scholarly journals Transplantation of human-induced pluripotent stem cells carried by self-assembling peptide nanofiber hydrogel improves bone regeneration in rat calvarial bone defects

BDJ Open ◽  
2016 ◽  
Vol 2 (1) ◽  
Author(s):  
Kamichika Hayashi ◽  
Hiromi Ochiai-Shino ◽  
Takeaki Shiga ◽  
Shoko Onodera ◽  
Akiko Saito ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Regeneration ◽  
Induced Pluripotent Stem Cells ◽  
Pluripotent Stem Cells ◽  
Bone Defects ◽  
Calvarial Bone ◽  
Self Assembling ◽  
Induced Pluripotent

The Application of Induced Pluripotent Stem Cells for Bone Regeneration: Current Progress and Prospects

2014 ◽  
Vol 20 (4) ◽  
pp. 328-339 ◽  
Author(s):  
Songsong Teng ◽  
Chaoxu Liu ◽  
Christian Krettek ◽  
Michael Jagodzinski
Keyword(s):  
Stem Cells ◽  
Bone Regeneration ◽  
Induced Pluripotent Stem Cells ◽  
Pluripotent Stem Cells ◽  
Induced Pluripotent

Concise review; the recent methods that enhance the osteogenic differentiation of human induced pluripotent stem cells

2021 ◽  
Vol 16 ◽  
Author(s):  
Yongchun Hou ◽  
Zi Yan ◽  
Zhongqi Wu
Keyword(s):  
Stem Cells ◽  
Regenerative Medicine ◽  
Induced Pluripotent Stem Cells ◽  
Osteogenic Differentiation ◽  
Pluripotent Stem Cells ◽  
Bone Defects ◽  
Rapid Expansion ◽  
Differentiation Potential ◽  
Skeletal Defects ◽  
Induced Pluripotent

Bone regeneration is a critical problem in modern clinical practice. Osteocytes are the most abundant cell population of mature adult bone that plays major roles in the regulation of bone formation. In humans, the segmental bone defects cannot be repaired by endogenous regenerative mechanisms. Bone tissue engineering (BTE) is a promising option for the treatment of difficult segmental and skeletal defects. BTE requires suitable cell sources with rapid expansion and adequate function, inducible factors, and scaffolds, to successfully regenerate or repair the bone injury. To overcome the disadvantages of using allogeneic and autologous tissue grafts, stem cell-based therapy has progressed an advanced topic in regenerative medicine. In the past few decades, numerous attempts have been made to generate osteocytes by using pluripotent stem cells (PSCs) for repair and regeneration of bone defects. Human induced pluripotent stem cells (hiPSCs) are PSCs that can self-renew and differentiate into a variety of cell types. Reprogramming of human somatic cells into hiPSCs provides a new opportunity for regenerative medicine, cell-based drug discovery, disease modeling, and toxicity assessment. The ability to differentiate hiPSCs towards mesenchymal stem cells (iPSC-MSCs) is essential for treating bone-related damages and injuries. Several in vitro studies revealed that the cell type of origin for iPSCs, a combination of transcription factors, the type of promoter in the vector, transduction methods, scaffolds, differentiating techniques, and culture medium may affect the osteogenic differentiation potential of hiPSCs. This review will focus on several factors that influence the osteogenic differentiation of human iPSCs.

Cardiac differentiation potential of human induced pluripotent stem cells in a 3D self-assembling peptide scaffold

2015 ◽  
Vol 90 (4-5) ◽  
pp. 101-110 ◽  
Author(s):  
Veronica A.C. Puig-Sanvicens ◽  
Carlos E. Semino ◽  
Nicole I. zur Nieden
Keyword(s):  
Stem Cells ◽  
Induced Pluripotent Stem Cells ◽  
Pluripotent Stem Cells ◽  
Cardiac Differentiation ◽  
Differentiation Potential ◽  
Self Assembling ◽  
Induced Pluripotent

scholarly journals Human Induced Pluripotent Stem Cells Differentiate Into Functional Mesenchymal Stem Cells and Repair Bone Defects

2016 ◽  
Vol 5 (11) ◽  
pp. 1447-1460 ◽  
Author(s):  
Dmitriy Sheyn ◽  
Shiran Ben-David ◽  
Galina Shapiro ◽  
Sandra De Mel ◽  
Maxim Bez ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Induced Pluripotent Stem Cells ◽  
Pluripotent Stem Cells ◽  
Bone Defects ◽  
Induced Pluripotent

Efficient derivation of osteoprogenitor cells from induced pluripotent stem cells for bone regeneration

2014 ◽  
Vol 38 (9) ◽  
pp. 1779-1785 ◽  
Author(s):  
Yoshihiro Dogaki ◽  
Sang Yang Lee ◽  
Takahiro Niikura ◽  
Takashi Iwakura ◽  
Etsuko Okumachi ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Regeneration ◽  
Induced Pluripotent Stem Cells ◽  
Pluripotent Stem Cells ◽  
Osteoprogenitor Cells ◽  
Induced Pluripotent

scholarly journals Size-Optimized Microspace Culture Facilitates Differentiation of Mouse Induced Pluripotent Stem Cells into Osteoid-Rich Bone Constructs

2020 ◽  
Vol 2020 ◽  
pp. 1-14 ◽  
Author(s):  
Phoonsuk Limraksasin ◽  
Hiroko Okawa ◽  
Maolin Zhang ◽  
Takeru Kondo ◽  
Thanaphum Osathanon ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Regeneration ◽  
Induced Pluripotent Stem Cells ◽  
Osteogenic Differentiation ◽  
Pluripotent Stem Cells ◽  
Optimal Size ◽  
Type I ◽  
Gingival Fibroblast ◽  
Osteogenic Induction ◽  
Induced Pluripotent

Microspace culture is promising for self-organization of induced pluripotent stem cells (iPSCs). However, the optimal size of microspaces for osteogenic differentiation is unclear. We hypothesized that a specific microspace size could facilitate self-organizing iPSC differentiation to form bone-like tissue in vitro. The objectives of this study were to investigate such effects of microspace size and to evaluate bone regeneration upon transplantation of the resulting osteogenic constructs. Dissociated mouse gingival fibroblast-derived iPSCs were plated in ultra-low-attachment microspace culture wells containing hundreds of U-bottom-shaped microwell spots per well to form cell aggregates in growth medium. The microwells had different aperture diameters/depths (400/560 μm (Elp400), 500/700 μm (Elp500), and 900/700 μm (Elp900)) (Kuraray; Elplasia). After 5 days of aggregation, cells were maintained in osteogenic induction medium for 35 days. Only cells in the Elp500 condition tightly aggregated and maintained high viability during osteogenic induction. After 10 days of induction, Elp500 cell constructs showed significantly higher gene expression of Runx2, Osterix, Collagen 1a1, Osteocalcin, Bone sialoprotein, and Osteopontin compared to constructs in Elp400 and Elp900. In methylene blue-counterstained von Kossa staining and Movat’s pentachrome staining, only Elp500 constructs showed robust osteoid formation on day 35, with high expression of type I collagen (a major osteoid component) and osteocalcin proteins. Cell constructs were transplanted into rat calvarial bone defects, and micro-CT analysis after 3 weeks showed better bone repair with significantly higher bone mineral density in the Elp500 group compared to the Elp900 group. These results suggest that microspace size affects self-organized osteogenic differentiation of iPSCs. Elp500 microspace culture specifically induces mouse iPSCs into osteoid-rich bone-like tissue possessing high bone regeneration capacity.

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