Simultaneous use of empirical and semiempirical substituent parameters as a new method of analysis of the ortho effect. Application in reactions via aryl anion intermediates

1985 ◽  
Vol 50 (7) ◽  
pp. 1045-1053 ◽  
Author(s):  
Pascual Segura
Keyword(s):  
New Method ◽  
Ortho Effect ◽  
Simultaneous Use

scholarly journals Reliable Detection of Dead Microbial Cells by Using Fluorescent Hydrazides

2009 ◽  
Vol 76 (5) ◽  
pp. 1674-1678 ◽  
Author(s):  
Claude Saint-Ruf ◽  
Corinne Cordier ◽  
J�rome M�gret ◽  
Ivan Matic
Keyword(s):  
Nucleic Acid ◽  
New Method ◽  
Cell Populations ◽  
Acid Dyes ◽  
Bacterial Populations ◽  
Microbial Cells ◽  
High Affinity ◽  
Reliable Detection ◽  
Simultaneous Use ◽  
Accurate Quantification

ABSTRACT We have developed a new method for accurate quantification of dead microbial cells. This technique employs the simultaneous use of fluorescent hydrazides and nucleic acid dyes. Fluorescent hydrazides allow detection of cells that cannot be detected with currently used high-affinity nucleic acid dyes. This is particularly important for nongrowing bacterial populations and for multicellular communities containing physiologically heterogeneous cell populations, such as colonies and biofilms.

Selective Sampling and Orientation of Non-Homogeneous Tissues

1968 ◽  
Vol 26 ◽  
pp. 98-99
Author(s):  
C. C. Clawson ◽  
L. W. Anderson ◽  
R. A. Good
Keyword(s):  
Electron Microscopy ◽  
Electron Microscope ◽  
Light Microscopy ◽  
Random Sampling ◽  
New Method ◽  
Microscope Examination ◽  
Small Areas ◽  
Selective Sampling ◽  
Large Numbers ◽  
Specific Orientation

Investigations which require electron microscope examination of a few specific areas of non-homogeneous tissues make random sampling of small blocks an inefficient and unrewarding procedure. Therefore, several investigators have devised methods which allow obtaining sample blocks for electron microscopy from region of tissue previously identified by light microscopy of present here techniques which make possible: 1) sampling tissue for electron microscopy from selected areas previously identified by light microscopy of relatively large pieces of tissue; 2) dehydration and embedding large numbers of individually identified blocks while keeping each one separate; 3) a new method of maintaining specific orientation of blocks during embedding; 4) special light microscopic staining or fluorescent procedures and electron microscopy on immediately adjacent small areas of tissue.

A new method for the determination of nitrates

1960 ◽  
Vol 23 ◽  
pp. 227-232 ◽  
Author(s):  
P WEST ◽  
G LYLES
Keyword(s):  
New Method
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