Yingying Zhang ◽  
Fengwei Yin ◽  
Gaowei Hu ◽  
Qiang Jia ◽  
...  
Keyword(s):  
Lactate Dehydrogenase ◽  
Acid Synthesis ◽  
Phenyllactic Acid

Amino group requirement for in vitro intestinal transport of amino acids

1962 ◽  
Vol 202 (1) ◽  
pp. 171-173 ◽  
Author(s):  
Richard P. Spencer ◽  
Ted M. Bow ◽  
Mary Anne Markulis
Keyword(s):  
Amino Acids ◽  
Acetic Acid ◽  
Cinnamic Acid ◽  
Concentration Gradient ◽  
Anthranilic Acid ◽  
Intestinal Transport ◽  
Amino Group ◽  
Phenylpyruvic Acid ◽  
Phenyllactic Acid

The amino group requirement for transintestinal transport of amino acids against a concentration gradient was investigated using hamster everted intestinal sacs. Although glycine (5 x 10–3 m) was transported against a concentration gradient, acetic acid was not. Similarly, l-phenylalanine was transported, whereas phenylpyruvic acid, phenylpropionic acid, phenyllactic acid, and cinnamic acid were not. l-Tryptophan was transported, but indolyllactic acid was not. The amino group was thus essential for transport by this system. n-Methylglycine and l-proline were accumulated from mucosa to serosa against a concentration gradient. Hence, one hydrogen of the amino group can be replaced. However, n-phenylglycine was not accumulated across these preparations, suggesting that the moiety replacing the amino hydrogen can not be sterically bulky. α-l-Alanine was transported against a concentration gradient from mucosa to serosa, but ß-alanine was not. This is in contrast to other systems which accumulate ß-alanine against a concentration gradient. Anthranilic acid, with the amino group in a relative ß position, was also not accumulated across everted intestinal sacs.

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