A New Measurement System for UV Resonance Raman Spectra of Large Proteins and Its Application to CytochromecOxidase†

2000 ◽  
Vol 104 (46) ◽  
pp. 10765-10774 ◽  
Author(s):  
M. Aki ◽  
T. Ogura ◽  
K. Shinzawa-Itoh ◽  
S. Yoshikawa ◽  
T. Kitagawa
Keyword(s):  
Raman Spectra ◽  
Measurement System ◽  
Resonance Raman ◽  
Large Proteins ◽  
Uv Resonance Raman ◽  
Resonance Raman Spectra

pH-Dependent UV Resonance Raman Spectra of Cytosine and Uracil

2009 ◽  
Vol 113 (20) ◽  
pp. 7392-7397 ◽  
Author(s):  
Brant E. Billinghurst ◽  
Sulayman A. Oladepo ◽  
Glen R. Loppnow
Keyword(s):  
Raman Spectra ◽  
Resonance Raman ◽  
Uv Resonance Raman ◽  
Ph Dependent ◽  
Resonance Raman Spectra

Amide V overtone assignment of a conformation-sensitive band in the UV resonance Raman spectra of peptides and proteins

1989 ◽  
Vol 111 (12) ◽  
pp. 4290-4294 ◽  
Author(s):  
Samuel Krimm ◽  
Sunho Song ◽  
Sanford A. Asher
Keyword(s):  
Raman Spectra ◽  
Resonance Raman ◽  
Uv Resonance Raman ◽  
Resonance Raman Spectra

UV Resonance Raman Spectra of Bacillus Spores

1992 ◽  
Vol 46 (2) ◽  
pp. 357-364 ◽  
Author(s):  
E. Ghiamati ◽  
R. Manoharan ◽  
W. H. Nelson ◽  
J. F. Sperry
Keyword(s):  
Aqueous Solutions ◽  
Bacillus Cereus ◽  
Raman Spectra ◽  
Dipicolinic Acid ◽  
Resonance Raman ◽  
Vegetative Cells ◽  
Resonance Enhancement ◽  
Uv Resonance Raman ◽  
Bacillus Spores ◽  
Resonance Raman Spectra

UV resonance Raman spectra of Bacillus cereus, Bacillus megaterium, and Bacillus subtilis endospores have been excited at 222.7,230.7,242.5, and 251.1 nm, and spectra have been compared with those of vegetative cells. The resonance Raman spectra of aqueous solutions of dipicolinic acid and calcium dipicolinate have been measured at the same wavelengths. Spectra of endospores and their corresponding germinated spores show only modest differences when excited at 222, 231, and 251 nm. However, very substantial differences appear when excitation occurs at 242 nm. Difference spectra obtained at 242 nm by subtracting spectra of germinated spores of Bacillus cereus from spectra of their corresponding endospores are attributed almost entirely to dipicolinate. Vegetative cells and endospores show large spectral dissimilarities at all exciting wavelengths. These spectral differences, which vary strongly with exciting wavelength, appear to be the result of large differences in the amounts and composition of proteins and nucleic acids, especially ribosomal RNA. The very substantial resonance enhancement of Raman spectra has been obtained from aqueous solutions of pure dipicolinic acid and of sodium and calcium dipicolinate salts, as well as spores at the various exciting wavelengths. The strong enhancement of dipicolinate spectra in spores, however, was noted only with 242-nm excitation. Consequently, only with 242-nm light was it possible to selectively and sensitively excite and study calcium dipicolinate in spores. Resonance enhancement of the dipicolinate spectra with 242-nm excitation appears due primarily to resonance interactions with n-π* electronic transitions associated with the pyridine ring and/or the carboxylate group.

Quantitative Treatment of UV Resonance Raman Spectra of Biological Molecules: Application to the Study of Membrane-Bound Proteins

1991 ◽  
Vol 45 (2) ◽  
pp. 272-278 ◽  
Author(s):  
Roman G. Efremov ◽  
Alexey V. Feofanov ◽  
Igor R. Nabiev
Keyword(s):  
Raman Spectra ◽  
Resonance Raman ◽  
Biological Molecules ◽  
Uv Resonance Raman ◽  
Membrane Bound ◽  
Resonance Raman Spectra ◽  
Quantitative Treatment

Dependence of Ethylguanidinium UV Resonance Raman Spectra on the Environment: Insights into the Arginine Sidechain in Peptide and Protein

2010 ◽  
Author(s):  
Zhenmin Hong ◽  
Sanford Asher ◽  
P. M. Champion ◽  
L. D. Ziegler
Keyword(s):  
Raman Spectra ◽  
Resonance Raman ◽  
Uv Resonance Raman ◽  
Resonance Raman Spectra

Time-resolved Absorption and UV Resonance Raman Spectra Reveal Stepwise Formation of T Quaternary Contacts in the Allosteric Pathway of Hemoglobin

2004 ◽  
Vol 340 (4) ◽  
pp. 843-856 ◽  
Author(s):  
Gurusamy Balakrishnan ◽  
Martin A Case ◽  
Alex Pevsner ◽  
Xiaojie Zhao ◽  
Charbel Tengroth ◽  
...  
Keyword(s):  
Raman Spectra ◽  
Resonance Raman ◽  
Time Resolved ◽  
Uv Resonance Raman ◽  
Resonance Raman Spectra ◽  
Allosteric Pathway ◽  
Stepwise Formation

UV resonance Raman spectra of bacteria, bacterial spores, protoplasts and calcium dipicolinate

1990 ◽  
Vol 11 (1) ◽  
pp. 1-15 ◽  
Author(s):  
R. Manoharan ◽  
E. Ghiamati ◽  
R.A. Dalterio ◽  
K.A. Britton ◽  
W.H. Nelson ◽  
...  
Keyword(s):  
Raman Spectra ◽  
Resonance Raman ◽  
Bacterial Spores ◽  
Uv Resonance Raman ◽  
Resonance Raman Spectra

Near-UV Circular Dichroism and UV Resonance Raman Spectra of Tryptophan Residues as a Structural Marker of Proteins

2013 ◽  
Vol 117 (32) ◽  
pp. 9343-9353 ◽  
Author(s):  
Shigenori Nagatomo ◽  
Masako Nagai ◽  
Takashi Ogura ◽  
Teizo Kitagawa
Keyword(s):  
Circular Dichroism ◽  
Raman Spectra ◽  
Resonance Raman ◽  
Tryptophan Residues ◽  
Uv Resonance Raman ◽  
Resonance Raman Spectra ◽  
Circular Dichroïsm
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