The limits of reaction of radioactive dicyclohexylcarbodiimide with amino groups during solid-phase peptide synthesis

1977 ◽  
Vol 42 (8) ◽  
pp. 1291-1295 ◽  
Author(s):  
R. B. Merrifield ◽  
B. F. Gisin ◽  
Anita N. Bach
Keyword(s):  
Peptide Synthesis ◽  
Solid Phase ◽  
Solid Phase Peptide Synthesis ◽  
Amino Groups

scholarly journals Role of capping in peptide synthesis

2020 ◽  
Vol 11 (4) ◽  
pp. 5225-5228
Author(s):  
Deepshikha Verma ◽  
Pillai V N R ◽  
Giriraj Tailor
Keyword(s):  
Peptide Synthesis ◽  
Solid Phase ◽  
Solid Phase Peptide Synthesis ◽  
Protecting Groups ◽  
Amino Groups ◽  
Peptide Chemistry ◽  
Fmoc Spps ◽  
Reliable Methods ◽  
Important Test

Protecting groups like Fmoc and coupling both steps are essential to monitoring the Fmoc SPPS (Solid Phase Peptide Synthesis) reaction completion. Reliable methods are used to detect the unreacted number of amino groups for monitoring these two essential reaction steps of coupling and cleavage. The ability to detect the complete coupling, incomplete coupling or failure of coupling we use many colour tests in the laboratory and based on this the Fmoc peptide chemistry allows the control of the completion of the Fmoc cleavage. The most important test used is the Kaiser test and highly recommended to monitor the coupling and cleavage steps. If the result of colour tests is positive after coupling, then the second coupling should be performed. Then again use the colour test to detect the level of coupling. If the result is still slightly positive, repeat coupling with the smaller modification of reagents such as used PyBOP instead of HOBT AND HOAT. These colour tests help in revealing the presence of unreacted amino-functional groups. Thus, we need to block these free N-terminal of amino- acids which help in avoiding the making of deletion of sequence.

Noninvasive continuous monitoring of solid-phase peptide synthesis by acid-base indicator

1988 ◽  
Vol 53 (11) ◽  
pp. 2542-2548 ◽  
Author(s):  
Viktor Krchňák ◽  
Josef Vágner ◽  
Pavel Šafář ◽  
Michal Lebl
Keyword(s):  
Peptide Synthesis ◽  
Continuous Monitoring ◽  
Solid Phase ◽  
Solid Phase Peptide Synthesis ◽  
Bromophenol Blue ◽  
Amino Groups ◽  
Acid Base ◽  
Qualitative And Quantitative ◽  
Quantitative Test ◽  
End Point

The course and the end point of the acylation of resin-bound amino groups in solid-phase peptide synthesis was monitored by a novel noninvasive qualitative and quantitative test based on the use of the acid-base indicator bromophenol blue.

scholarly journals Process Control in the Solid Phase Peptide Synthesis by Titration of Free Amino Groups.

1969 ◽  
Vol 23 ◽  
pp. 2906-2907 ◽  
Author(s):  
K. Brunfeldt ◽  
P. Roepstorff ◽  
J. Thomsen ◽  
D. Heinegård ◽  
Alexandru T. Balaban ◽  
...  
Keyword(s):  
Process Control ◽  
Peptide Synthesis ◽  
Free Amino ◽  
Solid Phase ◽  
Solid Phase Peptide Synthesis ◽  
Amino Groups

A Strategy for Thioamide Incorporation During Fmoc Solid-Phase Peptide Synthesis with Robust Stereochemical Integrity

2019 ◽  
Author(s):  
luis camacho III ◽  
Bryan J. Lampkin ◽  
Brett VanVeller
Keyword(s):  
Amino Acid ◽  
Functional Groups ◽  
Peptide Synthesis ◽  
Solid Phase ◽  
Solid Phase Peptide Synthesis ◽  
Side Chains ◽  
Amino Acid Side Chains ◽  
Peptide Elongation

We describe a method to protect the sensitive stereochemistry of the thioamide—in analogy to the protection of the functional groups of amino acid side chains—in order to preserve the thioamide moiety during peptide elongation.<br>

Analytical Methods for Solid Phase Peptide Synthesis

2004 ◽  
Vol 8 (4) ◽  
pp. 291-301 ◽  
Author(s):  
Giuseppina Sabatino ◽  
Mario Chelli ◽  
Alberto Brandi ◽  
Anna Papini
Keyword(s):  
Peptide Synthesis ◽  
Analytical Methods ◽  
Solid Phase ◽  
Solid Phase Peptide Synthesis
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