Non-Random Distribution of Certain Homologous Pairs of Normal Human Chromosomes in Metaphase

Nature ◽  
1962 ◽  
Vol 195 (4847) ◽  
pp. 1229-1230 ◽  
Author(s):  
LAWRENCE J. SCHNEIDERMAN ◽  
CEDRIC A. B. SMITH
Keyword(s):  
Random Distribution ◽  
Human Chromosomes ◽  
Normal Human

A Novel Technique for Observing the Internal Ultrastructure of Human Chromosomes with Known Karyotype

2008 ◽  
Vol 14 (4) ◽  
pp. 357-361 ◽  
Author(s):  
Mohammad Ghazizadeh ◽  
Yoshihiro Sasaki ◽  
Tatsuo Oguro ◽  
Shigeru Sato ◽  
Seiko Egawa ◽  
...  
Keyword(s):  
Metaphase Chromosome ◽  
Osmium Tetroxide ◽  
Human Lymphocytes ◽  
Fragile Sites ◽  
Human Chromosomes ◽  
Chromosome Research ◽  
Novel Technique ◽  
Transmission Electron ◽  
Comprehensive Information ◽  
Normal Human

Observation of the internal ultrastructure of human chromosomes by transmission electron microscopy (TEM) has frequently been attempted in spite of the difficulties in detaching metaphase chromosome spreads from the glass slide for further processing. In this study we have used a method in which metaphase chromosome spreads were prepared on a flexible thermoplastic membrane (ACLAR) film. To assess chromosome identity, a diamidino-phenylindole staining and karyotying was first done using a conventional cytogenetic system. The chromosome spreads were then fixed with 1% osmium tetroxide, stained with freshly prepared 2% tannic acid, dehydrated, and flat-embedded in epoxy resin. The resin sheet was easily detachable and carried whole chromosome spreads. By this method, TEM observation of chromosomes from normal human lymphocytes allowed a thorough examination of the ultrastructure of centromeres, telomeres, fragile sites, and other chromosomal regions. Various ultrastructural patterns including thick electron dense boundaries, less dense internal regions, and extended chromatin loops at the periphery of the chromosomes were discernible. Application of the present method to chromosome research is expected to provide comprehensive information on the internal ultrastructure of different chromosomal regions in relation to function.

Non-Random Distribution of Alu-Family DNA Repeats in Human Chromosomes

1990 ◽  
pp. 41-45
Author(s):  
L. V. Filatov ◽  
S. E. Mamayeva ◽  
N. V. Tomilin
Keyword(s):  
Random Distribution ◽  
Dna Repeats ◽  
Human Chromosomes

Non random distribution of lesions induced by deoxyribonuclease I in human chromosomes

1987 ◽  
Vol 1 (1) ◽  
pp. 19-24
Author(s):  
Fiorella Nuzzo ◽  
Alessandra Casati ◽  
Elena Raimondi
Keyword(s):  
Random Distribution ◽  
Human Chromosomes ◽  
Deoxyribonuclease I

Non-random distribution of Alu-family repeats in human chromosomes

1987 ◽  
Vol 12 (2) ◽  
pp. 117-121 ◽  
Author(s):  
L. V. Filatov ◽  
S. E. Mamayeva ◽  
N. V. Tomilin
Keyword(s):  
Random Distribution ◽  
Human Chromosomes

scholarly journals Microphotometrical image analysis of the subtelomeric region of T-banded endoreduplicated chromosomes of Chinese hamster ovary cells

1998 ◽  
Vol 21 (2) ◽  
pp. 219-225 ◽  
Author(s):  
Máximo E. Drets ◽  
Marcos Mendizábal
Keyword(s):  
Image Analysis ◽  
Chinese Hamster Ovary ◽  
Computer Graphic ◽  
Chinese Hamster Ovary Cells ◽  
Chinese Hamster ◽  
Human Chromosomes ◽  
Ovary Cells ◽  
Normal Human ◽  
Graphic Image ◽  
Chromatin Density

Microphotometrical scanning and computer graphic image analysis were carried out to detect the distribution of chromatin densities in subtelomeric segments of T-banded endoreduplicated chromosomes of Chinese hamster ovary (CHO) cells. Chromatin density patterns detected with this method were similar to those previously found in CHO and normal human chromosomes. The highest chromatin densities were considered as marker segments which led to the detection of reciprocal changes of position in endoreduplicated chromosomes during cell spreading on the slide. The problem of the subtelomeric T-banding density patterns found in the endoreduplicated chromosomes and their relation to the structure and molecular composition of this region is briefly discussed.

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