Glabridin Protects Paraoxonase 1 from Linoleic Acid Hydroperoxide Inhibition via Specific Interaction: A Fluorescence-Quenching Study

2012 ◽  
Vol 60 (14) ◽  
pp. 3679-3685 ◽  
Author(s):  
Dana Atrahimovich ◽  
Jacob Vaya ◽  
Hagai Tavori ◽  
Soliman Khatib
Keyword(s):  
Linoleic Acid ◽  
Fluorescence Quenching ◽  
Specific Interaction ◽  
Paraoxonase 1 ◽  
Linoleic Acid Hydroperoxide ◽  
Acid Hydroperoxide

scholarly journals Increased Levels of Human Carotid Lesion Linoleic Acid Hydroperoxide in Symptomatic and Asymptomatic Patients Is Inversely Correlated with Serum HDL and Paraoxonase 1 Activity

2012 ◽  
Vol 2012 ◽  
pp. 1-9 ◽  
Author(s):  
Elad Cohen ◽  
Michael Aviram ◽  
Soliman Khatib ◽  
Asaf Rabin ◽  
Dalit Mannheim ◽  
...  
Keyword(s):  
Linoleic Acid ◽  
Specific Inhibitor ◽  
Paraoxonase 1 ◽  
Pon1 Activity ◽  
Oxidized Lipids ◽  
Linoleic Acid Hydroperoxide ◽  
Asymptomatic Patients ◽  
Acid Hydroperoxide ◽  
Serum Pon1 Activity ◽  
Human Carotid

Human carotid plaque components interact directly with circulating blood elements and thus they might affect each other. We determined plaque paraoxonase1 (PON1) hydrolytic-catalytic activity and compared plaque and blood levels of lipids, HDL, PON1, and HbA1c, as well as plaque-oxidized lipids in symptomatic and asymptomatic patients. Human carotid plaques were obtained from symptomatic and asymptomatic patients undergoing routine endarterectomy, and the lesions were ground and extracted for PON activity and lipid content determinations. Plaque PONs preserved paraoxonase, arylesterase, and lactonase activities. The PON1-specific inhibitor 2-hydroxyquinoline almost completely inhibited paraoxonase and lactonase activities, while only moderately inhibiting arylesterase activity. Oxysterol and triglyceride levels in plaques from symptomatic and asymptomatic patients did not differ significantly, but plaques from symptomatic patients had significantly higher (135%) linoleic acid hydroperoxide (LA-13OOH) levels. Their serum PON1 activity, cholesterol and triglyceride levels did not differ significantly, but symptomatic patients had significantly lower (28%) serum HDL levels and higher (18%) HbA1c levels. Thus LA-13OOH, a major atherogenic plaque element, showed significant negative correlations with serum PON1 activity and HDL levels, and a positive correlation with the prodiabetic atherogenic HbA1c. Plaque PON1 retains its activity and may decrease plaque atherogenicity by reducing specific oxidized lipids (e.g., LA-13OOH). The inverse correlation between plaque LA-13OOH level and serum HDL level and PON1 activity suggests a role for serum HDL and PON1 in LA-13OOH accumulation.

Human carotid lesion linoleic acid hydroperoxide inhibits paraoxonase 1 (PON1) activity via reaction with PON1 free sulfhydryl cysteine 284

2011 ◽  
Vol 50 (1) ◽  
pp. 148-156 ◽  
Author(s):  
Hagai Tavori ◽  
Michael Aviram ◽  
Soliman Khatib ◽  
Ramadan Musa ◽  
Dalit Mannheim ◽  
...  
Keyword(s):  
Linoleic Acid ◽  
Paraoxonase 1 ◽  
Pon1 Activity ◽  
Linoleic Acid Hydroperoxide ◽  
Carotid Lesion ◽  
Acid Hydroperoxide ◽  
Human Carotid

scholarly journals Interaction between Mitochondrial Cytochromes and Linoleic Acid Hydroperoxide

1982 ◽  
Vol 69 (6) ◽  
pp. 1308-1314 ◽  
Author(s):  
Jacques Dupont ◽  
Pierre Rustin ◽  
Claude Lance
Keyword(s):  
Linoleic Acid ◽  
Linoleic Acid Hydroperoxide ◽  
Acid Hydroperoxide ◽  
Mitochondrial Cytochromes

Homolytic decomposition of linoleic acid hydroperoxide: Identification of fatty acid products

Lipids ◽  
1974 ◽  
Vol 9 (9) ◽  
pp. 696-706 ◽  
Author(s):  
H. W. Gardner ◽  
R. Kleiman ◽  
D. Weisleder
Keyword(s):  
Fatty Acid ◽  
Linoleic Acid ◽  
Linoleic Acid Hydroperoxide ◽  
Homolytic Decomposition ◽  
Acid Hydroperoxide

scholarly journals Flavonoids Suppress the Cytotoxicity of Linoleic Acid Hydroperoxide toward PC12 Cells

2002 ◽  
Vol 25 (8) ◽  
pp. 1093-1096 ◽  
Author(s):  
Naoko Sasaki ◽  
Takeshi Toda ◽  
Takao Kaneko ◽  
Naomichi Baba ◽  
Mitsuyoshi Matsuo
Keyword(s):  
Linoleic Acid ◽  
Pc12 Cells ◽  
Linoleic Acid Hydroperoxide ◽  
Acid Hydroperoxide

Synthesis of linoleic acid hydroperoxide using immobilized lipoxygenase in polyacrylamide gel

1996 ◽  
Vol 59 (3) ◽  
pp. 309-318 ◽  
Author(s):  
M. Carmen Pinto ◽  
Pedro Macías
Keyword(s):  
Linoleic Acid ◽  
Polyacrylamide Gel ◽  
Linoleic Acid Hydroperoxide ◽  
Acid Hydroperoxide

Intracellular mechanisms for the decomposition of a lipid peroxide. II. Decomposition of a lipid peroxide by subcellular fractions

1969 ◽  
Vol 47 (5) ◽  
pp. 493-499 ◽  
Author(s):  
P. J. O'Brien ◽  
C. Little
Keyword(s):  
Linoleic Acid ◽  
Glutathione Peroxidase ◽  
Ph Dependence ◽  
Lipid Peroxide ◽  
Subcellular Fractions ◽  
Radical Mechanism ◽  
Complexing Agents ◽  
Linoleic Acid Hydroperoxide ◽  
Hydrogen Donors ◽  
Acid Hydroperoxide

The properties of subcellular fractions of rat liver in catalyzing the decomposition of linoleic acid hydroperoxide have been compared with those of transition salts, heme compounds, and nucleophiles. The properties compared included the range of products produced, the pH dependence of the reaction, and the effects of metal-complexing agents, inhibitors, and hydrogen donors. It was concluded that the decomposition of the hydroperoxide in the liver cell was due principally to reaction with the intracellular nucleophile glutathione by a mechanism catalyzed by the enzyme glutathione peroxidase. In the absence of glutathione, however, both the mitochondrial and microsomal fractions decomposed the hydroperoxide presumably by a radical mechanism probably involving the cytochromes.

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